AB226389

Anti-ARF6 antibody

Name: Anti-ARF6 antibody (ab226389) | Abcam
Brand: Abcam Limited
SKU: AB226389

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(6 Publications)

Rabbit Polyclonal ARF6 antibody. Suitable for IP, WB and reacts with Human, Mouse samples. Cited in 6 publications. Immunogen corresponding to Synthetic Peptide within Human ADP-ribosylation factor 6 aa 100 to C-terminus.

View Alternative Names

ADP-ribosylation factor 6, ARF6

2 Images

Supplier Data

Immunoprecipitation - Anti-ARF6 antibody (AB226389)

ARF6 was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (prepared using NETN buffer; 0.5 or 1.0 mg for IP, 20% of IP loaded) with ab226389 at 6 μg/mg lysate. Western blot was performed from the immunoprecipitate using ab226389 at 1 μg/ml.

Lane 1 : ab226389 IP in HEK-293T whole cell lysate.

Lane 2 : Control IgG IP in HEK-293T whole cell lysate.

Detection : Chemiluminescence with exposure time of 10 seconds.

All lanes:

Immunoprecipitation - Anti-ARF6 antibody (ab226389)

Predicted band size: 20 kDa

true

Exposure time: 10s

Supplier Data

Western blot - Anti-ARF6 antibody (AB226389)

All lanes:

Western blot - Anti-ARF6 antibody (ab226389) at 0.1 µg/mL

Lane 1:

HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate at 50 µg

Lane 2:

HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate at 50 µg

Lane 3:

Jurkat (human T cell leukemia cell line from peripheral blood) cell lysate at 50 µg

Lane 4:

TCMK-1 (mouse kidney epithelial cell line) cell lysate at 50 µg

Lane 5:

NIH/3T3 (mouse embryo fibroblast cell line) cell lysate at 50 µg

Predicted band size: 20 kDa

true

Exposure time: 30s

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

WB, IP

applications

Immunogen

Synthetic Peptide within Human ADP-ribosylation factor 6 aa 100 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P62330

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-10 µg/mg of lysate", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "" }, "Rat": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Chimpanzee": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Dog": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Gorilla": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Guinea pig": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Horse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rabbit": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rhesus monkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Sheep": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: Tris citrate/phosphate

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The ARF6 protein also known as ADP-ribosylation factor 6 is a small GTPase with a molecular weight of approximately 21 kDa. The protein belongs to the ARF family which plays roles in regulating vesicular trafficking and cytoskeletal dynamics. ARF6 is ubiquitously expressed across many tissues most abundantly in the brain muscle and epithelial cells. Its activity depends on cycling between an inactive GDP-bound form and an active GTP-bound form which is important for its function in cellular processes.

Biological function summary

The activity of ARF6 influences membrane trafficking and actin cytoskeleton remodeling. The protein helps mediate endocytosis and exocytosis as well as modulating cell adhesion and migration. ARF6 interacts with various effector proteins to form a complex that is involved in the coordination of these processes. This complex activity underlines ARF6's roles in cell signaling and intracellular trafficking mechanisms.

Pathways

ARF6 plays key roles in the Wnt signaling pathway and the E-cadherin recycling pathway. In the Wnt pathway ARF6 regulates the internalization of receptors and affects downstream signaling. In E-cadherin recycling ARF6 orchestrates trafficking events that ensure proper cell-cell adhesion. These functions often involve interactions with related proteins like ARF1 and Rac1 which further modulate ARF6's involvement in these signaling events influencing cellular behavior.

