AB248394

Anti-ARF6 antibody [EPR8357] - BSA and Azide free

RabMAb
Recombinant
KO Validated
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(1 Publication)

Rabbit Recombinant Monoclonal ARF6 antibody. Carrier free. Suitable for WB and reacts with Human, Mouse, Rat samples. Cited in 1 publication.

View Alternative Names

ADP-ribosylation factor 6, ARF6

5 Images

Lab

Western blot - Anti-ARF6 antibody [EPR8357] - BSA and Azide free (AB248394)

This data was developed using ab131261, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-ARF6 antibody [EPR8357] (<a href='/en-us/products/primary-antibodies/arf6-antibody-epr8357-ab131261'>ab131261</a>) at 1/200 dilution

Lane 1:

MCF7 (Human breast adenocarcinoma epithelial cell) whole cell lysates at 15 µg

Lane 2:

Mouse kidney lysates at 15 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 20 kDa

Observed band size: 18 kDa

false

Unknown

Western blot - Anti-ARF6 antibody [EPR8357] - BSA and Azide free (AB248394)

This data was developed using ab131261, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-ARF6 antibody [EPR8357] (<a href='/en-us/products/primary-antibodies/arf6-antibody-epr8357-ab131261'>ab131261</a>) at 1/1000 dilution

Lane 1:

MCF7 cell lysate at 10 µg

Lane 2:

293T cell lysate at 10 µg

Lane 3:

A431 cell lysate at 10 µg

Predicted band size: 20 kDa

false

Lab

Western blot - Anti-ARF6 antibody [EPR8357] - BSA and Azide free (AB248394)

This data was developed using ab131261, the same antibody clone in a different buffer formulation.

Lane 1 : Wild-type HAP1 cell lysate (20 µg).

Lane 2 : ARF6 knockout HAP1 cell lysate (20 µg).

Lane 3 : HEK293 cell lysate (20 µg).

Lane 4 : HeLa cell lysate (20 µg).

Lanes 1 - 4 : Merged signal (red and green). Green - ab131261 (unpurified) observed at 20 kDa. Red - loading control, ab8245, observed at 37 kDa. ab131261 was shown to recognize ARF6 when ARF6 knockout samples were used, along with additional cross-reactive bands. Wild-type and ARF6 knockout samples were subjected to SDS-PAGE. ab131261 and ab8245 (loading control to GAPDH) were diluted 1/1000 and 1/2000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10000 dilution for 1 h at room temperature before imaging.

All lanes:

Western blot - Anti-ARF6 antibody [EPR8357] (<a href='/en-us/products/primary-antibodies/arf6-antibody-epr8357-ab131261'>ab131261</a>)

Predicted band size: 20 kDa

false

Lab

Western blot - Anti-ARF6 antibody [EPR8357] - BSA and Azide free (AB248394)

This data was developed using ab131261, the same antibody clone in a different buffer formulation.

This antibody shows low affinity on HeLa cells and detects a strong non-specific band around 75 KD.

All lanes:

Western blot - Anti-ARF6 antibody [EPR8357] (<a href='/en-us/products/primary-antibodies/arf6-antibody-epr8357-ab131261'>ab131261</a>) at 1/1000 dilution

All lanes:

HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates at 15 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 20 kDa

Observed band size: 18 kDa

false

Lab

Western blot - Anti-ARF6 antibody [EPR8357] - BSA and Azide free (AB248394)

This data was developed using ab131261, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-ARF6 antibody [EPR8357] (<a href='/en-us/products/primary-antibodies/arf6-antibody-epr8357-ab131261'>ab131261</a>) at 1/1000 dilution

All lanes:

Rat kidney lysates at 15 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 20 kDa

Observed band size: 18 kDa

false

Unconjugated

Anti-ARF6 antibody [EPR8357]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR8357

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Rat, Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Mouse": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Product details

ab248394 is the carrier-free version of ab131261.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The ARF6 protein also known as ADP-ribosylation factor 6 is a small GTPase with a molecular weight of approximately 21 kDa. The protein belongs to the ARF family which plays roles in regulating vesicular trafficking and cytoskeletal dynamics. ARF6 is ubiquitously expressed across many tissues most abundantly in the brain muscle and epithelial cells. Its activity depends on cycling between an inactive GDP-bound form and an active GTP-bound form which is important for its function in cellular processes.

