Rabbit Polyclonal ARD1A antibody. Suitable for WB, ICC/IF, IHC-P and reacts with Mouse, Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human NAA10 aa 1 to C-terminus.
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- eLife 10:2021compensates for in mice in the amino-terminal acetylation pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHyae Yon Kweon,Mi-Ni Lee,Max Dorfel,Seungwoon Seo,Leah Gottlieb,Thomas PaPazyan,Nina McTiernan,Rasmus Ree,David Bolton,Andrew Garcia,Michael Flory,Jonathan Crain,Alison Sebold,Scott Lyons,Ahmed Ismail,Elaine Marchi,Seong-Keun Sonn,Se-Jin Jeong,Sejin Jeon,Shinyeong Ju,Simon J Conway,Taesoo Kim,Hyun-Seok Kim,Cheolju Lee,Tae-Young Roh,Thomas Arnesen,Ronen Marmorstein,Goo Taeg Oh,Gholson J LyonPubMed 34355692
- Oncotarget 7:2709-202015microRNA-342-5p and miR-608 inhibit colon cancer tumorigenesis by targeting NAA10.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHongju Yang,Qian Li,Jie Niu,Bai Li,Dejun Jiang,Zhihua Wan,Qingmei Yang,Fei Jiang,Ping Wei,Song BaiPubMed 26646451
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 20% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human NAA10 aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link P41227
Reactivity data
Select an application| WB | ICC/IF | IHC-P | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
| Mouse | Tested | Expected | Expected |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/1000.00000 - 1/10000.00000 | Notes - |
Species Human | Dilution info 1/1000.00000 - 1/10000.00000 | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100.00000 - 1/1000.00000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100.00000 - 1/1000.00000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
Associated Products
Select an associated product type
1 product for Alternative Product
Target data
Function
Catalytic subunit of N-terminal acetyltransferase complexes which display alpha (N-terminal) acetyltransferase activity (PubMed:15496142, PubMed:19420222, PubMed:19826488, PubMed:20145209, PubMed:20154145, PubMed:25489052, PubMed:27708256, PubMed:29754825, PubMed:32042062). Acetylates amino termini that are devoid of initiator methionine (PubMed:19420222). The alpha (N-terminal) acetyltransferase activity may be important for vascular, hematopoietic and neuronal growth and development. Without NAA15, displays epsilon (internal) acetyltransferase activity towards HIF1A, thereby promoting its degradation (PubMed:12464182). Represses MYLK kinase activity by acetylation, and thus represses tumor cell migration (PubMed:19826488). Acetylates, and stabilizes TSC2, thereby repressing mTOR activity and suppressing cancer development (PubMed:20145209). Acetylates HSPA1A and HSPA1B at 'Lys-77' which enhances its chaperone activity and leads to preferential binding to co-chaperone HOPX (PubMed:27708256). Acetylates HIST1H4A (PubMed:29754825). Acts as a negative regulator of sister chromatid cohesion during mitosis (PubMed:27422821).
Alternative names
ARD1, ARD1A, TE2, NAA10, N-alpha-acetyltransferase 10, N-terminal acetyltransferase complex ARD1 subunit homolog A, NatA catalytic subunit Naa10, hARD1
Recommended products
Rabbit Polyclonal ARD1A antibody. Suitable for WB, ICC/IF, IHC-P and reacts with Mouse, Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human NAA10 aa 1 to C-terminus.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 20% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human NAA10 aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link P41227
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
ARD1A also known as NAA10 is a catalytic subunit of the N-terminal acetyltransferase complex. This protein has a mass of approximately 25 kDa. It acetylates the alpha-amino group of nascent polypeptides which affects stability localization and function of proteins. ARD1A is expressed in various tissues including heart skeletal muscle and liver. Its enzymatic activity requires a partnership with NAA15 another subunit which together form the N-terminal acetyltransferase A (NatA) complex.
- Biological function summary
Acetylation by ARD1A modulates protein interactions and function. The NatA complex where ARD1A acts influences processes including cell cycle regulation and apoptosis. By modifying substrates critical for cell fate ARD1A plays a role in maintaining cellular balance. This complex helps in modifying proteins like actin which regulates the cytoskeleton a cellular structure essential for movement and division.
- Pathways
Protein acetylation by ARD1A fits into the cellular signaling and protein degradation pathways. The complex alters substrates involved in proteasome-mediated degradation relating it to ubiquitin-proteasome pathways. Other interactions include the modulation of gene expression pathways impacting proteins like histones which are core to chromatin structure and gene regulation.
- Associated diseases and disorders
Improper ARD1A function can relate to cancer and neurodegenerative diseases. Alterations in ARD1A activity have connected it to tumorigenesis due to its role in cell growth regulation. Additionally decreased N-terminal acetylation by ARD1A has shown a link to neurodegeneration. This happens as it affects proteins such as tau which is significant in Alzheimer's disease when improperly modified.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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4 product images
Western blot - Anti-ARD1A antibody (ab155687)
12% SDS PAGE
All lanes: Western blot - Anti-ARD1A antibody (ab155687) at 1/5000 dilution
Lane 1: H1299 cell lysate, transfected with scramble siRNA at 30 µg
Lane 2: H1299 cell lysate, transfected with ARDIA siRNA at 30 µg
Predicted band size: 26 kDa
Western blot - Anti-ARD1A antibody (ab155687)
12% SDS PAGE
All lanes: Western blot - Anti-ARD1A antibody (ab155687) at 1/1000 dilution
Lane 1: NIH 3T3 whole cell lysate at 30 µg
Lane 2: JC whole cell lysate at 30 µg
Lane 3: BCL1 whole cell lysate at 30 µg
Lane 4: C2C12 whole cell lysate at 30 µg
Lane 5: RAW264.7 whole cell lysate at 30 µg
Predicted band size: 26 kDa
Immunocytochemistry/ Immunofluorescence - Anti-ARD1A antibody (ab155687)
Immunofluorescent analysis of methanol-fixed HeLa cells (100% for 5 mins) labeling ARD1A with ab155687 at 1/500 dilution. Lower image shows cells co-stained with Hoechst 33342.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARD1A antibody (ab155687)
Immunohistochemical analysis of paraffin-embedded Human PC13 xenograft tissue labeling ARD1A with ab155687 at 1/500 dilution.
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