AB108347

Anti-ARF1 + ARF3 antibody [EPR443]

RabMAb
Recombinant
What is this?

Be the first to review this product! Submit a review

(7 Publications)

Rabbit Recombinant Monoclonal ARF1 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human, Recombinant full length protein - Human samples. Cited in 7 publications.

View Alternative Names

ADP-ribosylation factor 1, ARF1, ADP-ribosylation factor 3, ARF3

5 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARF1 + ARF3 antibody [EPR443] (AB108347)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARF1 + ARF3 antibody [EPR443] (AB108347)

Immunohistochemical analysis of ARF1 in paraffin embedded Human colonic adenocarcinoma tissue using ab108347 at a dilution of 1/100.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Immunocytochemistry/ Immunofluorescence - Anti-ARF1 + ARF3 antibody [EPR443] (AB108347)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-ARF1 + ARF3 antibody [EPR443] (AB108347)

ICC/IF image of ab108347 stained CaCo2 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab108347 at 1/100 dilution overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43μM.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARF1 + ARF3 antibody [EPR443] (AB108347)

Immunohistochemical analysis of ARF1 in paraffin embedded Human breast ductal carcinoma tissue using ab108347 at a dilution of 1/100.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Lab

Western blot - Anti-ARF1 + ARF3 antibody [EPR443] (AB108347)

Blocking and diluting buffer : 5% NFDM /TBST

Human ARF-1 recombinant protein, (Cat# : ab123538 )
Human ARF-3 recombinant protein, (Cat# : ab113581 )

All lanes:

Western blot - Anti-ARF1 + ARF3 antibody [EPR443] (ab108347) at 1/50000 dilution

Lane 1:

Full length human ARF-1 recombinant protein at 0.015 µg

Lane 2:

Full length human ARF-3 recombinant protein at 0.015 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 21 kDa

Observed band size: 23 kDa

false

Exposure time: 3min

Unknown

Western blot - Anti-ARF1 + ARF3 antibody [EPR443] (AB108347)

All lanes:

Western blot - Anti-ARF1 + ARF3 antibody [EPR443] (ab108347) at 1/1000 dilution

Lane 1:

Human fetal kidney tissue lysate at 10 µg

Lane 2:

Human fetal brain tissue lysate at 10 µg

Lane 3:

MCF7 cell lysate at 10 µg

Predicted band size: 21 kDa

false

665 Alexa Fluor® 647

Alexa Fluor® 647 Anti-ARF1 + ARF3 antibody [EPR443]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR443

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, ICC/IF, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

The immunogen used to raise this antibody shares 100% homology with human ARF3, 96% homology with ARF4 and ARF5, and 88% homology with ARF6. Cross-reactivity with the latter three proteins have not been confirmed experimentally.

style

left-column

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/250", "IHCP-species-notes": " Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50 - 1/100", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "" }, "Recombinant full length protein - Human": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "" } } }

style

left-column

Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Stable for 12 months at -20°C

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ARF1 and ARF3 also known as ADP-ribosylation factors 1 and 3 are small GTP-binding proteins belonging to the ARF family. ARF1 has a molecular mass of approximately 21 kDa. These proteins are expressed in most eukaryotic cells where they play roles in vesicular trafficking processes. ARF1 is mainly located in the Golgi apparatus while ARF3 appears in the plasma membrane and cytoplasm. They function as molecular switches by cycling between active GTP-bound and inactive GDP-bound states which regulates the formation and budding of vesicles.

Biological function summary

ARF1 and ARF3 are significant in the intracellular trafficking of proteins and lipids. The proteins are part of the coatomer protein complex (COPI) important for retrograde transport from the Golgi to the endoplasmic reticulum. By recruiting coat proteins to the Golgi membranes ARF1 and ARF3 facilitate the sorting and packaging of cargo molecules into transport vesicles. This process sustains the maintenance of organelle identity and the dynamics of membrane remodeling.

Pathways

ARF1 and ARF3 are involved in key cellular pathways particularly those related to vesicular trafficking and membrane dynamics. They participate in the COPI and clathrin-mediated pathways. Through these pathways ARF1 interacts with proteins like PDZD8 while ARF3 works with proteins involved in primary cilia formation. These interactions underline their integrative role in maintaining proper cellular organization and communication.

