AB204405

Anti-ARFGAP1 antibody

Name: Anti-ARFGAP1 antibody (ab204405) | Abcam
Brand: Abcam Limited
SKU: AB204405
Price: 510 USD
Availability: InStock

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(5 Publications)

Rabbit Polyclonal ARFGAP1 antibody. Suitable for IHC-P, ICC, WB and reacts with Human samples. Cited in 5 publications. Immunogen corresponding to Recombinant Fragment Protein within Human ARFGAP1 aa 100-250.

View Alternative Names

ARF1GAP, ARFGAP1, ADP-ribosylation factor GTPase-activating protein 1, ARF GAP 1, ADP-ribosylation factor 1 GTPase-activating protein, ARF1-directed GTPase-activating protein, ARF1 GAP

6 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARFGAP1 antibody (AB204405)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARFGAP1 antibody (AB204405)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human skeletal muscle tissue labelling ARFGAP1 with ab204405 at 1/1000 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARFGAP1 antibody (AB204405)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human pancreas tissue labelling ARFGAP1 with ab204405 at 1/1000 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARFGAP1 antibody (AB204405)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human tonsil tissue labelling ARFGAP1 with ab204405 at 1/1000 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARFGAP1 antibody (AB204405)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human placenta tissue labelling ARFGAP1 with ab204405 at 1/1000 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.

Supplier Data

Immunocytochemistry - Anti-ARFGAP1 antibody (AB204405)

Immunofluorescent analysis of CACO-2 cells labeling ARFGAP1 with ab204405 at 4 μg/mL. Fixation/Permeabilization : PFA/Triton X-100.

Supplier Data

Western blot - Anti-ARFGAP1 antibody (AB204405)

All lanes:

Western blot - Anti-ARFGAP1 antibody (ab204405) at 0.4 µg/mL

Lane 1:

RT4 (human urinary bladder cancer cell line) cell lysate

Lane 2:

U-251 MG (formally U-373 MG) (human brain glioma cell line) whole cell lysate

Predicted band size: 45 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IHC-P, WB, ICC

applications

Immunogen

Recombinant Fragment Protein within Human ARFGAP1 aa 100-250. The exact immunogen used to generate this antibody is proprietary information.

Q8N6T3

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICC" : {"fullname" : "Immunocytochemistry", "shortname":"ICC"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/1000 - 1/2500", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "ICC-species-checked": "testedAndGuaranteed", "ICC-species-dilution-info": "0.25-2 µg/mL", "ICC-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ARFGAP1 also known as ADP-ribosylation factor GTPase-activating protein 1 operates mechanically to regulate the activity of ARF family proteins by enhancing GTP hydrolysis. Its enzymatic role involves facilitating ARF in their active and inactive states performing critical functions in vesicular trafficking and membrane dynamics. ARFGAP1 has a molecular weight of approximately 50 kDa and shows expression in various tissues where it particularly functions with intracellular membranes linked to the Golgi apparatus.

Biological function summary

ARFGAP1 plays a significant role in intracellular trafficking and its activity forms an essential part of the COPI coat protein complex affecting vesicle formation and transport across the Golgi. This complex collaborates closely with ARFs to maintain cellular cargo trafficking and organelle structure integrity. Efficient functioning of ARFGAP1 is necessary for proper protein transport within the cell reflecting its participation in essential cellular processes.

Pathways

Several significant cellular processes involve ARFGAP1 through its contribution to the vesicular transport and endocytosis pathways. Within these pathways ARFGAP1 coordinates with proteins like ARF1 which is important for vesicle budding and cargo selection. It ensures the accurate conveyance and distribution of proteins and lipids impacting numerous cellular mechanisms and their regulatory controls.

Improper ARFGAP1 function associates with conditions like cancer and neurological disorders. Its dysregulation can affect cellular transport processes thereby influencing disease progression. In cancer altered ARFGAP1 activity interacts with proteins such as ARF1 potentially contributing to abnormal cell division and metastasis. Its involvement in neurological disorders is linked to disruptions in membrane trafficking affecting neuron communication and function.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

GTPase-activating protein (GAP) for the ADP ribosylation factor 1 (ARF1). Involved in membrane trafficking and /or vesicle transport. Promotes hydrolysis of the ARF1-bound GTP and thus, is required for the dissociation of coat proteins from Golgi-derived membranes and vesicles, a prerequisite for vesicle's fusion with target compartment. Probably regulates ARF1-mediated transport via its interaction with the KDELR proteins and TMED2. Overexpression induces the redistribution of the entire Golgi complex to the endoplasmic reticulum, as when ARF1 is deactivated. Its activity is stimulated by phosphoinosides and inhibited by phosphatidylcholine (By similarity).

See full target information ARFGAP1

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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Cell communication and signaling : CCS 22:140 PubMed38378560

2024

Crosstalk between KDEL receptor and EGF receptor mediates cell proliferation and migration via STAT3 signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Jie Jia,Lianhui Zhu,Xihua Yue,Shuocheng Tang,Shuaiyang Jing,Chuanting Tan,Yulei Du,Jingkai Gao,Intaek Lee,Yi Qian

Cells 12: PubMed37048152

2023

KDEL Receptor Trafficking to the Plasma Membrane Is Regulated by ACBD3 and Rab4A-GTP.

Applications

Unspecified application

Species

Unspecified reactive species

Chuanting Tan,Yulei Du,Lianhui Zhu,Shuaiyang Jing,Jingkai Gao,Yi Qian,Xihua Yue,Intaek Lee

Communications biology 4:1370 PubMed34876695

2021

Tankyrase-1-mediated degradation of Golgin45 regulates glycosyltransferase trafficking and protein glycosylation in Rab2-GTP-dependent manner.

Applications

Unspecified application

Species

Unspecified reactive species

Xihua Yue,Neeraj Tiwari,Lianhui Zhu,Hai Dang Truong Ngo,Jae-Min Lim,Bopil Gim,Shuaiyang Jing,Yijing Wang,Yi Qian,Intaek Lee

BMC biology 19:194 PubMed34493279

2021

ACBD3 modulates KDEL receptor interaction with PKA for its trafficking via tubulovesicular carrier.

Applications

Unspecified application

Species

Unspecified reactive species

Xihua Yue,Yi Qian,Lianhui Zhu,Bopil Gim,Mengjing Bao,Jie Jia,Shuaiyang Jing,Yijing Wang,Chuanting Tan,Francesca Bottanelli,Pascal Ziltener,Sunkyu Choi,Piliang Hao,Intaek Lee

Nature communications 12:3428 PubMed34103526

2021

Low chorionic villous succinate accumulation associates with recurrent spontaneous abortion risk.

Applications

Unspecified application

Species

Unspecified reactive species

Xiao-Hui Wang,Sha Xu,Xiang-Yu Zhou,Rui Zhao,Yan Lin,Jing Cao,Wei-Dong Zang,Hui Tao,Wei Xu,Ming-Qing Li,Shi-Min Zhao,Li-Ping Jin,Jian-Yuan Zhao

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-ARFGAP1 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARFGAP1 antibody (AB204405)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARFGAP1 antibody (AB204405)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARFGAP1 antibody (AB204405)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARFGAP1 antibody (AB204405)

Immunocytochemistry - Anti-ARFGAP1 antibody (AB204405)

Western blot - Anti-ARFGAP1 antibody (AB204405)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (5)

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