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AB250706

Anti-ARFGAP1 antibody [EPR13650] - BSA and Azide free

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(1 Publication)

Rabbit Recombinant Monoclonal ARFGAP1 antibody. Carrier free. Suitable for ICC/IF, WB and reacts with Human samples. Cited in 1 publication.

View Alternative Names

ARF1GAP, ARFGAP1, ADP-ribosylation factor GTPase-activating protein 1, ARF GAP 1, ADP-ribosylation factor 1 GTPase-activating protein, ARF1-directed GTPase-activating protein, ARF1 GAP

4 Images
Immunocytochemistry/ Immunofluorescence - Anti-ARFGAP1 antibody [EPR13650] - BSA and Azide free (AB250706)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-ARFGAP1 antibody [EPR13650] - BSA and Azide free (AB250706)

This data was developed using ab183746, the same antibody clone in a different buffer formulation.

Immunofluorescent analysis of 4% paraformaldehyde-fixed MCF7 cells labeling ARFGAP1 with ab183746 at 1/500 dilution followed by Goat anti rabbit IgG (Alexa Fluor®555) secondary antibody at 1/200 dilution (red). Counter stained with Dapi (blue).

Immunocytochemistry/ Immunofluorescence - Anti-ARFGAP1 antibody [EPR13650] - BSA and Azide free (AB250706)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-ARFGAP1 antibody [EPR13650] - BSA and Azide free (AB250706)

This data was developed using ab183746, the same antibody clone in a different buffer formulation.

Immunofluorescent analysis of acetone-fixed HeLa cells labeling ARFGAP1 with ab183746 at 1/500 dilution followed by Goat anti rabbit IgG (Alexa Fluor®555) secondary antibody at 1/200 dilution (red). Counter stained with Dapi (blue).

Western blot - Anti-ARFGAP1 antibody [EPR13650] - BSA and Azide free (AB250706)
  • WB

Supplier Data

Western blot - Anti-ARFGAP1 antibody [EPR13650] - BSA and Azide free (AB250706)

This data was developed using ab183746, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-ARFGAP1 antibody [EPR13650] (<a href='/en-us/products/primary-antibodies/arfgap1-antibody-epr13650-ab183746'>ab183746</a>) at 1/1000 dilution

Lane 1:

HeLa cell lysate at 20 µg

Lane 2:

MCF7 cell lysate at 20 µg

Secondary

All lanes:

Goat anti-rabbit IgG, (H+L), peroxidase conjugate at 1/1000 dilution

Predicted band size: 45 kDa

Observed band size: 45 kDa

false

Western blot - Anti-ARFGAP1 antibody [EPR13650] - BSA and Azide free (AB250706)
  • WB

Supplier Data

Western blot - Anti-ARFGAP1 antibody [EPR13650] - BSA and Azide free (AB250706)

This data was developed using ab183746, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-ARFGAP1 antibody [EPR13650] (<a href='/en-us/products/primary-antibodies/arfgap1-antibody-epr13650-ab183746'>ab183746</a>) at 1/5000 dilution

Lane 1:

Human fetal brain tissue lysate at 20 µg

Lane 2:

HepG2 cell lysate at 20 µg

Secondary

All lanes:

Goat anti-rabbit IgG, (H+L), peroxidase conjugate at 1/1000 dilution

Predicted band size: 45 kDa

Observed band size: 45 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR13650

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

ICC/IF, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" }, "Mouse": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Product details

ab250706 is the carrier-free version of ab183746.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ARFGAP1 also known as ADP-ribosylation factor GTPase-activating protein 1 operates mechanically to regulate the activity of ARF family proteins by enhancing GTP hydrolysis. Its enzymatic role involves facilitating ARF in their active and inactive states performing critical functions in vesicular trafficking and membrane dynamics. ARFGAP1 has a molecular weight of approximately 50 kDa and shows expression in various tissues where it particularly functions with intracellular membranes linked to the Golgi apparatus.
Biological function summary

ARFGAP1 plays a significant role in intracellular trafficking and its activity forms an essential part of the COPI coat protein complex affecting vesicle formation and transport across the Golgi. This complex collaborates closely with ARFs to maintain cellular cargo trafficking and organelle structure integrity. Efficient functioning of ARFGAP1 is necessary for proper protein transport within the cell reflecting its participation in essential cellular processes.

Pathways

Several significant cellular processes involve ARFGAP1 through its contribution to the vesicular transport and endocytosis pathways. Within these pathways ARFGAP1 coordinates with proteins like ARF1 which is important for vesicle budding and cargo selection. It ensures the accurate conveyance and distribution of proteins and lipids impacting numerous cellular mechanisms and their regulatory controls.

Improper ARFGAP1 function associates with conditions like cancer and neurological disorders. Its dysregulation can affect cellular transport processes thereby influencing disease progression. In cancer altered ARFGAP1 activity interacts with proteins such as ARF1 potentially contributing to abnormal cell division and metastasis. Its involvement in neurological disorders is linked to disruptions in membrane trafficking affecting neuron communication and function.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

GTPase-activating protein (GAP) for the ADP ribosylation factor 1 (ARF1). Involved in membrane trafficking and /or vesicle transport. Promotes hydrolysis of the ARF1-bound GTP and thus, is required for the dissociation of coat proteins from Golgi-derived membranes and vesicles, a prerequisite for vesicle's fusion with target compartment. Probably regulates ARF1-mediated transport via its interaction with the KDELR proteins and TMED2. Overexpression induces the redistribution of the entire Golgi complex to the endoplasmic reticulum, as when ARF1 is deactivated. Its activity is stimulated by phosphoinosides and inhibited by phosphatidylcholine (By similarity).
See full target information ARFGAP1
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

JCI insight 5: PubMed31094704

2019

Protective role of B cells in sterile particulate-induced lung injury.

Applications

Unspecified application

Species

Unspecified reactive species

Shaikh M Atif,Douglas G Mack,Amy S McKee,Javier Rangel-Moreno,Allison K Martin,Andrew Getahun,Lisa A Maier,John C Cambier,Rubin Tuder,Andrew P Fontenot
View all publications
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Product promise

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For full details, please see our Terms & Conditions

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