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AB305304

Anti-Aquaporin 5 antibody [EPR27474-70] - BSA and Azide free

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(2 Publications)

Rabbit Recombinant Monoclonal Aquaporin 5 antibody. Carrier free. Suitable for WB, IHC-P, IHC-Fr, IP and reacts with Mouse, Rat samples. Cited in 2 publications.

View Alternative Names

Aquaporin-5, AQP-5, Aqp5

11 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aquaporin 5 antibody [EPR27474-70] - BSA and Azide free (AB305304)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aquaporin 5 antibody [EPR27474-70] - BSA and Azide free (AB305304)
Immunohistochemistry (Frozen sections) - Anti-Aquaporin 5 antibody [EPR27474-70] - BSA and Azide free (AB305304)
  • IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-Aquaporin 5 antibody [EPR27474-70] - BSA and Azide free (AB305304)
Immunohistochemistry (Frozen sections) - Anti-Aquaporin 5 antibody [EPR27474-70] - BSA and Azide free (AB305304)
  • IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-Aquaporin 5 antibody [EPR27474-70] - BSA and Azide free (AB305304)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aquaporin 5 antibody [EPR27474-70] - BSA and Azide free (AB305304)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aquaporin 5 antibody [EPR27474-70] - BSA and Azide free (AB305304)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aquaporin 5 antibody [EPR27474-70] - BSA and Azide free (AB305304)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aquaporin 5 antibody [EPR27474-70] - BSA and Azide free (AB305304)
Immunohistochemistry (Frozen sections) - Anti-Aquaporin 5 antibody [EPR27474-70] - BSA and Azide free (AB305304)
  • IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-Aquaporin 5 antibody [EPR27474-70] - BSA and Azide free (AB305304)

This data was developed using ab303506, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse lung (fresh) tissue labeling Aquaporin 5 with ab305303 at 1/100 (5.3 µg/ml) dilution followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (Green). Confocal image showing positive staining on mouse lung. The nuclear counterstain was DAPI (Blue). The section was incubated with ab305303 for 60 mins at room temperature. The section was then mounted using Fluoromount®.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). The nuclear counterstain was DAPI (Blue). Secondary antibody control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution.

Immunohistochemistry (Frozen sections) - Anti-Aquaporin 5 antibody [EPR27474-70] - BSA and Azide free (AB305304)
  • IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-Aquaporin 5 antibody [EPR27474-70] - BSA and Azide free (AB305304)
Immunoprecipitation - Anti-Aquaporin 5 antibody [EPR27474-70] - BSA and Azide free (AB305304)
  • IP

Supplier Data

Immunoprecipitation - Anti-Aquaporin 5 antibody [EPR27474-70] - BSA and Azide free (AB305304)

This data was developed using ab305303, the same antibody clone in a different buffer formulation. Aquaporin 5 was immunoprecipitated from 0.35 mg Rat lung tissue lysate 10 μg with ab305303 at 1/30 dilution (2μg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab305303. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution. Lane 1 : Rat lung tissue lysate 10 μg Lane 2 : ab305303 IP in Rat lung tissue lysate Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab305303 in Rat lung tissue lysate Blocking and dilution buffer and concentration : 5% NFDM/TBST. Exposure time : 15 seconds Samples are non-boiled as boiling may cause protein aggregation.

All lanes:

Immunoprecipitation - Anti-Aquaporin 5 antibody [EPR27474-70] (<a href='/en-us/products/primary-antibodies/aquaporin-5-antibody-epr27474-70-ab305303'>ab305303</a>) at 1/30 dilution

All lanes:

Rat lung tissue

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (<a href='/en-us/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/5000 dilution

Observed band size: 24 kDa

false

Exposure time: 15s

Immunoprecipitation - Anti-Aquaporin 5 antibody [EPR27474-70] - BSA and Azide free (AB305304)
  • IP

Supplier Data

Immunoprecipitation - Anti-Aquaporin 5 antibody [EPR27474-70] - BSA and Azide free (AB305304)

This data was developed using ab305303, the same antibody clone in a different buffer formulation. Aquaporin 5 was immunoprecipitated from 0.35 mg Mouse lung tissue lysate 10 μg with ab305303 at 1/30 dilution (2μg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab305303. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution. Lane 1 : Mouse lung tissue lysate 10 μg Lane 2 : ab305303 IP in Mouse lung tissue lysate Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab305303 in Mouse lung tissue lysate Blocking and dilution buffer and concentration : 5% NFDM/TBST. Exposure time : 3 seconds Samples are non-boiled as boiling may cause protein aggregation.

All lanes:

Immunoprecipitation - Anti-Aquaporin 5 antibody [EPR27474-70] (<a href='/en-us/products/primary-antibodies/aquaporin-5-antibody-epr27474-70-ab305303'>ab305303</a>) at 1/30 dilution

All lanes:

Mouse lung tissue

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (<a href='/en-us/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/5000 dilution

Observed band size: 24 kDa

false

Exposure time: 3s

Western blot - Anti-Aquaporin 5 antibody [EPR27474-70] - BSA and Azide free (AB305304)
  • WB

Supplier Data

Western blot - Anti-Aquaporin 5 antibody [EPR27474-70] - BSA and Azide free (AB305304)

This data was developed using ab305303, the same antibody clone in a different buffer formulation. Blocking and diluting buffer and concentration : 5% NFDM/TBST Negative control : kidney (PMID : 9767520). Exposure time : 48 seconds

All lanes:

