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Rabbit Recombinant Monoclonal ARC antibody. Suitable for IHC-P, IP, WB and reacts with Mouse, Rat, Human samples. Cited in 10 publications.


Images

Western blot - Anti-Arc antibody [EPR18950] (AB183183), expandable thumbnail
  • Western blot - Anti-Arc antibody [EPR18950] (AB183183), expandable thumbnail
  • Western blot - Anti-Arc antibody [EPR18950] (AB183183), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Arc antibody [EPR18950] (AB183183), expandable thumbnail
  • Immunoprecipitation - Anti-Arc antibody [EPR18950] (AB183183), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PIPWB
Human
Expected
Expected
Tested
Mouse
Tested
Tested
Tested
Rat
Expected
Expected
Tested

Tested
Tested

Species
Mouse
Dilution info
1/100
Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Expected
Expected

Species
Rat, Human
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/30
Notes

-

Expected
Expected

Species
Rat, Human
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/1000
Notes

-

Species
Rat
Dilution info
1/1000
Notes

-

Species
Human
Dilution info
1/1000
Notes

-

Associated Products

Select an associated product type

1 product for Alternative Product

Target data

Function

Master regulator of synaptic plasticity that self-assembles into virion-like capsids that encapsulate RNAs and mediate intercellular RNA transfer in the nervous system. ARC protein is released from neurons in extracellular vesicles that mediate the transfer of ARC mRNA into new target cells, where ARC mRNA can undergo activity-dependent translation. ARC capsids are endocytosed and are able to transfer ARC mRNA into the cytoplasm of neurons. Acts as a key regulator of synaptic plasticity: required for protein synthesis-dependent forms of long-term potentiation (LTP) and depression (LTD) and for the formation of long-term memory. Regulates synaptic plasticity by promoting endocytosis of AMPA receptors (AMPARs) in response to synaptic activity: this endocytic pathway maintains levels of surface AMPARs in response to chronic changes in neuronal activity through synaptic scaling, thereby contributing to neuronal homeostasis. Acts as a postsynaptic mediator of activity-dependent synapse elimination in the developing cerebellum by mediating elimination of surplus climbing fiber synapses. Accumulates at weaker synapses, probably to prevent their undesired enhancement. This suggests that ARC-containing virion-like capsids may be required to eliminate synaptic material. Required to transduce experience into long-lasting changes in visual cortex plasticity and for long-term memory (By similarity). Involved in postsynaptic trafficking and processing of amyloid-beta A4 (APP) via interaction with PSEN1 (By similarity). In addition to its role in synapses, also involved in the regulation of the immune system: specifically expressed in skin-migratory dendritic cells and regulates fast dendritic cell migration, thereby regulating T-cell activation (By similarity).

Alternative names

KIAA0278, ARC, Activity-regulated cytoskeleton-associated protein, hArc, Activity-regulated gene 3.1 protein homolog, ARC/ARG3.1, Arg3.1

Recommended products

Rabbit Recombinant Monoclonal ARC antibody. Suitable for IHC-P, IP, WB and reacts with Mouse, Rat, Human samples. Cited in 10 publications.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR18950
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

The Arc protein also known as Activity-regulated cytoskeleton-associated protein weighs approximately 45 kDa. This protein expresses mainly in the brain specifically in the neurons. It is particularly active in the synaptic regions where it plays a role in synaptic plasticity. The Arc protein belongs to an emerging class of proteins that show similarities with viral proteins. Its structural and functional properties help facilitate the dynamic regulation of synapses and neuronal activity.

Biological function summary

The Arc protein influences synaptic plasticity and memory formation. It does not operate alone; instead it interacts within complexes that regulate synaptic strength. These complexes contain other proteins critical for actin cytoskeleton remodeling which impacts synaptic efficacy. Through these interactions Arc supports the internalization of AMPA receptors playing a role in long-term depression (LTD) and long-term potentiation (LTP) of synapses essential mechanisms for learning and memory.

Pathways

The Arc protein forms integral parts of the synaptic signaling pathways. It connects with the BDNF-TrkB signaling pathway which is important for neuronal development and synaptic modulation. Arc also relates to the mTOR signaling pathway which governs cellular growth and protein synthesis. Through these pathways Arc interacts with proteins like Neuro2a and SynGAP highlighting its comprehensive role in synaptic regulation and plasticity.

