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AB99382

Anti-ARAP1 antibody

5

(1 Review)

|

(3 Publications)

Rabbit Polyclonal ARAP1 antibody. Suitable for IP, WB and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human ARAP1 aa 1150-1250.

View Alternative Names

CENTD2, KIAA0782, ARAP1, Centaurin-delta-2, Cnt-d2

2 Images
Immunoprecipitation - Anti-ARAP1 antibody (AB99382)
  • IP

Unknown

Immunoprecipitation - Anti-ARAP1 antibody (AB99382)

Detection of ARAP1 by Western Blot of Immunprecipitate.
ab99382 at 1µg/ml staining ARAP1 in HeLa whole cell lysate immunoprecipitated using ab99382 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Detection : Chemiluminescence with exposure time of 10 seconds.

All lanes:

Immunoprecipitation - Anti-ARAP1 antibody (ab99382)

Predicted band size: 162 kDa

false

Western blot - Anti-ARAP1 antibody (AB99382)
  • WB

Unknown

Western blot - Anti-ARAP1 antibody (AB99382)

All lanes:

Western blot - Anti-ARAP1 antibody (ab99382) at 0.1 µg/mL

Lane 1:

HeLa whole cell lysate at 50 µg

Lane 2:

HeLa whole cell lysate at 15 µg

Lane 3:

HeLa whole cell lysate at 5 µg

Predicted band size: 162 kDa

false

Exposure time: 30s

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IP, WB

applications

Immunogen

Synthetic Peptide within Human ARAP1 aa 1150-1250. The exact immunogen used to generate this antibody is proprietary information.

Q96P48

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-5 µg/mg of lysate", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "<p></p>" }, "Bat": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Dog": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Elephant": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Gorilla": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Pig": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rabbit": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rhesus monkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: Tris citrate/phosphate
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The ARAP1 protein also known as Centaurin delta-1 plays a role in mechanics acting as an ArfGAP with RhoGAP domain and Ankyrin repeat protein. ARAP1 weighs approximately 150 kDa and mainly expresses in tissues including the heart liver and skeletal muscle. This protein localizes primarily in the cytoplasm and is involved in cellular processes by regulating phosphoinositide 3-kinase (PI3K) signaling.
Biological function summary

ARAP1 coordinates the remodeling of the actin cytoskeleton. It assists in cell adhesion migration and endocytosis. Through its GAP domains ARAP1 regulates small GTPases such as Arf and Rho proteins which participate in vesicular trafficking and cytoskeletal dynamics. While not a member of a specific complex it interacts with signaling molecules to influence cellular shape and movement.

Pathways

ARAP1 acts in the PI3K/AKT and Rho signaling pathways shaping cellular responses to external stimuli. It regulates the localization and activity of phosphoinositide phosphates important for the PI3K/AKT pathway. ARAP1's interaction with proteins like Akt and RhoA affects their signaling to produce changes in cell motility and growth integrating signals from various upstream activators.

ARAP1 has links to cancer and type 2 diabetes. In cancer ARAP1 may contribute to tumor cell invasiveness relating to its function in actin cytoskeleton regulation and its interaction with RhoA. Additionally its association with PI3K/AKT pathway dysregulation connects ARAP1 to insulin signaling disruption relevant in type 2 diabetes. Understanding these interactions may provide insights into therapeutic strategies.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Phosphatidylinositol 3,4,5-trisphosphate-dependent GTPase-activating protein that modulates actin cytoskeleton remodeling by regulating ARF and RHO family members. Is activated by phosphatidylinositol 3,4,5-trisphosphate (PtdIns(3,4,5)P3) binding. Can be activated by phosphatidylinositol 3,4-bisphosphate (PtdIns(3,4,5)P2) binding, albeit with lower efficiency. Has a preference for ARF1 and ARF5 (By similarity).
See full target information ARAP1
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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Advanced science (Weinheim, Baden-Wurttemberg, Germany) 10:e2303545 PubMed37963851

2023

O-GlcNAcylation Regulates Centrosome Behavior and Cell Polarity to Reduce Pulmonary Fibrosis and Maintain the Epithelial Phenotype.

Applications

Unspecified application

Species

Unspecified reactive species

Fan Yu,Song Yang,Hua Ni,Dai Heng,Xuemei Wu,Mulin Yang,Xinming Zhang,Yuxin Cao,Yandong Pei,Di An,Dengwen Li,Dayong Liu,Lin Liu,Leiting Pan,Quan Chen,Xueliang Zhu,Jun Zhou

Bioengineered 13:7868-7880 PubMed35291911

2022

Long noncoding RNA ArfGAP with RhoGAP domain, ankyrin repeat and PH domain 1 antisense RNA 1 recruits enhancer of zeste 2 polycomb repressive complex 2 subunit to promote the proliferation, migration and invasion of lung adenocarcinoma cells.

Applications

Unspecified application

Species

Unspecified reactive species

Jinyuan Liu,Chunfeng Pan,Rongxin Lu,Shijiang Zhang

Cell reports 34:108703 PubMed33535042

2021

Chromatin 3D interaction analysis of the STARD10 locus unveils FCHSD2 as a regulator of insulin secretion.

Applications

Unspecified application

Species

Unspecified reactive species

Ming Hu,Inês Cebola,Gaelle Carrat,Shuying Jiang,Sameena Nawaz,Amna Khamis,Mickaël Canouil,Philippe Froguel,Anke Schulte,Michele Solimena,Mark Ibberson,Piero Marchetti,Fabian L Cardenas-Diaz,Paul J Gadue,Benoit Hastoy,Leonardo Almeida-Souza,Harvey McMahon,Guy A Rutter
View all publications
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