AB92320

Anti-Aquaporin 5 antibody [EPR3747]

Name: Anti-Aquaporin 5 antibody [EPR3747] (ab92320) | Abcam
Brand: Abcam Limited
SKU: AB92320
Price: 405 USD
Availability: InStock
Rating: 4 (7 reviews)

RabMAb
Recombinant
What is this?

(7 Reviews)

(67 Publications)

Anti-Aquaporin 5 antibody [EPR3747] (ab92320) is a rabbit monoclonal antibody detecting Aquaporin 5 in Western Blot, IHC-P. Suitable for Human.

- Biophysical QC for unrivalled batch-batch consistency
- Over 50 publications
- Trusted since 2010

View Alternative Names

Aquaporin-5, AQP-5, AQP5

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aquaporin 5 antibody [EPR3747] (AB92320)

IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aquaporin 5 antibody [EPR3747] (AB92320)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human stomach tissue sections labelling Aquaporin 5 with purified ab92320 at 1/1200 dilution (0.13 μg/ml). Heat mediated antigen retrieval was performed using Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) secondary antibody was used at 1/0 dilution. PBS instead of the primary antibody was used as the negative control.

Lab

Western blot - Anti-Aquaporin 5 antibody [EPR3747] (AB92320)

1%SDS Hot lysis method is recommended for this antibody. The molecular weight observed is consistent with what has been described in the literatures (PMID : 32901888, 23313152).

All lanes:

Western blot - Anti-Aquaporin 5 antibody [EPR3747] (ab92320) at 1/1000 dilution

Lane 1:

SW480 (Human colorectal adenocarcinoma epithelial cell) prepared in RIPA lysis method whole cell lysate at 20 µg

Lane 2:

SW480 (Human colorectal adenocarcinoma epithelial cell) prepared in 1%SDS Hot lysis method whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 28 kDa

Observed band size: 24 kDa

false

Carrier free

Anti-Aquaporin 5 antibody [EPR3747] - BSA and Azide free
519 Alexa Fluor® 488

Alexa Fluor® 488 Anti-Aquaporin 5 antibody [EPR3747]
565 Alexa Fluor® 555

Alexa Fluor® 555 Anti-Aquaporin 5 antibody [EPR3747]
665 Alexa Fluor® 647

Alexa Fluor® 647 Anti-Aquaporin 5 antibody [EPR3747]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR3747

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/1200", "IHCP-species-notes": "Antigen retrieval is recommended before commencing with IHC staining protocol. The use of an HRP/AP polymerized secondary antibody is recommended as stronger signals have been found using these.For unpurified use between 1/100 and 1/250. Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "" } } }

style

left-column

Product details

What is this antibody validated in?
Anti-Aquaporin 5 antibody [EPR3747] (ab92320) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Immunohistochemistry (IHC-P) in Human samples.

What is the molecular weight of Aquaporin 5?
Anti-Aquaporin 5 [EPR3747] (ab92320) specifically detects a band for Aquaporin 5 (UniProt: P55064) at a molecular weight of 28kDa.

Trusted by the scientific community
Anti-Aquaporin 5 [EPR3747] (ab92320) was first used in a scientific publication in 2010 and has been cited over 50 times in peer-reviewed journals.

Reviewed by scientists
Anti-Aquaporin 5 [EPR3747] (ab92320) has over 5 independent reviews from customers.

Trial sizes available!
Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies.

Other related products
We have a range of other formats of antibody clone [EPR3747] also available for your convenience: ab92320, Alexa Fluor® 647 - ab215225, Carrier free - ab239904, Alexa Fluor® 555 - ab307863

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Stable for 12 months at -20°C

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The protein known as Aquaporin 5 often abbreviated as AQP5 is a member of the aquaporin family which are integral membrane proteins that function as channels for water. This particular protein facilitates water transport across cell membranes an action critical in maintaining fluid balance. Aquaporin 5 has a molecular mass of approximately 29 kDa. It is largely expressed in epithelial cells in various tissues including the salivary glands lacrimal glands and respiratory tissues. It is recognized by different names such as Aquaporin-5 and sometimes referenced in studies as Aquazol 5.