Associated diseases and disorders

Arc protein has a connection to neurological disorders such as Alzheimer's disease and schizophrenia. Researchers found altered Arc expression and function in the brains of affected individuals. These disorders show a link to disrupted synaptic plasticity where Arc plays a significant role. Arc connects to other proteins like Neuro-2a and Caspase-3 in the pathology of these conditions indicating potential targets for therapeutic intervention.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
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6 product images

  • Western blot - Anti-Arc antibody [EPR18950] (ab183183), expandable thumbnail

    Western blot - Anti-Arc antibody [EPR18950] (ab183183)

    False colour image of Western blot: Anti-Arc antibody [EPR18950] staining at 1/500 dilution, shown in green; Mouse anti-GAPDH antibody [6C5] (Anti-GAPDH antibody [6C5] - Loading Control ab8245) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab183183 was shown to bind specifically to Arc. A band was observed at 45 kDa in wild-type Neuro-2a cell lysates with no signal observed at this size in arc knockout cell line Mouse ARC knockout Neuro-2a cell line ab280071 (knockout cell lysate Mouse ARC knockout Neuro-2a cell lysate ab280130). To generate this image, wild-type and arc knockout Neuro-2a cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in fluorescent western blot (TBS-based) blocking solution before incubation with primary antibodies overnight at 4°C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) at 1/20000 dilution.

    All lanes: Western blot - Anti-Arc antibody [EPR18950] (ab183183) at 1/500 dilution

    Lane 1: Wild-type Neuro-2a cell lysate at 20 µg

    Lane 2: ARC knockout Neuro-2a cell lysate at 20 µg

    Lane 2: Western blot - Mouse ARC knockout Neuro-2a cell line (Mouse ARC knockout Neuro-2a cell line ab280071)

    Lane 3: SH-SY5Y cell lysate at 20 µg

    Lane 4: K562 cell lysate at 20 µg

    Lane 5: Mouse Brain cell lysate at 20 µg

    Performed under reducing conditions.

    Predicted band size: 45 kDa

    Observed band size: 45 kDa

  • Western blot - Anti-Arc antibody [EPR18950] (ab183183), expandable thumbnail

    Western blot - Anti-Arc antibody [EPR18950] (ab183183)

    Blocking/Dilution buffer: 5% NFDM/TBST.

    Lanes 1 - 4: Western blot - Anti-Arc antibody [EPR18950] (ab183183) at 1/1000 dilution

    Lane 5: Western blot - Anti-Arc antibody [EPR18950] (ab183183) at 1/5000 dilution

    Lane 1: Mouse hippocampus lysate at 20 µg

    Lane 2: Rat hippocampus lysate at 20 µg

    Lane 3: Neuro-2a (mouse neuroblastoma cell line) whole cell lysate at 20 µg

    Lane 4: SH-SY5Y (human neuroblastoma cell line from bone marrow) whole cell lysate at 10 µg

    Lane 5: Human hippocampus lysate at 10 µg

    Secondary

    Lanes 1 - 4: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Lane 5: Western blot - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/4000 dilution

    Developed using the ECL technique.

    Predicted band size: 45 kDa

    Observed band size: 45 kDa

    Exposure time: 3min

  • Western blot - Anti-Arc antibody [EPR18950] (ab183183), expandable thumbnail

    Western blot - Anti-Arc antibody [EPR18950] (ab183183)

    Blocking/Dilution buffer: 5% NFDM/TBST.

    All lanes: Western blot - Anti-Arc antibody [EPR18950] (ab183183) at 1/1000 dilution

    Lane 1: Mouse brain lysate at 10 µg

    Lane 2: Mouse heart lysate at 10 µg

    Lane 3: Mouse kidney lysate at 10 µg

    Lane 4: Mouse spleen lysate at 10 µg

    Lane 5: Rat brain lysate at 10 µg

    Lane 6: Rat heart lysate at 10 µg

    Lane 7: Rat liver lysate at 10 µg

    Lane 8: Rat spleen lysate at 10 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Developed using the ECL technique.

    Predicted band size: 45 kDa

    Observed band size: 45 kDa

    Exposure time: 3min

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Arc antibody [EPR18950] (ab183183), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Arc antibody [EPR18950] (ab183183)

    Immunohistochemical analysis of paraffin-embedded mouse hippocampus tissue labeling Arc with ab183183 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Sparse nuclear staining (arrows) in the dentate gyrus of mouse hippocampus (PMID: 19750198, PMID: 19628007). Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunoprecipitation - Anti-Arc antibody [EPR18950] (ab183183), expandable thumbnail

    Immunoprecipitation - Anti-Arc antibody [EPR18950] (ab183183)

    Arc was immunoprecipitated from 0.35 mg of mouse brain lysate with ab183183 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab183183 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366), was used for detection at 1/1000 dilution.

    Lane 1: Mouse brain lysate 10 μg (Input).

    Lane 2: ab183183 IP in mouse brain lysate.

    Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab183183 in mouse brain lysate.

    Exposure time: 1 second

    Blocking/dilution buffer: 5% NFDM/TBST.

    All lanes: Immunoprecipitation - Anti-Arc antibody [EPR18950] (ab183183)

    Predicted band size: 42 kDa, 45 kDa, 75 kDa

    Observed band size: 42 kDa, 45 kDa, 75 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Arc antibody [EPR18950] (ab183183), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Arc antibody [EPR18950] (ab183183)

    Immunohistochemical analysis of paraffin-embedded mouse cerebral cortex tissue labeling Arc with ab183183 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Nuclear staining on neurons of mouse cerebral cortex. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

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