AB191430

Anti-Arfaptin-1 antibody [EPR16360]

RabMAb
Recombinant
What is this?

Be the first to review this product! Submit a review

(1 Publication)

Rabbit Recombinant Monoclonal Arfaptin-1 antibody. Suitable for WB, Flow Cyt (Intra) and reacts with Mouse, Rat, Human samples. Cited in 1 publication.

View Alternative Names

Arfaptin-1, ADP-ribosylation factor-interacting protein 1, ARFIP1

4 Images

Flow Cytometry (Intracellular) - Anti-Arfaptin-1 antibody [EPR16360] (AB191430)

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-Arfaptin-1 antibody [EPR16360] (AB191430)

Intracellular flow cytometrical analysis of Jurkat cells labeling Arfaptin-1 with ab191430 at 1/120 compared to a Isotype control. FITC-conjugated goat-anti-rabbit secondary antibody at 1/150 were used for the analysis.

Supplier Data

Western blot - Anti-Arfaptin-1 antibody [EPR16360] (AB191430)

All lanes:

Western blot - Anti-Arfaptin-1 antibody [EPR16360] (ab191430) at 1/10000 dilution

Lane 1:

HeLa cell lysate at 20 µg

Lane 2:

HepG2 cell lysate at 20 µg

Lane 3:

BxPC-3 cell lysate at 20 µg

Lane 4:

Jurkat cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 42 kDa

Observed band size: 44 kDa

false

Supplier Data

Western blot - Anti-Arfaptin-1 antibody [EPR16360] (AB191430)

All lanes:

Western blot - Anti-Arfaptin-1 antibody [EPR16360] (ab191430) at 1/10000 dilution

All lanes:

Human fetal liver tissue lysate at 10 µg

Secondary

All lanes:

Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 42 kDa

Observed band size: 44 kDa

false

Supplier Data

Western blot - Anti-Arfaptin-1 antibody [EPR16360] (AB191430)

All lanes:

Western blot - Anti-Arfaptin-1 antibody [EPR16360] (ab191430) at 1/1000 dilution

Lane 1:

Mouse brain lysate at 10 µg

Lane 2:

Rat brain lysate at 10 µg

Lane 3:

Raw264.7 lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

Predicted band size: 42 kDa

Observed band size: 44 kDa

false

Carrier free

Anti-Arfaptin-1 antibody [EPR16360] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR16360

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

WB, Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/120", "FlowCytIntra-species-notes": "<a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody." }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

style

left-column

Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Arfaptin-1 also known as ARFIP1 acts mechanically by modulating membrane dynamics and cell signaling. This protein with a mass of approximately 36 kDa associates with membranes through a BAR domain which induces curvature. It interacts with small GTPases including ARF and Rac1 to facilitate various intracellular trafficking processes. Arfaptin-1 expression occurs in multiple tissue types suggesting its wide role in cellular functions.

Biological function summary

Arfaptin-1 plays a part in intracellular transport and membrane receptor regulation. It is also recognized as a component of larger protein complexes contributing to the reorganization of actin cytoskeleton. Through its interactions with GTPases Arfaptin-1 influences endocytosis and exocytosis affecting cell surface receptor turnover. This adaptability allows it to play important roles in cellular communication and response to environmental stimuli.

Pathways

Arfaptin-1 is integral in cellular trafficking and signal transduction pathways. It is involved in the ARF small GTPase pathway influencing vesicle formation and trafficking. Arfaptin-1 also interacts with other proteins like Rac1 in the regulation of actin dynamics impacting the actin cytoskeleton remodeling pathway. These interactions highlight its central position in coordinating cellular architecture and material transport.

Arfaptin-1 involvement links to conditions like cancer and neurological disorders. Altered expression of Arfaptin-1 has been observed in cancer cells where it affects cell migration and invasion linking with other proteins such as Rac1. In neurological disorders abnormal Arfaptin-1 function disrupts synaptic vesicle trafficking potentially interacting with proteins involved in neurodegenerative diseases. These associations emphasize its potential as a target in therapeutic strategies.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Plays a role in controlling biogenesis of secretory granules at the trans-Golgi network (PubMed : 22981988). Mechanistically, binds ARF-GTP at the neck of a growing secretory granule precursor and forms a protective scaffold (PubMed : 22981988, PubMed : 9038142). Once the granule precursor has been completely loaded, active PRKD1 phosphorylates ARFIP1 and releases it from ARFs (PubMed : 22981988). In turn, ARFs induce fission (PubMed : 22981988). Through this mechanism, ensures proper secretory granule formation at the Golgi of pancreatic beta cells (PubMed : 22981988).

See full target information ARFIP1

style

left-column

Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Endocrinology 158:3778-3791 PubMed28973134

2017

MEF2C Interacts With c-FOS in PTH-Stimulated Mmp13 Gene Expression in Osteoblastic Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Teruyo Nakatani,Nicola C Partridge

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Arfaptin-1 antibody [EPR16360]

Flow Cytometry (Intracellular) - Anti-Arfaptin-1 antibody [EPR16360] (AB191430)

Western blot - Anti-Arfaptin-1 antibody [EPR16360] (AB191430)

Western blot - Anti-Arfaptin-1 antibody [EPR16360] (AB191430)

Western blot - Anti-Arfaptin-1 antibody [EPR16360] (AB191430)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Product promise