AB247266

Anti-ARF1+ARF3 antibody [EP442Y] - BSA and Azide free

RabMAb
Recombinant
What is this?

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(1 Publication)

Rabbit Recombinant Monoclonal ARF1 antibody. Carrier free. Suitable for IHC-P, ICC/IF, WB, Flow Cyt (Intra) and reacts with Human, Recombinant full length protein - Human samples. Cited in 1 publication.

View Alternative Names

ADP-ribosylation factor 1, ARF1, ADP-ribosylation factor 3, ARF3

3 Images

Immunocytochemistry/ Immunofluorescence - Anti-ARF1+ARF3 antibody [EP442Y] - BSA and Azide free (AB247266)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-ARF1+ARF3 antibody [EP442Y] - BSA and Azide free (AB247266)

This data was developed using ab32524, the same antibody clone in a different buffer formulation.

Immunofluorescence staining of MCF7 cells with purified ab32524 working dilution of 1/500, counter-stained with DAPI. The secondary antibody was an Alexa Fluor^® 488 conjugated goat anti-rabbit (ab150077), used at a dilution of 1/1000. The cells were fixed in 100% methanol. The negative control is shown in bottom right hand panel - for the negative control, PBS was used instead of the primary antibody.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARF1+ARF3 antibody [EP442Y] - BSA and Azide free (AB247266)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARF1+ARF3 antibody [EP442Y] - BSA and Azide free (AB247266)

This data was developed using ab32524, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded human breast carcinoma using ab32524 at 1/250 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Lab

Western blot - Anti-ARF1+ARF3 antibody [EP442Y] - BSA and Azide free (AB247266)

This data was developed using ab32524, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

Human ARF-1 recombinant protein (ab123538 ), Human ARF-3 recombinant protein (ab113581).

All lanes:

Western blot - Anti-ARF1 + ARF3 antibody [EP442Y] (<a href='/en-us/products/primary-antibodies/arf1-arf3-antibody-ep442y-ab32524'>ab32524</a>) at 1/20000 dilution

Lane 1:

Full length human ARF-1 recombinant protein at 0.015 µg

Lane 2:

Full length human ARF-3 recombinant protein at 0.015 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 21 kDa

Observed band size: 23 kDa

false

Exposure time: 3min

Unconjugated

Anti-ARF1 + ARF3 antibody [EP442Y]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EP442Y

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

ICC/IF, IHC-P, Flow Cyt (Intra), WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

The immunogen used for this product shares 100% homology with ARF3, ARF4 and ARF5. Cross-reactivity with the latter two protein have not been confirmed experimentally.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Recombinant full length protein - Human": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "" } } }

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Product details

ab247266 is the carrier-free version of ab32524.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ARF1 and ARF3 are small GTP-binding proteins part of the ADP-ribosylation factor (ARF) family involved in vesicle formation and trafficking. Alternate names for these proteins include ADP-ribosylation factor 1 and 3. ARF1 has a molecular mass of about 21 kDa and ARF3 is similar in size. These proteins localize primarily to the Golgi apparatus and cytoplasmic regions including the endoplasmic reticulum and plasma membrane. The expression of ARF1 and ARF3 is widespread occurring in various cell types across different tissues indicating roles in fundamental cellular processes.

Biological function summary

ARF1 and ARF3 regulate the assembly of coat protein complexes on membrane surfaces which is essential for vesicle formation. They participate in the transport of proteins and lipids between cellular organelles particularly in the secretory and endocytic pathways. ARF1 specifically functions in activating phospholipase D and is part of large protein complexes that involve coatomer proteins and clathrin. While they share overlapping functions ARF1 and ARF3 exhibit some distinct roles in vesicle trafficking indicating the specificity in biological functions.

Pathways

ARF1 and ARF3 play important roles in the regulation of the Golgi apparatus and endocytic pathways. They are important in coordinating vesicular transport by interacting with proteins such as COPI and clathrin facilitating cargo sorting and trafficking. ARF1 is also linked to the phosphoinositide metabolic pathway influencing membrane dynamics and trafficking processes. Whereas ARF3 is involved in pathways that modulate cytoskeletal functions indicating its diverse effects within related cellular signaling and trafficking pathways.

Defects in ARF1 and ARF3 functions can contribute to conditions such as cancer and neurodegenerative diseases. Aberrant ARF1 expression has connections to cancer progression due to its influence on cell migration and invasion possibly linked with proteins like clathrin that modulate vesicular trafficking. Altered ARF3 activity associates with neurodegenerative disorders involving connections with proteins in signaling pathways related to ARF1 impacting neuronal transport and synaptic functions. These proteins' involvement in cellular trafficking highlights their roles in maintaining cellular homeostasis and their links to disease states.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Small GTPase involved in protein trafficking between different compartments (PubMed : 8253837). Modulates vesicle budding and uncoating within the Golgi complex (PubMed : 8253837). In its GTP-bound form, triggers the recruitment of coatomer proteins to the Golgi membrane (PubMed : 8253837). The hydrolysis of ARF1-bound GTP, which is mediated by ARFGAPs proteins, is required for dissociation of coat proteins from Golgi membranes and vesicles (PubMed : 8253837). The GTP-bound form interacts with PICK1 to limit PICK1-mediated inhibition of Arp2/3 complex activity; the function is linked to AMPA receptor (AMPAR) trafficking, regulation of synaptic plasticity of excitatory synapses and spine shrinkage during long-term depression (LTD) (By similarity). Plays a key role in the regulation of intestinal stem cells and gut microbiota, and is essential for maintaining intestinal homeostasis (By similarity). Also plays a critical role in mast cell expansion but not in mast cell maturation by facilitating optimal mTORC1 activation (By similarity).. (Microbial infection) Functions as an allosteric activator of the cholera toxin catalytic subunit, an ADP-ribosyltransferase.

See full target information ARF1

Additional targets

ADP-ribosylation factor 3

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

OncoTargets and therapy 18:1093-1105 PubMed41019790

2025

Ebp1 p48 Promotes Oncogenic Properties in Non-Small Cell Lung Cancer Through PI3K/Akt Signaling Pathways.

Applications

Unspecified application

Species

Unspecified reactive species

Lina Ma,Shuyuan Wang,Jia Zhang,Mengyuan Xin,Dongyuan Xu,Lan Liu,Xiangdan Li

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-ARF1+ARF3 antibody [EP442Y] - BSA and Azide free

Immunocytochemistry/ Immunofluorescence - Anti-ARF1+ARF3 antibody [EP442Y] - BSA and Azide free (AB247266)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARF1+ARF3 antibody [EP442Y] - BSA and Azide free (AB247266)

Western blot - Anti-ARF1+ARF3 antibody [EP442Y] - BSA and Azide free (AB247266)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Additional targets

Publications (1)

Product promise