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Rabbit Recombinant Multiclonal ARF1 antibody. Suitable for WB and reacts with Transfected cell lysate - Human, Human samples.

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Images

Western blot - Anti-ARF1+ARF3+ARF4+ARF6 antibody [RM1272] (AB322396), expandable thumbnail
  • Western blot - Anti-ARF1+ARF3+ARF4+ARF6 antibody [RM1272] (AB322396), expandable thumbnail
  • Western blot - Anti-ARF1+ARF3+ARF4+ARF6 antibody [RM1272] (AB322396), expandable thumbnail

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form

Liquid

Clonality

Multiclonal

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBICC/IFIHC-P
Human
Tested
Not recommended
Not recommended
Transfected cell lysate - Human
Tested
Not recommended
Not recommended

Tested
Tested

Species

Transfected cell lysate - Human

Dilution info

1/5000

Notes

-

Species

Human

Dilution info

1/1000

Notes

-

Not recommended
Not recommended

Species

Human, Transfected cell lysate - Human

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Human, Transfected cell lysate - Human

Dilution info

-

Notes

-

Target data

Function

Small GTPase involved in protein trafficking between different compartments (PubMed:8253837). Modulates vesicle budding and uncoating within the Golgi complex (PubMed:8253837). In its GTP-bound form, triggers the recruitment of coatomer proteins to the Golgi membrane (PubMed:8253837). The hydrolysis of ARF1-bound GTP, which is mediated by ARFGAPs proteins, is required for dissociation of coat proteins from Golgi membranes and vesicles (PubMed:8253837). The GTP-bound form interacts with PICK1 to limit PICK1-mediated inhibition of Arp2/3 complex activity; the function is linked to AMPA receptor (AMPAR) trafficking, regulation of synaptic plasticity of excitatory synapses and spine shrinkage during long-term depression (LTD) (By similarity). Plays a key role in the regulation of intestinal stem cells and gut microbiota, and is essential for maintaining intestinal homeostasis (By similarity). Plays also a critical role in mast cell expansion but not in mast cell maturation by facilitating optimal mTORC1 activation (By similarity).(Microbial infection) Functions as an allosteric activator of the cholera toxin catalytic subunit, an ADP-ribosyltransferase.

Targets

ADP-ribosylation factor 3, ADP-ribosylation factor 4, ADP-ribosylation factor 6

Alternative names

Rabbit Recombinant Multiclonal ARF1 antibody. Suitable for WB and reacts with Transfected cell lysate - Human, Human samples.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Multiclonal

Clone number

RM1272

Purification technique

Affinity purification Protein A

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

This product is a recombinant multiclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

3 product images

  • Western blot - Anti-ARF1+ARF3+ARF4+ARF6 antibody [RM1272] (ab322396), expandable thumbnail

    Western blot - Anti-ARF1+ARF3+ARF4+ARF6 antibody [RM1272] (ab322396)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    ab322396 reacts with human ARF1/3/4/6.

    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

    In Western blot, Anti-6X His tag® antibody [EPR20547] - ChIP Grade (Anti-6X His tag® antibody [EPR20547] - ChIP Grade ab213204) staining at 1/5000 dilution.

    All lanes: Western blot - Anti-ARF1+ARF3+ARF4+ARF6 antibody [RM1272] (ab322396) at 1/5000 dilution

    Lane 1: NIH/3T3 (mouse embryonic fibroblast) transfected with an empty vector containing a His tag, whole cell lysate at 20 µg

    Lane 2: NIH/3T3 transfected with a human ARF4 expression vector containing a His tag, whole cell lysate at 20 µg

    Lane 3: NIH/3T3 transfected with a human ARF6 expression vector containing a His tag, whole cell lysate at 20 µg

    Lane 4: NIH/3T3 transfected with a human ARF3 expression vector containing a His tag, whole cell lysate at 20 µg

    Lane 5: NIH/3T3 transfected with a human ARF1 expression vector containing a His tag, whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Observed band size: 20 kDa, 36 kDa

    Exposure time: 37s

  • Western blot - Anti-ARF1+ARF3+ARF4+ARF6 antibody [RM1272] (ab322396), expandable thumbnail

    Western blot - Anti-ARF1+ARF3+ARF4+ARF6 antibody [RM1272] (ab322396)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Low expression: heart (PMID: 8702973).

    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

    All lanes: Western blot - Anti-ARF1+ARF3+ARF4+ARF6 antibody [RM1272] (ab322396) at 1/1000 dilution

    Lane 1: Human kidney tissue lysate at 20 µg

    Lane 2: Human heart tissue lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Observed band size: 18 kDa, 36 kDa

    Exposure time: 180s

  • Western blot - Anti-ARF1+ARF3+ARF4+ARF6 antibody [RM1272] (ab322396), expandable thumbnail

    Western blot - Anti-ARF1+ARF3+ARF4+ARF6 antibody [RM1272] (ab322396)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Low expression: skeletal muscle (PMID: 8702973).

    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

    All lanes: Western blot - Anti-ARF1+ARF3+ARF4+ARF6 antibody [RM1272] (ab322396) at 1/1000 dilution

    Lane 1: MCF7 (human breast adenocarcinoma epithelial cell) whole cell lysate at 20 µg

    Lane 2: SiHa (human cervical epithelial cell) whole cell lysate at 20 µg

    Lane 3: T-47D (human ductal breast epithelial tumor epithelial cell) whole cell lysate at 20 µg

    Lane 4: HT-29 (human colorectal adenocarcinoma epithelial cell) whole cell lysate at 20 µg

    Lane 5: HepG2 (human hepatocellular carcinoma epithelial cell) whole cell lysate at 20 µg

    Lane 6: K-562 (human chronic myelogenous leukemia lymphoblast) whole cell lysate at 20 µg

    Lane 7: HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate at 20 µg

    Lane 8: Human skeletal muscle tissue lysate at 20 µg

    Lane 9: Human liver tissue lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Observed band size: 18 kDa, 36 kDa

    Exposure time: 59s

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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