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AB129003

Anti-ARF1 + ARF3 antibody [EPR8304(B)]

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(4 Publications)

Rabbit Recombinant Monoclonal ARF1 antibody. Suitable for IHC-P, WB and reacts with Human, Recombinant full length protein - Human samples. Cited in 4 publications.

View Alternative Names

ADP-ribosylation factor 1, ARF1, ADP-ribosylation factor 3, ARF3

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARF1 + ARF3 antibody [EPR8304(B)] (AB129003)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARF1 + ARF3 antibody [EPR8304(B)] (AB129003)

ab129003, at 1/50 dilution, staining ARF3 in paraffin-embedded Human stomach tissue by Immunohistochemistry.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Western blot - Anti-ARF1 + ARF3 antibody [EPR8304(B)] (AB129003)
  • WB

Lab

Western blot - Anti-ARF1 + ARF3 antibody [EPR8304(B)] (AB129003)

Blocking and diluting buffer : 5% NFDM/TBST

Human ARF-1 recombinant protein, (Cat# : ab123538 )
Human ARF-3 recombinant protein, (Cat# : ab113581 )

All lanes:

Western blot - Anti-ARF1 + ARF3 antibody [EPR8304(B)] (ab129003) at 1/20000 dilution

Lane 1:

Full length human ARF-1 recombinant protein at 0.015 µg

Lane 2:

Full length human ARF-3 recombinant protein at 0.015 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 20 kDa

Observed band size: 23 kDa

false

Exposure time: 3min

Western blot - Anti-ARF1 + ARF3 antibody [EPR8304(B)] (AB129003)
  • WB

Unknown

Western blot - Anti-ARF1 + ARF3 antibody [EPR8304(B)] (AB129003)

All lanes:

Western blot - Anti-ARF1 + ARF3 antibody [EPR8304(B)] (ab129003) at 1/10000 dilution

Lane 1:

HeLa cell lysate at 10 µg

Lane 2:

HepG2 cell lysate at 10 µg

Lane 3:

Human fetal brain lysate at 10 µg

Lane 4:

Raji cell lysate at 10 µg

Secondary

All lanes:

Goat anti-Rabbit HRP at 1/2000 dilution

Predicted band size: 20 kDa

Observed band size: 20 kDa

false

  • Carrier free

    Anti-ARF1+ARF3 antibody [EPR8304(B)] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR8304(B)

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Stable for 12 months at -20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ARF1 and ARF3 also known as ADP-ribosylation factors 1 and 3 are small GTP-binding proteins belonging to the ARF family. ARF1 has a molecular mass of approximately 21 kDa. These proteins are expressed in most eukaryotic cells where they play roles in vesicular trafficking processes. ARF1 is mainly located in the Golgi apparatus while ARF3 appears in the plasma membrane and cytoplasm. They function as molecular switches by cycling between active GTP-bound and inactive GDP-bound states which regulates the formation and budding of vesicles.
Biological function summary

ARF1 and ARF3 are significant in the intracellular trafficking of proteins and lipids. The proteins are part of the coatomer protein complex (COPI) important for retrograde transport from the Golgi to the endoplasmic reticulum. By recruiting coat proteins to the Golgi membranes ARF1 and ARF3 facilitate the sorting and packaging of cargo molecules into transport vesicles. This process sustains the maintenance of organelle identity and the dynamics of membrane remodeling.

Pathways

ARF1 and ARF3 are involved in key cellular pathways particularly those related to vesicular trafficking and membrane dynamics. They participate in the COPI and clathrin-mediated pathways. Through these pathways ARF1 interacts with proteins like PDZD8 while ARF3 works with proteins involved in primary cilia formation. These interactions underline their integrative role in maintaining proper cellular organization and communication.

ARF1 and ARF3 have links to cancer and neurodegenerative diseases. Dysregulation of ARF1 is associated with various cancer types due to its role in cell proliferation and migration. ARF3 has connections to neurodegenerative disorders where altered vesicular trafficking can lead to pathological conditions. ARF1's interaction with the protein NFDM-442 highlights its importance in disease contexts whereby changes in these interactions might contribute to disease progression and severity.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Small GTPase involved in protein trafficking between different compartments (PubMed : 8253837). Modulates vesicle budding and uncoating within the Golgi complex (PubMed : 8253837). In its GTP-bound form, triggers the recruitment of coatomer proteins to the Golgi membrane (PubMed : 8253837). The hydrolysis of ARF1-bound GTP, which is mediated by ARFGAPs proteins, is required for dissociation of coat proteins from Golgi membranes and vesicles (PubMed : 8253837). The GTP-bound form interacts with PICK1 to limit PICK1-mediated inhibition of Arp2/3 complex activity; the function is linked to AMPA receptor (AMPAR) trafficking, regulation of synaptic plasticity of excitatory synapses and spine shrinkage during long-term depression (LTD) (By similarity). Plays a key role in the regulation of intestinal stem cells and gut microbiota, and is essential for maintaining intestinal homeostasis (By similarity). Also plays a critical role in mast cell expansion but not in mast cell maturation by facilitating optimal mTORC1 activation (By similarity).. (Microbial infection) Functions as an allosteric activator of the cholera toxin catalytic subunit, an ADP-ribosyltransferase.
See full target information ARF1

Additional targets

ADP-ribosylation factor 3
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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Cell death & disease 16:675 PubMed41053099

2025

Adipose-tumor crosstalk in colorectal cancer: Identifying (Epi)genetic biomarkers for tumor progression and cachexia.

Applications

Unspecified application

Species

Unspecified reactive species

Ada Pesapane,Lucia Capasso,Maria Rosaria Del Sorbo,Lucia Scisciola,Teresa Troiani,Donato Mele,Martina Franzese,Armando Puocci,Giovanni Tortorella,Surina Surina,Giacomo Fuschillo,Francesco Caraglia,Vincenzo De Falco,Lucio Selvaggi,Rosaria Anna Fontanella,Fortunato Ciardiello,Francesco Selvaggi,Lucia Altucci,Giuseppe Paolisso,Michelangela Barbieri,Angela Nebbioso

Journal of lipid research 64:100362 PubMed36958722

2023

Cholesterol synthesis enzyme SC4MOL is fine-tuned by sterols and targeted for degradation by the E3 ligase MARCHF6.

Applications

Unspecified application

Species

Unspecified reactive species

Lydia Qian,Nicola A Scott,Isabelle M Capell-Hattam,Eliza A Draper,Nicole M Fenton,Winnie Luu,Laura J Sharpe,Andrew J Brown

Breast cancer research : BCR 24:40 PubMed35681213

2022

Multiple roles for Bcl-3 in mammary gland branching, stromal collagen invasion, involution and tumor pathology.

Applications

Unspecified application

Species

Unspecified reactive species

David Carr,Aiman Zein,Josée Coulombe,Tianqi Jiang,Miguel A Cabrita,Gwendoline Ward,Manijeh Daneshmand,Andrea Sau,M A Christine Pratt

Frontiers in immunology 12:714799 PubMed34721381

2021

The HHV-6A Proteins U20 and U21 Target NKG2D Ligands to Escape Immune Recognition.

Applications

Unspecified application

Species

Unspecified reactive species

Abigael Eva Chaouat,Barbara Seliger,Ofer Mandelboim,Dominik Schmiedel
View all publications
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Product promise

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