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AB234076

Anti-ARD1A antibody [3G3B9]

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(1 Publication)

Mouse Monoclonal ARD1A antibody. Suitable for Flow Cyt, WB, ICC/IF and reacts with Human, Mouse, African green monkey, Recombinant fragment - Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human NAA10 aa 100 to C-terminus.

View Alternative Names

ARD1, ARD1A, TE2, NAA10, N-alpha-acetyltransferase 10, N-terminal acetyltransferase complex ARD1 subunit homolog A, NatA catalytic subunit Naa10, hARD1

5 Images
Flow Cytometry - Anti-ARD1A antibody [3G3B9] (AB234076)
  • Flow Cyt

Supplier Data

Flow Cytometry - Anti-ARD1A antibody [3G3B9] (AB234076)

Flow Cytometry analysis of SMMC-7721 (human hepatocarcinoma cell line) cells, labeling ARD1A with ab234076 at a 1/200 dilution (green) compared to a negative control (red).

Immunocytochemistry/ Immunofluorescence - Anti-ARD1A antibody [3G3B9] (AB234076)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-ARD1A antibody [3G3B9] (AB234076)

HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for ARD1A (green) using ab234076 at 1/200 dilution in ICC/IF.

Blue : DRAQ5 fluorescent DNA dye. Red : Actin filaments have been labeled with AlexaFluor®555-Phalloidin.

Western blot - Anti-ARD1A antibody [3G3B9] (AB234076)
  • WB

Supplier Data

Western blot - Anti-ARD1A antibody [3G3B9] (AB234076)

All lanes:

Western blot - Anti-ARD1A antibody [3G3B9] (ab234076) at 1/100 dilution

Lane 1:

COS-7 (African green monkey kidney fibroblast-like cell line) cell lysate

Lane 2:

HEK-293 (human epithelial cell line from embryonic kidney) cell lysate

Lane 3:

HL-60 (human promyelocytic leukemia cell line) cell lysate

Lane 4:

MCF7 (human breast adenocarcinoma cell line) cell lysate

Lane 5:

HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate

Lane 6:

NIH-3T3 (mouse embryo fibroblast cell line) cell lysate

Lane 7:

C2C12 (mouse myoblast cell line) cell lysate

Predicted band size: 26 kDa

false

Western blot - Anti-ARD1A antibody [3G3B9] (AB234076)
  • WB

Supplier Data

Western blot - Anti-ARD1A antibody [3G3B9] (AB234076)

All lanes:

Western blot - Anti-ARD1A antibody [3G3B9] (ab234076) at 1/100 dilution

Lane 1:

HEK-293 (human epithelial cell line from embryonic kidney) cell lysate

Lane 2:

Human ARD1A (amino acids 111-235)-hIgGFc transfected HEH-293 cell lysate

Predicted band size: 26 kDa

false

Western blot - Anti-ARD1A antibody [3G3B9] (AB234076)
  • WB

Supplier Data

Western blot - Anti-ARD1A antibody [3G3B9] (AB234076)

Expected recombinant MW is 47.2 kDa

All lanes:

Western blot - Anti-ARD1A antibody [3G3B9] (ab234076) at 1/100 dilution

All lanes:

Recombinant human ARD1A (amino acids 111-235) protein

Predicted band size: 26 kDa

false

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

3G3B9

Isotype

IgG1

Carrier free

No

Reacts with

Mouse, Human, African green monkey

Applications

ICC/IF, WB, Flow Cyt

applications

Immunogen

Recombinant Fragment Protein within Human NAA10 aa 100 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P41227

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1/200 - 1/400", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/100 - 1/500", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/200 - 1/1000", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/100 - 1/500", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "African green monkey": { "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "1/200 - 1/400", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/100 - 1/500", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" }, "Recombinant fragment - Human": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/100 - 1/500", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Purification notes
Purified from tissue culture supernatant.
Storage buffer
Preservative: 0.05% Sodium azide Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ARD1A also known as NAA10 is a catalytic subunit of the N-terminal acetyltransferase complex. This protein has a mass of approximately 25 kDa. It acetylates the alpha-amino group of nascent polypeptides which affects stability localization and function of proteins. ARD1A is expressed in various tissues including heart skeletal muscle and liver. Its enzymatic activity requires a partnership with NAA15 another subunit which together form the N-terminal acetyltransferase A (NatA) complex.
Biological function summary

Acetylation by ARD1A modulates protein interactions and function. The NatA complex where ARD1A acts influences processes including cell cycle regulation and apoptosis. By modifying substrates critical for cell fate ARD1A plays a role in maintaining cellular balance. This complex helps in modifying proteins like actin which regulates the cytoskeleton a cellular structure essential for movement and division.

Pathways

Protein acetylation by ARD1A fits into the cellular signaling and protein degradation pathways. The complex alters substrates involved in proteasome-mediated degradation relating it to ubiquitin-proteasome pathways. Other interactions include the modulation of gene expression pathways impacting proteins like histones which are core to chromatin structure and gene regulation.

Improper ARD1A function can relate to cancer and neurodegenerative diseases. Alterations in ARD1A activity have connected it to tumorigenesis due to its role in cell growth regulation. Additionally decreased N-terminal acetylation by ARD1A has shown a link to neurodegeneration. This happens as it affects proteins such as tau which is significant in Alzheimer's disease when improperly modified.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Catalytic subunit of N-terminal acetyltransferase complexes which display alpha (N-terminal) acetyltransferase activity (PubMed : 15496142, PubMed : 19420222, PubMed : 19826488, PubMed : 20145209, PubMed : 20154145, PubMed : 25489052, PubMed : 27708256, PubMed : 29754825, PubMed : 32042062). Acetylates amino termini that are devoid of initiator methionine (PubMed : 19420222). The alpha (N-terminal) acetyltransferase activity may be important for vascular, hematopoietic and neuronal growth and development. Without NAA15, displays epsilon (internal) acetyltransferase activity towards HIF1A, thereby promoting its degradation (PubMed : 12464182). Represses MYLK kinase activity by acetylation, and thus represses tumor cell migration (PubMed : 19826488). Acetylates, and stabilizes TSC2, thereby repressing mTOR activity and suppressing cancer development (PubMed : 20145209). Acetylates HSPA1A and HSPA1B at 'Lys-77' which enhances its chaperone activity and leads to preferential binding to co-chaperone HOPX (PubMed : 27708256). Acetylates HIST1H4A (PubMed : 29754825). Acts as a negative regulator of sister chromatid cohesion during mitosis (PubMed : 27422821).
See full target information NAA10
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

International journal of nanomedicine 18:5407-5422 PubMed37753068

2023

Identification and Characterization of a Novel Nanobody Against Human CTGF to Reveal Its Antifibrotic Effect in an in vitro Model of Liver Fibrosis.

Applications

Unspecified application

Species

Unspecified reactive species

Rong Liu,Min Zhu,Jiaojiao Chen,Junwei Gai,Jing Huang,Yingqun Zhou,Yakun Wan,Chuantao Tu
View all publications
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