Mouse Monoclonal ARF1 antibody. Suitable for IHC-P and reacts with Human samples. Cited in 9 publications. Immunogen corresponding to Synthetic Peptide within Human ARF1 aa 150 to C-terminus.
Images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARF1 antibody [ARFS 3F1] (AB11038), expandable thumbnail](https://content.abcam.com/products/images/arf1-antibody-arfs-3f1-ab11038--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img8189.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARF1 antibody [ARFS 3F1] (AB11038)")
Publications
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- Gastroenterology 163:1064-1078.e102022Malondialdehyde-Acetaldehyde Extracellular Matrix Protein Adducts Attenuate Unfolded Protein Response During Alcohol and Smoking-Induced Pancreatitis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRakesh Bhatia,Christopher M Thompson,Emalie J Clement,Koelina Ganguly,Jesse L Cox,Sanchita Rauth,Jawed Akhtar Siddiqui,Simran S Mashiana,Maneesh Jain,Todd A Wyatt,Harmeet S Mashiana,Shailender Singh,Nicholas T Woods,Kusum K Kharbanda,Surinder K Batra,Sushil KumarPubMed 35788346
- Molecular biology of the cell 31:27-442019Distinct anterograde trafficking pathways of BACE1 and amyloid precursor protein from the TGN and the regulation of amyloid-β production.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJing Zhi A Tan,Lou Fourriere,Jingqi Wang,Franck Perez,Gaelle Boncompain,Paul A GleesonPubMed 31746668
- Journal of cellular biochemistry 121:344-3522019LncRNA TP73-AS1 is a novel regulator in cervical cancer via miR-329-3p/ARF1 axis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJingning Xu,Jinmei ZhangPubMed 31232491
- Journal of immunology (Baltimore, Md. : 1950) 191:1496-5042013Chemokine unresponsiveness of chronic lymphocytic leukemia cells results from impaired endosomal recycling of Rap1 and is associated with a distinctive type of immunological anergy.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDerek S Pye et. al.PubMed 23804711
- Genes & development 24:1496-5062010The CNK1 scaffold binds cytohesins and promotes insulin pathway signaling.Applications:WBReactive species:HumanJunghwa Lim,Ming Zhou,Timothy D Veenstra,Deborah K MorrisonPubMed 20634316
- Traffic (Copenhagen, Denmark) 9:798-8122008Adaptor protein Ruk/CIN85 is associated with a subset of COPI-coated membranes of the Golgi complex.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSerhiy Havrylov et. al.PubMed 18266907
- The Journal of biological chemistry 283:6085-942007Molecular mechanism of mitotic Golgi disassembly and reassembly revealed by a defined reconstitution assay.Applications:WBReactive species:Unspecified reactive speciesDanming Tang,Kari Mar,Graham Warren,Yanzhuang WangPubMed 18156178
- Molecular biology of the cell 19:822-322007Fbx8 makes Arf6 refractory to function via ubiquitination.
- Molecular biology of the cell 18:501-112006Endogenous ARF6 interacts with Rac1 upon angiotensin II stimulation to regulate membrane ruffling and cell migration.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMathieu Cotton,Pierre-Luc Boulay,Tanguy Houndolo,Nicolas Vitale,Julie A Pitcher,Audrey ClaingPubMed 17122362
Key facts
- Isotype
- IgG2a
- Host species
- Mouse
- Storage buffer
Preservative: 0.02% Sodium azide
Constituents: 99.98% PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- Synthetic Peptide within Human ARF1 aa 150 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link P84077
Reactivity data
Select an application| IHC-P | |
|---|---|
| Human | Tested |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1 µg/mL | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Associated Products
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Target data
Function
Small GTPase involved in protein trafficking between different compartments (PubMed:8253837). Modulates vesicle budding and uncoating within the Golgi complex (PubMed:8253837). In its GTP-bound form, triggers the recruitment of coatomer proteins to the Golgi membrane (PubMed:8253837). The hydrolysis of ARF1-bound GTP, which is mediated by ARFGAPs proteins, is required for dissociation of coat proteins from Golgi membranes and vesicles (PubMed:8253837). The GTP-bound form interacts with PICK1 to limit PICK1-mediated inhibition of Arp2/3 complex activity; the function is linked to AMPA receptor (AMPAR) trafficking, regulation of synaptic plasticity of excitatory synapses and spine shrinkage during long-term depression (LTD) (By similarity). Plays a key role in the regulation of intestinal stem cells and gut microbiota, and is essential for maintaining intestinal homeostasis (By similarity). Plays also a critical role in mast cell expansion but not in mast cell maturation by facilitating optimal mTORC1 activation (By similarity). (Microbial infection) Functions as an allosteric activator of the cholera toxin catalytic subunit, an ADP-ribosyltransferase.
Alternative names
ADP-ribosylation factor 1, ARF1
Recommended products
Mouse Monoclonal ARF1 antibody. Suitable for IHC-P and reacts with Human samples. Cited in 9 publications. Immunogen corresponding to Synthetic Peptide within Human ARF1 aa 150 to C-terminus.
Key facts
- Isotype
- IgG2a
- Host species
- Mouse
- Storage buffer
Preservative: 0.02% Sodium azide
Constituents: 99.98% PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- Synthetic Peptide within Human ARF1 aa 150 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link P84077
- Clone number
- ARFS 3F1
- Purification technique
- Affinity purification Protein A
- Specificity
This antibody is specific to class I ARF.
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
ARF1 also known as ADP-ribosylation factor 1 or ARF1 protein is a small GTPase with a molecular mass of approximately 20 kDa. Commonly expressed in a variety of cell types ARF1 plays a central role in vesicle trafficking. As an activator ARF1 interacts with different GTPase-activating proteins such as ARFGAP1 and BIG1 ensuring the proper distribution of proteins and lipids in cells. ARF1's expression occurs across numerous cellular compartments including the Golgi apparatus aiding in the formation of transport vesicles.
- Biological function summary
ARF1 is essential in regulating membrane dynamics and vesicular traffic. It forms part of the COPI and clathrin-coated vesicle complexes where it recruits coat proteins to budding vesicles. This recruitment is fundamental for maintaining Golgi structure and function. Additionally ARF1 plays a role in cytokinesis by interacting with the septin cytoskeleton. The protein also influences actin cytoskeleton remodeling which is pivotal for cell shape changes and motility.
- Pathways
ARF1 is central to both the endocytic and secretory pathways. It collaborates with proteins like ARFS and 3F1 in modulating the trafficking of cargo between the endoplasmic reticulum and Golgi. Within the secretory pathway ARF1 interacts with SNARE proteins to facilitate vesicle docking and fusion. Its actions in pathways maintain cellular homeostasis and promote proper cellular response to various stimuli.
- Associated diseases and disorders
ARF1 has links to cancer and Alzheimer's disease. Overexpression of ARF1 correlates with tumor progression and metastasis impacting cell proliferation and survival mechanisms. Additionally its disruption associates with Alzheimer's where it may influence amyloid precursor protein processing alongside interactions with GAP proteins. Understanding ARF1's role in these conditions highlights its potential as a therapeutic target stressing the importance of studying its interactions with disease-related proteins.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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1 product image
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARF1 antibody [ARFS 3F1] (ab11038), expandable thumbnail](https://content.abcam.com/products/images/arf1-antibody-arfs-3f1-ab11038--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img8189.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARF1 antibody [ARFS 3F1] (ab11038)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARF1 antibody [ARFS 3F1] (ab11038)
IHC image of ab11038 staining in human normal liver formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab11038, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
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