Biological function summary

Water transport and secretion processes heavily rely on the presence of Aquaporin 5. It is integral in the formation of tight junctions in epithelial cells enhancing cell permeability to water. Unlike some other aquaporins Aquaporin 5 operates independently and is not known to be part of a larger protein complex. Its presence influences the efficiency of fluid secretion affecting activities such as salivation and tear secretion. The capacity of AQP5 to control water movement tightly connects it to cellular hydration homeostasis.

Pathways

Aquaporin 5 is significantly involved in the regulation of water balance and osmotic equilibrium in epithelial tissues. Its role in fluid secretion pathways is interconnected with other proteins like Aquaporin 4 which share similar duties in the osmotic control of water. In the water transport pathways AQP5 coordinates with proteins like CFTR (Cystic Fibrosis Transmembrane Conductance Regulator) to manage fluid movement particularly in the respiratory passages and glands. The precise regulation of water flow by these pathways ensures that overall tissue hydration remains constant.

Aquaporin 5 is directly associated with conditions such as Sjögren's syndrome and xerostomia (dry mouth syndrome). During Sjögren's syndrome malfunction of AQP5 can lead to impaired salivary and tear secretion contributing to the symptoms of dryness. Furthermore defects or polymorphisms in AQP5 have implications in respiratory diseases potentially affecting proteins like Aquaporin 3 which shares functional similarities. Understanding the role of AQP5 in these conditions enables further exploration into therapeutic targets and treatment methods.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Aquaporins form homotetrameric transmembrane channels, with each monomer independently mediating water transport across the plasma membrane along its osmotic gradient (PubMed : 18768791, PubMed : 8621489). Plays an important role in fluid secretion in salivary glands (By similarity). Required for TRPV4 activation by hypotonicity. Together with TRPV4, controls regulatory volume decrease in salivary epithelial cells (PubMed : 16571723). Seems to play a redundant role in water transport in the eye, lung and in sweat glands (By similarity).

See full target information AQP5

style

left-column

Publications (67)

Recent publications for all applications. Explore the full list and refine your search

Communications biology 8:1025 PubMed40634525

2025

Triggering AHR resolves TGF-β1 induced fibroblast activation and promotes AT1 cell regeneration in alveolar organoids.

Applications

Unspecified application

Species

Unspecified reactive species

Andrew S Hagan,Scott Williams,Casey J N Mathison,Shanshan Yan,Bao Nguyen,Glenn C Federe,Guray Kuzu,Joseph C Siefert,Janice Hampton,Victor Chichkov,S Whitney Barnes,Frederick J King,Brandon L Taylor,John R Walker,Rui Zhao,Jimmy Elliott,Dean P Phillips,Bin Fang,Rebekah S Decker

Cell regeneration (London, England) 14:18 PubMed40377813

2025

The critical role of BMP signaling in gastric epithelial cell differentiation revealed by organoids.

Applications

Unspecified application

Species

Unspecified reactive species

Fan Hong,Xiaodan Wang,Nanshan Zhong,Ze Zhang,Shibo Lin,Mengxian Zhang,Haonan Li,Yuan Liu,Yalong Wang,Lianzheng Zhao,Xiao Yang,Hongwen Zhou,Hui Liang,Ye-Guang Chen

Nature communications 16:4369 PubMed40350470

2025

Basal-shift transformation leads to EGFR therapy-resistance in human lung adenocarcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Taro Shinozaki,Kazuhiro Togasaki,Junko Hamamoto,Akifumi Mitsuishi,Takahiro Fukushima,Kai Sugihara,Toshiki Ebisudani,Masahiko Okada,Ayaka Saito,Lisa Shigematsu,Hatsuyo Takaoka,Fumimaro Ito,Keiko Ohgino,Kota Ishioka,Kageaki Watanabe,Tsunekazu Hishima,Yutaka Kurebayashi,Katsura Emoto,Hideki Terai,Shinnosuke Ikemura,Ichiro Kawada,Keisuke Asakura,Tomoyuki Hishida,Hisao Asamura,Yuki Ohta,Sirirat Takahashi,Mayumi Oda,Megumu Saito,Mami Matano,Kenzo Soejima,Masayuki Fujii,Koichi Fukunaga,Hiroyuki Yasuda,Toshiro Sato

Nature communications 16:1303 PubMed39900892

2025

Cloned airway basal progenitor cells to repair fibrotic lung through re-epithelialization.