Western blot - Anti-Aquaporin 5 antibody [EPR27474-70] (<a href='/en-us/products/primary-antibodies/aquaporin-5-antibody-epr27474-70-ab305303'>ab305303</a>) at 1/1000 dilution

Lane 1:

Mouse lu tissue lysate prepared in RIPA lysis method at 20 µg

Lane 2:

Rat lu tissue lysate prepared in RIPA lysis method at 20 µg

Lane 3:

Mouse kidney tissue lysate prepared in RIPA lysis method at 20 µg

Lane 4:

Rat kidney tissue lysate prepared in RIPA lysis method at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/50000 dilution

Observed band size: 24 kDa

false

Exposure time: 48s

Western blot - Anti-Aquaporin 5 antibody [EPR27474-70] - BSA and Azide free (AB305304)
  • WB

Supplier Data

Western blot - Anti-Aquaporin 5 antibody [EPR27474-70] - BSA and Azide free (AB305304)

This data was developed using ab305303, the same antibody clone in a different buffer formulation. Blocking and diluting buffer and concentration : 5% NFDM/TBST Negative control : cerebellum (PMID : 9767520), heart (PMID : 9767520). Exposure time : 70 seconds

All lanes:

Western blot - Anti-Aquaporin 5 antibody [EPR27474-70] (<a href='/en-us/products/primary-antibodies/aquaporin-5-antibody-epr27474-70-ab305303'>ab305303</a>) at 1/1000 dilution

Lane 1:

Mouse salivary gland tissue lysate prepared in RIPA lysis method at 20 µg

Lane 2:

Mouse cerebellum tissue lysate prepared in RIPA lysis method at 20 µg

Lane 3:

Mouse heart tissue lysate prepared in RIPA lysis method at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/50000 dilution

Observed band size: 24 kDa

false

Exposure time: 70s

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR27474-70

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Rat

Applications

WB, IP, IHC-Fr, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.", "IHCFr-species-checked": "testedAndGuaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "<p></p>", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.", "IHCFr-species-checked": "testedAndGuaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "<p></p>", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>" } } }
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Product details

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The protein known as Aquaporin 5 often abbreviated as AQP5 is a member of the aquaporin family which are integral membrane proteins that function as channels for water. This particular protein facilitates water transport across cell membranes an action critical in maintaining fluid balance. Aquaporin 5 has a molecular mass of approximately 29 kDa. It is largely expressed in epithelial cells in various tissues including the salivary glands lacrimal glands and respiratory tissues. It is recognized by different names such as Aquaporin-5 and sometimes referenced in studies as Aquazol 5.
Biological function summary

Water transport and secretion processes heavily rely on the presence of Aquaporin 5. It is integral in the formation of tight junctions in epithelial cells enhancing cell permeability to water. Unlike some other aquaporins Aquaporin 5 operates independently and is not known to be part of a larger protein complex. Its presence influences the efficiency of fluid secretion affecting activities such as salivation and tear secretion. The capacity of AQP5 to control water movement tightly connects it to cellular hydration homeostasis.

Pathways

Aquaporin 5 is significantly involved in the regulation of water balance and osmotic equilibrium in epithelial tissues. Its role in fluid secretion pathways is interconnected with other proteins like Aquaporin 4 which share similar duties in the osmotic control of water. In the water transport pathways AQP5 coordinates with proteins like CFTR (Cystic Fibrosis Transmembrane Conductance Regulator) to manage fluid movement particularly in the respiratory passages and glands. The precise regulation of water flow by these pathways ensures that overall tissue hydration remains constant.

Aquaporin 5 is directly associated with conditions such as Sjögren's syndrome and xerostomia (dry mouth syndrome). During Sjögren's syndrome malfunction of AQP5 can lead to impaired salivary and tear secretion contributing to the symptoms of dryness. Furthermore defects or polymorphisms in AQP5 have implications in respiratory diseases potentially affecting proteins like Aquaporin 3 which shares functional similarities. Understanding the role of AQP5 in these conditions enables further exploration into therapeutic targets and treatment methods.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Aquaporins form homotetrameric transmembrane channels, with each monomer independently mediating water transport across the plasma membrane along its osmotic gradient (By similarity). Plays an important role in fluid secretion in salivary glands (PubMed : 10400615, PubMed : 16571723, PubMed : 18027168). Required for TRPV4 activation by hypotonicity. Together with TRPV4, controls regulatory volume decrease in salivary epithelial cells (PubMed : 16571723). Seems to play a redundant role in water transport in the eye, lung and in sweat glands (PubMed : 10619865, PubMed : 12042359, PubMed : 18027168).
See full target information Aqp5
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Cellular & molecular biology letters 29:147 PubMed39623319

2024

Salivary gland protective and antiinflammatory effects of genistein in Sjögren's syndrome by inhibiting Xist/ACSL4-mediated ferroptosis following binding to estrogen receptor-alpha.

Applications

Unspecified application

Species

Unspecified reactive species

Tianjiao Mao,Wei Wei,Bo Chen,Yixin Chen,Shuqi Liang,Guiping Chen,Zhuoyuan Liu,Xiaodan Wu,Lihong Wu,Xiaomeng Li,Nobumoto Watanabe,Kevin H Mayo,Janak L Pathak,Jiang Li

Heliyon 10:e33860 PubMed39027572

2024

AutoDock and molecular dynamics-based therapeutic potential prediction of flavonoids for primary Sjögren's syndrome.

Applications

Unspecified application

Species

Unspecified reactive species

Tianjiao Mao,Bo Chen,Wei Wei,Guiping Chen,Zhuoyuan Liu,Lihong Wu,Xiaomeng Li,Janak L Pathak,Jiang Li
View all publications
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