ARF6 has a connection to certain cancers and neurological conditions. Its dysregulation can lead to increased metastasis in cancers partly due to altered cell motility and invasion linked to changes in cell adhesion. Additionally the misregulation of ARF6 is implicated in Alzheimer's disease where it may disrupt neuronal trafficking and contribute to synaptic dysfunction. These disease associations also involve related proteins such as EFA6 and ARHGAP10 which can modulate ARF6 activity and its effects on pathological processes.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

GTP-binding protein involved in protein trafficking that regulates endocytic recycling and cytoskeleton remodeling (PubMed : 11266366, PubMed : 16737952, PubMed : 18400762, PubMed : 21170023, PubMed : 32103017, PubMed : 7589240). GTP-bound form plays an important role in the transport of multiple palmitoylated proteins form the Golgi to the plasma membrane (PubMed : 37461827). Required for normal completion of mitotic cytokinesis (By similarity). Plays a role in the reorganization of the actin cytoskeleton and the formation of stress fibers (By similarity). Involved in the regulation of dendritic spine development, contributing to the regulation of dendritic branching and filopodia extension (PubMed : 14978216). Potentiates the neurite outgrowth in primary neurons by interacting with the molecular adapter APBB1 (PubMed : 36250347). Plays an important role in membrane trafficking, during junctional remodeling and epithelial polarization (PubMed : 36017701). Regulates surface levels of adherens junction proteins such as CDH1 (By similarity). Required for NTRK1 sorting to the recycling pathway from early endosomes (By similarity).. (Microbial infection) Functions as an allosteric activator of the cholera toxin catalytic subunit, an ADP-ribosyltransferase.. (Microbial infection) Plays a key role in the endocytosis of enterovirus 71 and thus viral entry into brain microvascular endothelial cells.

See full target information ADP-ribosylation factor 6

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Publications (6)

Recent publications for all applications. Explore the full list and refine your search

Journal of extracellular biology 3:e139 PubMed38938682

2024

Activation of the receptor KIT induces the secretion of exosome-like small extracellular vesicles.

Applications

Unspecified application

Species

Unspecified reactive species

Annika Pfeiffer,Geethani Bandara,Jennifer D Petersen,Guido H Falduto,Joshua Zimmerberg,Dean D Metcalfe,Ana Olivera

Cell reports 42:112013 PubMed36656711

2023

VGLL2-NCOA2 leverages developmental programs for pediatric sarcomagenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Sarah Watson,Collette A LaVigne,Lin Xu,Didier Surdez,Joanna Cyrta,Delia Calderon,Matthew V Cannon,Matthew R Kent,Katherine M Silvius,Jack P Kucinski,Emma N Harrison,Whitney Murchison,Dinesh Rakheja,Franck Tirode,Olivier Delattre,James F Amatruda,Genevieve C Kendall

International journal of molecular sciences 24: PubMed36614035

2022

HMGA1 Regulates the Expression of Replication-Dependent Histone Genes and Cell-Cycle in Breast Cancer Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Sara Petrosino,Sabrina Pacor,Silvia Pegoraro,Virginia Anna Gazziero,Giulia Canarutto,Silvano Piazza,Guidalberto Manfioletti,Riccardo Sgarra

Journal of extracellular vesicles 11:e12272 PubMed36239715

2022

Selective immunocapture reveals neoplastic human mast cells secrete distinct microvesicle- and exosome-like populations of KIT-containing extracellular vesicles.

Applications

Unspecified application

Species

Unspecified reactive species

Annika Pfeiffer,Jennifer D Petersen,Guido H Falduto,David Eric Anderson,Joshua Zimmerberg,Dean D Metcalfe,Ana Olivera

Experimental and therapeutic medicine 22:1205 PubMed34584550

2021

miR-28-3p inhibits prostate cancer cell proliferation, migration and invasion, and promotes apoptosis by targeting ARF6.

Applications

Unspecified application

Species

Unspecified reactive species

Jiabin Zhang,Yi Yao,Huizhang Li,Shihua Ye

PloS one 15:e0229747 PubMed32119711

2020

Angiotensin II promotes podocyte injury by activating Arf6-Erk1/2-Nox4 signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Guanghua Che,Hang Gao,Qibo Hu,Hongchang Xie,Yunfeng Zhang

View all publications

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Product promise

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For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-ARF6 antibody

Immunoprecipitation - Anti-ARF6 antibody (AB226389)

Western blot - Anti-ARF6 antibody (AB226389)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

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Secondary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (6)

Product promise