Rabbit Polyclonal ARF1 antibody. Suitable for IHC-P, IP, WB, ICC/IF and reacts with Mouse, Human, Rat, Dog samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human ARF1 aa 150 to C-terminus.
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- Frontiers in pharmacology 13:10093802022The olfactory receptor OR51E2 activates ERK1/2 through the Golgi-localized Gβγ-PI3Kγ-ARF1 pathway in prostate cancer cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXin Xu,Mostafa Khater,Guangyu WuPubMed 36313302
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Preservative: 0.05% Sodium azide
Constituents: 69% PBS, 30% Glycerol (glycerin, glycerine), 0.1% BSA- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human ARF1 aa 150 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link P84077
Reactivity data
Select an application| IHC-P | IP | WB | ICC/IF | |
|---|---|---|---|---|
| Human | Tested | Tested | Tested | Tested |
| Mouse | Tested | Expected | Tested | Expected |
| Rat | Expected | Expected | Tested | Expected |
| Cow | Predicted | Predicted | Predicted | Predicted |
| Dog | Expected | Expected | Tested | Expected |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/10.00000 - 1/100.00000 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Species Human | Dilution info 1/10.00000 - 1/100.00000 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Dog | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Cow | Dilution info - | Notes - |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 2 µg/mg of lysate | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Dog | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Cow | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/3000 | Notes - |
Species Rat | Dilution info 1/3000 | Notes - |
Species Dog | Dilution info 1/3000 | Notes - |
Species Mouse | Dilution info 1/3000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Cow | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 5 µg/mL | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Dog | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Cow | Dilution info - | Notes - |
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Target data
Function
Small GTPase involved in protein trafficking between different compartments (PubMed:8253837). Modulates vesicle budding and uncoating within the Golgi complex (PubMed:8253837). In its GTP-bound form, triggers the recruitment of coatomer proteins to the Golgi membrane (PubMed:8253837). The hydrolysis of ARF1-bound GTP, which is mediated by ARFGAPs proteins, is required for dissociation of coat proteins from Golgi membranes and vesicles (PubMed:8253837). The GTP-bound form interacts with PICK1 to limit PICK1-mediated inhibition of Arp2/3 complex activity; the function is linked to AMPA receptor (AMPAR) trafficking, regulation of synaptic plasticity of excitatory synapses and spine shrinkage during long-term depression (LTD) (By similarity). Plays a key role in the regulation of intestinal stem cells and gut microbiota, and is essential for maintaining intestinal homeostasis (By similarity). Plays also a critical role in mast cell expansion but not in mast cell maturation by facilitating optimal mTORC1 activation (By similarity). (Microbial infection) Functions as an allosteric activator of the cholera toxin catalytic subunit, an ADP-ribosyltransferase.
Alternative names
ADP-ribosylation factor 1, ARF1
Recommended products
Rabbit Polyclonal ARF1 antibody. Suitable for IHC-P, IP, WB, ICC/IF and reacts with Mouse, Human, Rat, Dog samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human ARF1 aa 150 to C-terminus.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Preservative: 0.05% Sodium azide
Constituents: 69% PBS, 30% Glycerol (glycerin, glycerine), 0.1% BSA- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human ARF1 aa 150 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link P84077
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
ARF1 also known as ADP-ribosylation factor 1 or ARF1 protein is a small GTPase with a molecular mass of approximately 20 kDa. Commonly expressed in a variety of cell types ARF1 plays a central role in vesicle trafficking. As an activator ARF1 interacts with different GTPase-activating proteins such as ARFGAP1 and BIG1 ensuring the proper distribution of proteins and lipids in cells. ARF1's expression occurs across numerous cellular compartments including the Golgi apparatus aiding in the formation of transport vesicles.
- Biological function summary
ARF1 is essential in regulating membrane dynamics and vesicular traffic. It forms part of the COPI and clathrin-coated vesicle complexes where it recruits coat proteins to budding vesicles. This recruitment is fundamental for maintaining Golgi structure and function. Additionally ARF1 plays a role in cytokinesis by interacting with the septin cytoskeleton. The protein also influences actin cytoskeleton remodeling which is pivotal for cell shape changes and motility.
- Pathways
ARF1 is central to both the endocytic and secretory pathways. It collaborates with proteins like ARFS and 3F1 in modulating the trafficking of cargo between the endoplasmic reticulum and Golgi. Within the secretory pathway ARF1 interacts with SNARE proteins to facilitate vesicle docking and fusion. Its actions in pathways maintain cellular homeostasis and promote proper cellular response to various stimuli.