Applications

Unspecified application

Species

Unspecified reactive species

Yu Zhao,Yueqing Zhou,Weipan Zhang,Mingzhe Liu,Jun Duan,Xiaopeng Zhang,Qiwang Ma,Yujia Wang,Yuzhen Zhang,Zhongliang Guo,Ting Zhang,Wei Zuo

Cell reports. Medicine 5:101819 PubMed39566467

2024

Autologous transplantation of P63 lung progenitor cells in patients with bronchiectasis: A randomized, single-blind, controlled trial.

Applications

Unspecified application

Species

Unspecified reactive species

Jiayang Yan,Weipan Zhang,Yun Feng,Xuefei Liu,Lingyun Niu,Yi Guo,Ling Zhou,Mengmeng Shi,Caixia Di,Qiurui Zhang,Xiaofei Wang,Jianping Zhou,Ranran Dai,Lei Ni,Zhiyao Bao,Tianli Yan,Yun Hu,Ping Wang,Ting Zhang,Min Zhou,Wei Zuo,Jieming Qu

Function (Oxford, England) 6: PubMed39479800

2024

Loss of STIM1 and STIM2 in Salivary Glands Disrupts ANO1 Function but Does Not Induce Sjogren's Disease.

Applications

Unspecified application

Species

Unspecified reactive species

Ga-Yeon Son,Anna Zou,Amanda Wahl,Kai Ting Huang,Saruul Zorgit,Manikandan Vinu,Fang Zhou,Larry Wagner,Youssef Idaghdour,David I Yule,Stefan Feske,Rodrigo S Lacruz

Scientific reports 14:21784 PubMed39294273

2024

Unveiling senescence-associated ocular pathogenesis via lacrimal gland organoid magnetic bioassembly platform and HMGB1-Box A gene therapy.

Applications

Unspecified application

Species

Unspecified reactive species

Joao Nuno Ferreira,Narumol Bhummaphan,Risa Chaisuparat,Toan Van Phan,Yamin Oo,Pimkwan Jaru-Ampornpan,Oranart Matangkasombut,Apiwat Mutirangura

BMC molecular and cell biology 25:9 PubMed38500038

2024

Development of an in vitro human alveolar epithelial air-liquid interface model using a small molecule inhibitor cocktail.

Applications

Unspecified application

Species

Unspecified reactive species

Ikuya Tanabe,Kanae Ishimori,Shinkichi Ishikawa

International journal of cancer 154:1814-1827 PubMed38282121

2024

TGFβ-induced EN1 promotes tumor budding of adenoid cystic carcinoma in patient-derived organoid model.

Applications

Unspecified application

Species

Unspecified reactive species

Zhixuan Hui,Bo Wang,Zhengyan Liu,Jinhui Wei,Jiaxing Gan,Marene Landstrom,Yabing Mu,Guangxiang Zang

Cell regeneration (London, England) 12:27 PubMed37525021

2023

Cross-species single-cell transcriptomic analysis of animal gastric antrum reveals intense porcine mucosal immunity.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaodan Wang,Fan Hong,Haonan Li,Yalong Wang,Mengxian Zhang,Shibo Lin,Hui Liang,Hongwen Zhou,Yuan Liu,Ye-Guang Chen

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Aquaporin 5 antibody [EPR3747]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aquaporin 5 antibody [EPR3747] (AB92320)

Western blot - Anti-Aquaporin 5 antibody [EPR3747] (AB92320)

Related conjugates and formulations (4)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (67)

Alternative Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Complementary Products

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Product promise