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Rabbit Recombinant Monoclonal ARF1 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human, Recombinant full length protein - Human samples. Cited in 7 publications.

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Images

Western blot - Anti-ARF1 + ARF3 antibody [EPR443] (AB108347), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-ARF1 + ARF3 antibody [EPR443] (AB108347), expandable thumbnail
  • Western blot - Anti-ARF1 + ARF3 antibody [EPR443] (AB108347), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARF1 + ARF3 antibody [EPR443] (AB108347), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARF1 + ARF3 antibody [EPR443] (AB108347), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PWBICC/IFIPFlow Cyt
Human
Tested
Tested
Tested
Not recommended
Not recommended
Mouse
Predicted
Predicted
Predicted
Not recommended
Not recommended
Rat
Predicted
Predicted
Predicted
Not recommended
Not recommended
Recombinant full length protein - Human
Not recommended
Tested
Not recommended
Not recommended
Not recommended

Tested
Tested

Species
Human
Dilution info
1/100 - 1/250
Notes

Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.

Predicted
Predicted

Species
Mouse, Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Recombinant full length protein - Human
Dilution info
-
Notes

-

Tested
Tested

Species
Recombinant full length protein - Human
Dilution info
1/1000 - 1/10000
Notes

-

Species
Human
Dilution info
1/1000 - 1/10000
Notes

-

Predicted
Predicted

Species
Mouse, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/50 - 1/100
Notes

-

Predicted
Predicted

Species
Mouse, Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Recombinant full length protein - Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Rat, Recombinant full length protein - Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Rat, Recombinant full length protein - Human
Dilution info
-
Notes

-

Associated Products

Select an associated product type

2 products for Alternative Product

Target data

Function

Small GTPase involved in protein trafficking between different compartments (PubMed:8253837). Modulates vesicle budding and uncoating within the Golgi complex (PubMed:8253837). In its GTP-bound form, triggers the recruitment of coatomer proteins to the Golgi membrane (PubMed:8253837). The hydrolysis of ARF1-bound GTP, which is mediated by ARFGAPs proteins, is required for dissociation of coat proteins from Golgi membranes and vesicles (PubMed:8253837). The GTP-bound form interacts with PICK1 to limit PICK1-mediated inhibition of Arp2/3 complex activity; the function is linked to AMPA receptor (AMPAR) trafficking, regulation of synaptic plasticity of excitatory synapses and spine shrinkage during long-term depression (LTD) (By similarity). Plays a key role in the regulation of intestinal stem cells and gut microbiota, and is essential for maintaining intestinal homeostasis (By similarity). Plays also a critical role in mast cell expansion but not in mast cell maturation by facilitating optimal mTORC1 activation (By similarity). (Microbial infection) Functions as an allosteric activator of the cholera toxin catalytic subunit, an ADP-ribosyltransferase.

Additional Targets

ADP-ribosylation factor 3

Alternative names

ADP-ribosylation factor 1, ARF1

Recommended products

Rabbit Recombinant Monoclonal ARF1 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human, Recombinant full length protein - Human samples. Cited in 7 publications.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR443
Purification technique
Affinity purification Protein A
Specificity

The immunogen used to raise this antibody shares 100% homology with human ARF3, 96% homology with ARF4 and ARF5, and 88% homology with ARF6. Cross-reactivity with the latter three proteins have not been confirmed experimentally.

Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Stable for 12 months at -20°C

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

ARF1 and ARF3 also known as ADP-ribosylation factors 1 and 3 are small GTP-binding proteins belonging to the ARF family. ARF1 has a molecular mass of approximately 21 kDa. These proteins are expressed in most eukaryotic cells where they play roles in vesicular trafficking processes. ARF1 is mainly located in the Golgi apparatus while ARF3 appears in the plasma membrane and cytoplasm. They function as molecular switches by cycling between active GTP-bound and inactive GDP-bound states which regulates the formation and budding of vesicles.

Biological function summary

ARF1 and ARF3 are significant in the intracellular trafficking of proteins and lipids. The proteins are part of the coatomer protein complex (COPI) important for retrograde transport from the Golgi to the endoplasmic reticulum. By recruiting coat proteins to the Golgi membranes ARF1 and ARF3 facilitate the sorting and packaging of cargo molecules into transport vesicles. This process sustains the maintenance of organelle identity and the dynamics of membrane remodeling.

Pathways

ARF1 and ARF3 are involved in key cellular pathways particularly those related to vesicular trafficking and membrane dynamics. They participate in the COPI and clathrin-mediated pathways. Through these pathways ARF1 interacts with proteins like PDZD8 while ARF3 works with proteins involved in primary cilia formation. These interactions underline their integrative role in maintaining proper cellular organization and communication.

Associated diseases and disorders

ARF1 and ARF3 have links to cancer and neurodegenerative diseases. Dysregulation of ARF1 is associated with various cancer types due to its role in cell proliferation and migration. ARF3 has connections to neurodegenerative disorders where altered vesicular trafficking can lead to pathological conditions. ARF1's interaction with the protein NFDM-442 highlights its importance in disease contexts whereby changes in these interactions might contribute to disease progression and severity.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

5 product images

  • Western blot - Anti-ARF1 + ARF3 antibody [EPR443] (ab108347), expandable thumbnail

    Western blot - Anti-ARF1 + ARF3 antibody [EPR443] (ab108347)

    Blocking and diluting buffer: 5% NFDM /TBST

    Human ARF-1 recombinant protein, (Cat#:ab123538 )
    Human ARF-3 recombinant protein, (Cat#:Recombinant Human ARF3 protein ab113581 )

    All lanes: Western blot - Anti-ARF1 + ARF3 antibody [EPR443] (ab108347) at 1/50000 dilution

    Lane 1: Full length human ARF-1 recombinant protein at 0.015 µg

    Lane 2: Full length human ARF-3 recombinant protein at 0.015 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Predicted band size: 21 kDa

    Observed band size: 23 kDa

    Exposure time: 3min

  • Immunocytochemistry/ Immunofluorescence - Anti-ARF1 + ARF3 antibody [EPR443] (ab108347), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-ARF1 + ARF3 antibody [EPR443] (ab108347)

    ICC/IF image of ab108347 stained CaCo2 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab108347 at 1/100 dilution overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (Goat Anti-Rabbit IgG H&L (DyLight® 488) preadsorbed ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43μM.

  • Western blot - Anti-ARF1 + ARF3 antibody [EPR443] (ab108347), expandable thumbnail

    Western blot - Anti-ARF1 + ARF3 antibody [EPR443] (ab108347)

    All lanes: Western blot - Anti-ARF1 + ARF3 antibody [EPR443] (ab108347) at 1/1000 dilution

    Lane 1: Human fetal kidney tissue lysate at 10 µg

    Lane 2: Human fetal brain tissue lysate at 10 µg

    Lane 3: MCF7 cell lysate at 10 µg

    Predicted band size: 21 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARF1 + ARF3 antibody [EPR443] (ab108347), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARF1 + ARF3 antibody [EPR443] (ab108347)

    Immunohistochemical analysis of ARF1 in paraffin embedded Human breast ductal carcinoma tissue, using ab108347 at a dilution of 1/100.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARF1 + ARF3 antibody [EPR443] (ab108347), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARF1 + ARF3 antibody [EPR443] (ab108347)

    Immunohistochemical analysis of ARF1 in paraffin embedded Human colonic adenocarcinoma tissue, using ab108347 at a dilution of 1/100.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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