- Associated diseases and disorders
ARF1 has links to cancer and Alzheimer's disease. Overexpression of ARF1 correlates with tumor progression and metastasis impacting cell proliferation and survival mechanisms. Additionally its disruption associates with Alzheimer's where it may influence amyloid precursor protein processing alongside interactions with GAP proteins. Understanding ARF1's role in these conditions highlights its potential as a therapeutic target stressing the importance of studying its interactions with disease-related proteins.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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9 product images
Western blot - Anti-ARF1 antibody (ab183576)
Lanes 1 - 4: Merged signal (red and green). Green - ab183576 observed at 18 kDa. Red - loading control, Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 (Mouse anti-Alpha Tubulin [DM1A]) observed at 55kDa.
ab183576 was shown to react with ARF1 in wild-type HeLa cells in Western blot with loss of signal observed in ARF1 knockout cell line Human ARF1 knockout HeLa cell line ab264939 (ARF1 knockout cell lysate Human ARF1 knockout HeLa cell lysate ab257353). Wild-type HeLa and ARF1 knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3 % milk in TBS-T (0.1 % Tween®) before incubation with ab183576 and Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 (Mouse anti-Alpha Tubulin [DM1A]) overnight at 4 °C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1 in 20000 dilution for 1 h at room temperature before imaging.All lanes: Western blot - Anti-ARF1 antibody (ab183576) at 1/1000 dilution
Lane 1: Wild-type HeLa cell lysate at 20 µg
Lane 2: ARF1 knockout HeLa cell lysate at 20 µg
Lane 2: Western blot - Human ARF1 knockout HeLa cell line (Human ARF1 knockout HeLa cell line ab264939)
Lane 3: MDA-MB-231 cell lysate at 20 µg
Lane 4: PANC-1 cell lysate at 20 µg
Performed under reducing conditions.
Predicted band size: 21 kDa
Observed band size: 18 kDa
Western blot - Anti-ARF1 antibody (ab183576)
All lanes: Western blot - Anti-ARF1 antibody (ab183576) at 1/3000 dilution
Lane 1: MDA-MB-231 whole cell extract (human breast adenocarcinoma cell line) at 30 µg
Lane 2: MDA-MB-231 whole cell extract (human breast adenocarcinoma cell line) treated with 5ug/ml Brefeldin for 60 minutes at 30 µg
Lane 3: HeLa whole cell extract at 30 µg
Lane 4: U2 OS whole cell extract (human bone osteosarcoma epithelial cell line) at 30 µg
Lane 5: DU 145 whole cell extract (Human prostate carcinoma cell line) at 30 µg
Lane 6: PC-3 whole cell extract (human prostate cancer cell line) at 30 µg
Lane 7: SK-OV-3 whole cell extract (Human ovarian cancer cell line) at 30 µg
SecondaryAll lanes: Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate at 1/4000 dilution
Predicted band size: 21 kDa
Immunocytochemistry/ Immunofluorescence - Anti-ARF1 antibody (ab183576)
Immunocytochemistry analysis of 4% paraformaldehyde-fixed 0.1% Triton™ X-100 permeabilized MDA-MB-231 cells staining ARF1 with ab183576 at 5μg/ml, and Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate at 1/2000 dilution (green). Nuclear counterstain was ProLong™ Diamond Antifade Mountant with DAPI (blue), and F-actin was stained with Rhodamine Phalloidin (red). Negative control used no primary antibody.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARF1 antibody (ab183576)
Immunohistochemistry analysis of paraffin-embedded human mouse colon tissue staining ARF1 with ab183576 at 1/100 dilution (right), and negative control (left) with no primary antibody. Atigen retrieval method using sodium citrate pH6, and detection was with an HRP-conjugated secondary antibody.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARF1 antibody (ab183576)
Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue staining ARF1 with ab183576 at 1/100 dilution (right), and negative control (left) with no primary antibody. Atigen retrieval method using sodium citrate pH6, and detection was with an HRP-conjugated secondary antibody.
Immunoprecipitation - Anti-ARF1 antibody (ab183576)
Detection of ARF1 in Immunoprecipitates of HeLa cell lysate. Antigen-antibody complexes were formed by incubating 500μg of HeLa whole cell lysate with 2μg ab183576 (lane 2) compared with HeLa cell lysate as a positive control (lane 1). For detection, ab183576 was used at 1/1000 dilution.
All lanes: Immunoprecipitation - Anti-ARF1 antibody (ab183576)
Developed using the ECL technique.
Predicted band size: 21 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARF1 antibody (ab183576)
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue staining ARF1 with ab183576 at 1/100 dilution (right), and negative control (left) with no primary antibody. Atigen retrieval method using sodium citrate pH6, and detection was with an HRP-conjugated secondary antibody.
Western blot - Anti-ARF1 antibody (ab183576)
All lanes: Western blot - Anti-ARF1 antibody (ab183576) at 1/1000 dilution
Lane 1: HeLa cell lysate at 25 µg
Lane 2: U2 OS cell lysate at 25 µg
Lane 3: 3T3 cell lysate at 25 µg
Lane 4: NRK cell lysate at 25 µg
Lane 5: MDCK cell lysate at 25 µg
SecondaryAll lanes: goat anti-rabbit IgG-HRP at 1/1500 dilution
Developed using the ECL technique.
Predicted band size: 21 kDa
Immunocytochemistry/ Immunofluorescence - Anti-ARF1 antibody (ab183576)
Immunofluorescent analysis of HeLa cells (formalin-fixed, 0.1% Triton X-100 permeabilized) labeling ARF1 with ab183576 at 1/100 dilution followed with DyLight 488 goat anti-rabbit IgG secondary antibody at 1/400 dilution. Nuclei (blue) were stained with Hoechst 33342 dye.
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