Biological function summary

The activity of ARF6 influences membrane trafficking and actin cytoskeleton remodeling. The protein helps mediate endocytosis and exocytosis as well as modulating cell adhesion and migration. ARF6 interacts with various effector proteins to form a complex that is involved in the coordination of these processes. This complex activity underlines ARF6's roles in cell signaling and intracellular trafficking mechanisms.

Pathways

ARF6 plays key roles in the Wnt signaling pathway and the E-cadherin recycling pathway. In the Wnt pathway ARF6 regulates the internalization of receptors and affects downstream signaling. In E-cadherin recycling ARF6 orchestrates trafficking events that ensure proper cell-cell adhesion. These functions often involve interactions with related proteins like ARF1 and Rac1 which further modulate ARF6's involvement in these signaling events influencing cellular behavior.

ARF6 has a connection to certain cancers and neurological conditions. Its dysregulation can lead to increased metastasis in cancers partly due to altered cell motility and invasion linked to changes in cell adhesion. Additionally the misregulation of ARF6 is implicated in Alzheimer's disease where it may disrupt neuronal trafficking and contribute to synaptic dysfunction. These disease associations also involve related proteins such as EFA6 and ARHGAP10 which can modulate ARF6 activity and its effects on pathological processes.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

GTP-binding protein involved in protein trafficking that regulates endocytic recycling and cytoskeleton remodeling (PubMed : 11266366, PubMed : 16737952, PubMed : 18400762, PubMed : 21170023, PubMed : 32103017, PubMed : 7589240). GTP-bound form plays an important role in the transport of multiple palmitoylated proteins form the Golgi to the plasma membrane (PubMed : 37461827). Required for normal completion of mitotic cytokinesis (By similarity). Plays a role in the reorganization of the actin cytoskeleton and the formation of stress fibers (By similarity). Involved in the regulation of dendritic spine development, contributing to the regulation of dendritic branching and filopodia extension (PubMed : 14978216). Potentiates the neurite outgrowth in primary neurons by interacting with the molecular adapter APBB1 (PubMed : 36250347). Plays an important role in membrane trafficking, during junctional remodeling and epithelial polarization (PubMed : 36017701). Regulates surface levels of adherens junction proteins such as CDH1 (By similarity). Required for NTRK1 sorting to the recycling pathway from early endosomes (By similarity).. (Microbial infection) Functions as an allosteric activator of the cholera toxin catalytic subunit, an ADP-ribosyltransferase.. (Microbial infection) Plays a key role in the endocytosis of enterovirus 71 and thus viral entry into brain microvascular endothelial cells.

See full target information ADP-ribosylation factor 6

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Oncology letters 24:457 PubMed36380881

2022

Suppressive effect of YY1-mediated RGS22 regulation on the proliferation, migration and invasion of pancreatic ductal adenocarcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Shou-Ji Cao,Wan-Li Ge,Ling-Dong Meng,Qun Chen,Yi Miao,Kui-Rong Jiang,Jing-Jing Zhang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-ARF6 antibody [EPR8357] - BSA and Azide free

Western blot - Anti-ARF6 antibody [EPR8357] - BSA and Azide free (AB248394)

Western blot - Anti-ARF6 antibody [EPR8357] - BSA and Azide free (AB248394)

Western blot - Anti-ARF6 antibody [EPR8357] - BSA and Azide free (AB248394)

Western blot - Anti-ARF6 antibody [EPR8357] - BSA and Azide free (AB248394)

Western blot - Anti-ARF6 antibody [EPR8357] - BSA and Azide free (AB248394)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Complementary Products

Primary Antibodies

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Product promise