Rabbit Polyclonal ARAP1 antibody. Suitable for IP, WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human ARAP1 aa 1150-1250.
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Publications
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- Cell reports 34:1087032021Chromatin 3D interaction analysis of the STARD10 locus unveils FCHSD2 as a regulator of insulin secretion.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMing Hu,Inês Cebola,Gaelle Carrat,Shuying Jiang,Sameena Nawaz,Amna Khamis,Mickaël Canouil,Philippe Froguel,Anke Schulte,Michele Solimena,Mark Ibberson,Piero Marchetti,Fabian L Cardenas-Diaz,Paul J Gadue,Benoit Hastoy,Leonardo Almeida-Souza,Harvey McMahon,Guy A RutterPubMed 33535042
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7 - 8
Preservative: 0.09% Sodium azide
Constituents: Tris citrate/phosphate- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human ARAP1 aa 1150-1250. The exact immunogen used to generate this antibody is proprietary information. Database link Q96P48
Reactivity data
Select an application| IP | WB | |
|---|---|---|
| Human | Tested | Tested |
| Bat | Predicted | Predicted |
| Cow | Predicted | Predicted |
| Dog | Predicted | Predicted |
| Elephant | Predicted | Predicted |
| Gorilla | Predicted | Predicted |
| Orangutan | Predicted | Predicted |
| Pig | Predicted | Predicted |
| Rabbit | Predicted | Predicted |
| Rhesus monkey | Predicted | Predicted |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 2.00000-5.00000 µg/mg of lysate | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rabbit, Cow, Dog, Pig, Rhesus monkey, Gorilla, Orangutan, Bat, Elephant | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/2000.00000 - 1/10000.00000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rabbit, Cow, Dog, Pig, Rhesus monkey, Gorilla, Orangutan, Bat, Elephant | Dilution info - | Notes - |
Target data
Function
Phosphatidylinositol 3,4,5-trisphosphate-dependent GTPase-activating protein that modulates actin cytoskeleton remodeling by regulating ARF and RHO family members. Is activated by phosphatidylinositol 3,4,5-trisphosphate (PtdIns(3,4,5)P3) binding. Can be activated by phosphatidylinositol 3,4-bisphosphate (PtdIns(3,4,5)P2) binding, albeit with lower efficiency. Has a preference for ARF1 and ARF5 (By similarity).
Alternative names
CENTD2, KIAA0782, ARAP1, Centaurin-delta-2, Cnt-d2
Recommended products
Rabbit Polyclonal ARAP1 antibody. Suitable for IP, WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human ARAP1 aa 1150-1250.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7 - 8
Preservative: 0.09% Sodium azide
Constituents: Tris citrate/phosphate- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human ARAP1 aa 1150-1250. The exact immunogen used to generate this antibody is proprietary information. Database link Q96P48
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The ARAP1 protein also known as Centaurin delta-1 plays a role in mechanics acting as an ArfGAP with RhoGAP domain and Ankyrin repeat protein. ARAP1 weighs approximately 150 kDa and mainly expresses in tissues including the heart liver and skeletal muscle. This protein localizes primarily in the cytoplasm and is involved in cellular processes by regulating phosphoinositide 3-kinase (PI3K) signaling.
- Biological function summary
ARAP1 coordinates the remodeling of the actin cytoskeleton. It assists in cell adhesion migration and endocytosis. Through its GAP domains ARAP1 regulates small GTPases such as Arf and Rho proteins which participate in vesicular trafficking and cytoskeletal dynamics. While not a member of a specific complex it interacts with signaling molecules to influence cellular shape and movement.
- Pathways
ARAP1 acts in the PI3K/AKT and Rho signaling pathways shaping cellular responses to external stimuli. It regulates the localization and activity of phosphoinositide phosphates important for the PI3K/AKT pathway. ARAP1's interaction with proteins like Akt and RhoA affects their signaling to produce changes in cell motility and growth integrating signals from various upstream activators.
- Associated diseases and disorders
ARAP1 has links to cancer and type 2 diabetes. In cancer ARAP1 may contribute to tumor cell invasiveness relating to its function in actin cytoskeleton regulation and its interaction with RhoA. Additionally its association with PI3K/AKT pathway dysregulation connects ARAP1 to insulin signaling disruption relevant in type 2 diabetes. Understanding these interactions may provide insights into therapeutic strategies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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2 product images
Western blot - Anti-ARAP1 antibody (ab99382)
All lanes: Western blot - Anti-ARAP1 antibody (ab99382) at 0.1 µg/mL
Lane 1: HeLa whole cell lysate at 50 µg
Lane 2: HeLa whole cell lysate at 15 µg
Lane 3: HeLa whole cell lysate at 5 µg
Predicted band size: 162 kDa
Exposure time: 30s
Immunoprecipitation - Anti-ARAP1 antibody (ab99382)
Detection of ARAP1 by Western Blot of Immunprecipitate.
ab99382 at 1µg/ml staining ARAP1 in HeLa whole cell lysate immunoprecipitated using ab99382 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Detection: Chemiluminescence with exposure time of 10 seconds.All lanes: Immunoprecipitation - Anti-ARAP1 antibody (ab99382)
Predicted band size: 162 kDa
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