JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB133768

Anti-ARF GAP 2 antibody [EPR8497]

Be the first to review this product! Submit a review

|

(3 Publications)

Rabbit Recombinant Monoclonal ARF GAP 2 antibody. Suitable for IHC-P, WB and reacts with Human, Mouse, Rat samples. Cited in 3 publications.

View Alternative Names

ZNF289, Nbla10535, ARFGAP2, ADP-ribosylation factor GTPase-activating protein 2, ARF GAP 2, GTPase-activating protein ZNF289, Zinc finger protein 289

4 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARF GAP 2 antibody [EPR8497] (AB133768)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARF GAP 2 antibody [EPR8497] (AB133768)

Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue labelling ARF GAP 2 with ab133768 at 1/50 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARF GAP 2 antibody [EPR8497] (AB133768)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARF GAP 2 antibody [EPR8497] (AB133768)

Immunohistochemical analysis of paraffin-embedded Human colon tissue labelling ARF GAP 2 with ab133768 at 1/50 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Western blot - Anti-ARF GAP 2 antibody [EPR8497] (AB133768)
  • WB

Unknown

Western blot - Anti-ARF GAP 2 antibody [EPR8497] (AB133768)

All lanes:

Western blot - Anti-ARF GAP 2 antibody [EPR8497] (ab133768) at 1/1000 dilution

Lane 1:

Human fetal liver tissue lysate at 10 µg

Lane 2:

Human skeletal muscle tissue lysate at 10 µg

Lane 3:

HepG2 cell lysate at 10 µg

Lane 4:

Human fetal heart tissue lysate at 10 µg

Lane 5:

HeLa cell lysate at 10 µg

Secondary

All lanes:

HRP labelled Goat anti Rabbit IgG at 1/2000 dilution

Predicted band size: 56 kDa

false

OI-RD Scanning - Anti-ARF GAP 2 antibody [EPR8497] (AB133768)
  • OI-RD Scanning

Unknown

OI-RD Scanning - Anti-ARF GAP 2 antibody [EPR8497] (AB133768)

We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody. Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

  • Carrier free

    Anti-ARF GAP 2 antibody [EPR8497] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR8497

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style
left-column

Complementary Products

Primary Antibodies

AB246865

Anti-Apolipoprotein O antibody

Immunocytochemistry/ Immunofluorescence - Anti-Apolipoprotein O antibody (AB246865)

  • 0
  • 0
Primary Antibodies

AB273050

Anti-CCR2 antibody [EPR20844-15]

20ul selling size|BOND RX™ Validated|RabMAb|Recombinant|KO Validated|Trial Size

Flow Cytometry - Anti-CCR2 antibody [EPR20844-15] (AB273050)

  • 4
  • 2
Primary Antibodies

AB89117

Anti-SLC25A3/PHC antibody

Immunocytochemistry/ Immunofluorescence - Anti-SLC25A3/PHC antibody (AB89117)

  • 0
  • 0
Primary Antibodies

AB180149

Anti-ATP5G1/G2/G3 antibody [EPR13908]

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP5G1/G2/G3 antibody [EPR13908] (AB180149)

  • 0
  • 0
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/100", "IHCP-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" } } }
style
left-column

Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Conditional Ambient
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Stable for 12 months at -20°C
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ARF GAP 2 also known as ADP-ribosylation factor GTPase-activating protein 2 acts as a GAP protein that enhances the intrinsic GTPase activity of ARF proteins. This action helps modulate the ARF proteins' ability to switch between active and inactive forms which plays a role in their regulatory functions. ARF GAP 2 possesses a molecular weight of approximately 48 kDa and shows expression in various tissues particularly in brain heart and skeletal muscle tissues.
Biological function summary

ARF GAP 2 participates in the processes controlling vesicular trafficking and cytoskeletal dynamics. It interacts within a complex that manages the assembly and disassembly of actin structures which are important for cellular movement and shape changes. By influencing the ARF proteins it contributes to membrane trafficking and protein sorting essential for maintaining cellular organization and function.

Pathways

ARF GAP 2 integrates into the Arf-related signaling route and the endocytic pathway. Its regulatory role ties closely with the ARF6 protein another small GTPase that influences endocytosis and actin reorganization. Through its interaction in these pathways ARF GAP 2 assists in the coordination of membrane compartmentalization and dynamic changes.

ARF GAP 2 has associations with neurological and cardiovascular conditions. Its role in regulating actin structures and vesicular trafficking implicates it in disease pathways connected to protein mistrafficking and cellular dysfunction. In Alzheimer’s disease interactions with Tau protein point to a possible contribution in neurodegeneration. Similarly in cardiovascular disease its relationship with ARF6 suggests involvement in endothelial dysfunction and aortic aneurysm formation.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

GTPase-activating protein (GAP) for ADP ribosylation factor 1 (ARF1). Implicated in coatomer-mediated protein transport between the Golgi complex and the endoplasmic reticulum. Hydrolysis of ARF1-bound GTP may lead to dissociation of coatomer from Golgi-derived membranes to allow fusion with target membranes.
See full target information ARFGAP2
style
left-column

Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Food science & nutrition 13:e70464 PubMed40535916

2025

Ginsenoside Rh1 Alleviates Allergic Rhinitis by Mediating Mitochondrial Autophagy via Activation of the AMPK/ULK1/FUNDC1 Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Jiangang Wang,Yalin Zhang,Jingmei Chai,Jianing Yang,Longzhu Dai,Yi Yang,Yulian Zhang,Yongde Jin,Chongyang Wang,Guanghai Yan

Communications biology 4:1370 PubMed34876695

2021

Tankyrase-1-mediated degradation of Golgin45 regulates glycosyltransferase trafficking and protein glycosylation in Rab2-GTP-dependent manner.

Applications

Unspecified application

Species

Unspecified reactive species

Xihua Yue,Neeraj Tiwari,Lianhui Zhu,Hai Dang Truong Ngo,Jae-Min Lim,Bopil Gim,Shuaiyang Jing,Yijing Wang,Yi Qian,Intaek Lee

BMC biology 19:194 PubMed34493279

2021

ACBD3 modulates KDEL receptor interaction with PKA for its trafficking via tubulovesicular carrier.

Applications

Unspecified application

Species

Unspecified reactive species

Xihua Yue,Yi Qian,Lianhui Zhu,Bopil Gim,Mengjing Bao,Jie Jia,Shuaiyang Jing,Yijing Wang,Chuanting Tan,Francesca Bottanelli,Pascal Ziltener,Sunkyu Choi,Piliang Hao,Intaek Lee
View all publications
style
left-column
style
left-column
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com