ARF1 and ARF3 have links to cancer and neurodegenerative diseases. Dysregulation of ARF1 is associated with various cancer types due to its role in cell proliferation and migration. ARF3 has connections to neurodegenerative disorders where altered vesicular trafficking can lead to pathological conditions. ARF1's interaction with the protein NFDM-442 highlights its importance in disease contexts whereby changes in these interactions might contribute to disease progression and severity.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Small GTPase involved in protein trafficking between different compartments (PubMed : 8253837). Modulates vesicle budding and uncoating within the Golgi complex (PubMed : 8253837). In its GTP-bound form, triggers the recruitment of coatomer proteins to the Golgi membrane (PubMed : 8253837). The hydrolysis of ARF1-bound GTP, which is mediated by ARFGAPs proteins, is required for dissociation of coat proteins from Golgi membranes and vesicles (PubMed : 8253837). The GTP-bound form interacts with PICK1 to limit PICK1-mediated inhibition of Arp2/3 complex activity; the function is linked to AMPA receptor (AMPAR) trafficking, regulation of synaptic plasticity of excitatory synapses and spine shrinkage during long-term depression (LTD) (By similarity). Plays a key role in the regulation of intestinal stem cells and gut microbiota, and is essential for maintaining intestinal homeostasis (By similarity). Also plays a critical role in mast cell expansion but not in mast cell maturation by facilitating optimal mTORC1 activation (By similarity).. (Microbial infection) Functions as an allosteric activator of the cholera toxin catalytic subunit, an ADP-ribosyltransferase.

See full target information ARF1

Additional targets

ADP-ribosylation factor 3

style

left-column

Publications (7)

Recent publications for all applications. Explore the full list and refine your search

Acta biochimica Polonica 68:223-229 PubMed33847108

2021

ARF3 inhibits proliferation and promotes apoptosis in gastric cancer by regulating AKT and ERK pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Jiayun Liu,Shenlin Liu,Maolin Wu,Jing Deng,Xuequan Yao,Fukun Liu,Xiaoyu Wu,Guannan Wu

The Journal of pathology 253:106-118 PubMed33009820

2020

Histone demethylase PHF8 drives neuroendocrine prostate cancer progression by epigenetically upregulating FOXA2.

Applications

Unspecified application

Species

Unspecified reactive species

Qiuli Liu,Jian Pang,Lin-Ang Wang,Zhuowei Huang,Jing Xu,Xingxia Yang,Qiubo Xie,Yiqiang Huang,Tang Tang,Dali Tong,Gaolei Liu,Luofu Wang,Dianzheng Zhang,Qiang Ma,Hualiang Xiao,Weihua Lan,Jun Qin,Jun Jiang

International immunopharmacology 75:105836 PubMed31450153

2019

TRPM2 channel regulates cytokines production in astrocytes and aggravates brain disorder during lipopolysaccharide-induced endotoxin sepsis.

Applications

Unspecified application

Species

Unspecified reactive species

Tao Zhu,Yisha Zhao,Hui Hu,Qianqian Zheng,Xiaoying Luo,Yinjie Ling,Yingchao Ying,Zheng Shen,Peifang Jiang,Qiang Shu

The Journal of cell biology 216:181-197 PubMed28007915

2016

Podosome assembly is controlled by the GTPase ARF1 and its nucleotide exchange factor ARNO.

Applications

Species

Unspecified reactive species

Nisha Bte Mohd Rafiq,Zi Zhao Lieu,Tingting Jiang,Cheng-Han Yu,Paul Matsudaira,Gareth E Jones,Alexander D Bershadsky

The Journal of biological chemistry 291:462-77 PubMed26507660

2015

Protein Kinase D2 Assembles a Multiprotein Complex at the Trans-Golgi Network to Regulate Matrix Metalloproteinase Secretion.

Applications

Unspecified application

Species

Unspecified reactive species

Tim Eiseler,Christoph Wille,Conny Koehler,Anett Illing,Thomas Seufferlein

PloS one 9:e96647 PubMed24792215

2014

CLN3 deficient cells display defects in the ARF1-Cdc42 pathway and actin-dependent events.

Applications

Species

Mouse

Mark L Schultz,Luis Tecedor,Colleen S Stein,Mark A Stamnes,Beverly L Davidson

PloS one 7:e44572 PubMed22962618

2012

Secretion of soluble vascular endothelial growth factor receptor 1 (sVEGFR1/sFlt1) requires Arf1, Arf6, and Rab11 GTPases.

Applications

Unspecified application

Species

Unspecified reactive species

Jae-Joon Jung,Ajit Tiwari,Shivangi M Inamdar,Christie P Thomas,Apollina Goel,Amit Choudhury

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-ARF1 + ARF3 antibody [EPR443]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARF1 + ARF3 antibody [EPR443] (AB108347)

Immunocytochemistry/ Immunofluorescence - Anti-ARF1 + ARF3 antibody [EPR443] (AB108347)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARF1 + ARF3 antibody [EPR443] (AB108347)

Western blot - Anti-ARF1 + ARF3 antibody [EPR443] (AB108347)

Western blot - Anti-ARF1 + ARF3 antibody [EPR443] (AB108347)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

Alternative Products

Primary Antibodies

Primary Antibodies

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Additional targets

Publications (7)

Product promise