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AB154383

Anti-ARF3 antibody

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(2 Publications )

Rabbit Polyclonal ARF3 antibody. Suitable for WB and reacts with Mouse samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human ADP-ribosylation factor 3 aa 1-150.
1 Images
Western blot - Anti-ARF3 antibody (AB154383)
  • WB

Unknown

Western blot - Anti-ARF3 antibody (AB154383)

12% SDS PAGE

All lanes:

Western blot - Anti-ARF3 antibody (ab154383) at 1/1000 dilution

All lanes:

Mouse brain whole cell lysate at 50 µg

Predicted band size: 20 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse

Applications

WB

applications

Immunogen

Recombinant Fragment Protein within Human ADP-ribosylation factor 3 aa 1-150. The exact immunogen used to generate this antibody is proprietary information.

P61204

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Proteins & Peptides

AB113581

Recombinant Human ARF3 protein (His tag N-Terminus)

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Primary Antibodies

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Primary Antibodies

AB214274

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Pig": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Xenopus laevis": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Xenopus tropicalis": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 20% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ADP-ribosylation factor 3 commonly known as ARF3 is a small GTP-binding protein with a mass of approximately 20 kDa. It belongs to the ARF family and is expressed in many tissues especially within cellular compartments that are highly dynamic including the Golgi apparatus and the cytoplasm. ARF3 functions by binding and hydrolyzing GTP which is an important function in regulating vesicular trafficking and membrane lipid metabolism.
Biological function summary

The functions performed by ARF3 revolve around the regulation of vesicle formation and trafficking within cells. It is critical in the formation of COPI-coated vesicles and is involved in the retrieval pathway of membrane proteins between the Golgi and endoplasmic reticulum. ARF3 interacts closely with proteins like coatomer and ARF-GEFs (guanine nucleotide exchange factors) to facilitate these processes. As part of its biological activity ARF3 affects the recruitment of various effector proteins necessary for the vesicular transport.

Pathways

ARF3 plays significant roles in the regulation of intracellular transport pathways and lipid signaling pathways. It interacts with proteins involved in the phosphatidylinositol signaling pathway influencing membrane dynamics and cell signaling. ARF3's function aligns it closely with proteins such as phosphatidylinositol 4-kinases which are key in modifying phosphoinositides that are essential for membrane trafficking and signal transduction.

Connections between ARF3 and pathological conditions like cancer and neurodegenerative diseases have been observed. Abnormal expression or mutation of ARF3 may lead to disruptions in membrane trafficking contributing to disease progression. ARF3 has shown links with proteins such as RAB proteins in the context of cancer as they collaboratively affect cellular processes relevant to tumor progression such as cellular migration and invasion.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

GTP-binding protein that functions as an allosteric activator of the cholera toxin catalytic subunit, an ADP-ribosyltransferase. Involved in protein trafficking; may modulate vesicle budding and uncoating within the Golgi apparatus.
See full target information ADP-ribosylation factor 3

Alternative Names

ADP-ribosylation factor 3, ARF3
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Publications (2)

Recent publications for all applications. Explore the full listand refine your search

eLife 11: PubMed35259089

2022

Quantitative transportomics identifies Kif5a as a major regulator of neurodegeneration.

Applications

Unspecified application

Species

Unspecified reactive species

Sahil H Shah,Lucio M Schiapparelli,Yuanhui Ma,Satoshi Yokota,Melissa Atkins,Xin Xia,Evan G Cameron,Thanh Huang,Sarah Saturday,Catalina B Sun,Cara Knasel,Seth Blackshaw,John R Yates,Hollis T Cline,Jeffrey L Goldberg

Ecology and evolution 9:6968-6985 PubMed31467669

2019

Genomewide identification and analysis of heat-shock proteins 70/110 to reveal their potential functions in Chinese soft-shelled turtle .

Applications

Unspecified application

Species

Unspecified reactive species

Tengfei Liu,Yawen Han,Ye Liu,Huiying Zhao
View all publications
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Product promise

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