Rabbit Recombinant Multiclonal ARF1 antibody. Suitable for WB and reacts with Transfected cell lysate - Human, Human samples.
IgG
Rabbit
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Liquid
Multiclonal
WB | ICC/IF | IHC-P | |
---|---|---|---|
Human | Tested | Not recommended | Not recommended |
Transfected cell lysate - Human | Tested | Not recommended | Not recommended |
Species | Dilution info | Notes |
---|---|---|
Species Transfected cell lysate - Human | Dilution info 1/5000 | Notes - |
Species Human | Dilution info 1/1000 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human, Transfected cell lysate - Human | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human, Transfected cell lysate - Human | Dilution info - | Notes - |
Small GTPase involved in protein trafficking between different compartments (PubMed:8253837). Modulates vesicle budding and uncoating within the Golgi complex (PubMed:8253837). In its GTP-bound form, triggers the recruitment of coatomer proteins to the Golgi membrane (PubMed:8253837). The hydrolysis of ARF1-bound GTP, which is mediated by ARFGAPs proteins, is required for dissociation of coat proteins from Golgi membranes and vesicles (PubMed:8253837). The GTP-bound form interacts with PICK1 to limit PICK1-mediated inhibition of Arp2/3 complex activity; the function is linked to AMPA receptor (AMPAR) trafficking, regulation of synaptic plasticity of excitatory synapses and spine shrinkage during long-term depression (LTD) (By similarity). Plays a key role in the regulation of intestinal stem cells and gut microbiota, and is essential for maintaining intestinal homeostasis (By similarity). Plays also a critical role in mast cell expansion but not in mast cell maturation by facilitating optimal mTORC1 activation (By similarity).(Microbial infection) Functions as an allosteric activator of the cholera toxin catalytic subunit, an ADP-ribosyltransferase.
ADP-ribosylation factor 3, ADP-ribosylation factor 4, ADP-ribosylation factor 6
ADP-ribosylation factor 1, ARF1
Rabbit Recombinant Multiclonal ARF1 antibody. Suitable for WB and reacts with Transfected cell lysate - Human, Human samples.
IgG
Rabbit
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Liquid
Multiclonal
RM1272
Affinity purification Protein A
Blue Ice
1-2 weeks
+4°C
-20°C
Upon delivery aliquot
Avoid freeze / thaw cycle
This product is a recombinant multiclonal antibody, which offers several advantages including:
For more information, read more on recombinant antibodies.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We have tested this species and application combination and it works. It is covered by our product promise.
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
ab322396 reacts with human ARF1/3/4/6.
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.
In Western blot, Anti-6X His tag® antibody [EPR20547] - ChIP Grade (Anti-6X His tag® antibody [EPR20547] - ChIP Grade ab213204) staining at 1/5000 dilution.
All lanes: Western blot - Anti-ARF1+ARF3+ARF4+ARF6 antibody [RM1272] (ab322396) at 1/5000 dilution
Lane 1: NIH/3T3 (mouse embryonic fibroblast) transfected with an empty vector containing a His tag, whole cell lysate at 20 µg
Lane 2: NIH/3T3 transfected with a human ARF4 expression vector containing a His tag, whole cell lysate at 20 µg
Lane 3: NIH/3T3 transfected with a human ARF6 expression vector containing a His tag, whole cell lysate at 20 µg
Lane 4: NIH/3T3 transfected with a human ARF3 expression vector containing a His tag, whole cell lysate at 20 µg
Lane 5: NIH/3T3 transfected with a human ARF1 expression vector containing a His tag, whole cell lysate at 20 µg
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Observed band size: 20 kDa, 36 kDa
Exposure time: 37s
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
Low expression: heart (PMID: 8702973).
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.
All lanes: Western blot - Anti-ARF1+ARF3+ARF4+ARF6 antibody [RM1272] (ab322396) at 1/1000 dilution
Lane 1: Human kidney tissue lysate at 20 µg
Lane 2: Human heart tissue lysate at 20 µg
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Observed band size: 18 kDa, 36 kDa
Exposure time: 180s
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
Low expression: skeletal muscle (PMID: 8702973).
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.
All lanes: Western blot - Anti-ARF1+ARF3+ARF4+ARF6 antibody [RM1272] (ab322396) at 1/1000 dilution
Lane 1: MCF7 (human breast adenocarcinoma epithelial cell) whole cell lysate at 20 µg
Lane 2: SiHa (human cervical epithelial cell) whole cell lysate at 20 µg
Lane 3: T-47D (human ductal breast epithelial tumor epithelial cell) whole cell lysate at 20 µg
Lane 4: HT-29 (human colorectal adenocarcinoma epithelial cell) whole cell lysate at 20 µg
Lane 5: HepG2 (human hepatocellular carcinoma epithelial cell) whole cell lysate at 20 µg
Lane 6: K-562 (human chronic myelogenous leukemia lymphoblast) whole cell lysate at 20 µg
Lane 7: HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate at 20 µg
Lane 8: Human skeletal muscle tissue lysate at 20 µg
Lane 9: Human liver tissue lysate at 20 µg
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Observed band size: 18 kDa, 36 kDa
Exposure time: 59s
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