AB72420

Anti-ARFGEF2/BIG2 antibody

Name: Anti-ARFGEF2/BIG2 antibody (ab72420) | Abcam
Brand: Abcam Limited
SKU: AB72420
Price: 520 USD
Availability: InStock
Rating: 5 (2 reviews)

(2 Reviews)

(1 Publication)

Rabbit Polyclonal ARFGEF2/BIG2 antibody. Suitable for IHC-P, IP, WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human ARFGEF2.

View Alternative Names

ARFGEP2, BIG2, ARFGEF2, Brefeldin A-inhibited guanine nucleotide-exchange protein 2, Brefeldin A-inhibited GEP 2, ADP-ribosylation factor guanine nucleotide-exchange factor 2

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARFGEF2/BIG2 antibody (AB72420)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARFGEF2/BIG2 antibody (AB72420)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human prostate carcinoma tissue labelling ARFGEF2 with ab72420 at 1/200 (1µg/ml). Detection : DAB.

Unknown

Immunoprecipitation - Anti-ARFGEF2/BIG2 antibody (AB72420)

Detection of ARFGEF2/BIG by Western Blot of Immunprecipitate.
ab72420 at 1μg/ml staining ARFGEF2/BIG in HeLa whole cell lysate immunoprecipitated using ab72420 at 3μg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Detection : Chemiluminescence with exposure time of 30 seconds.

All lanes:

Immunoprecipitation - Anti-ARFGEF2/BIG2 antibody (ab72420)

Predicted band size: 202 kDa

false

Unknown

Western blot - Anti-ARFGEF2/BIG2 antibody (AB72420)

All lanes:

Western blot - Anti-ARFGEF2/BIG2 antibody (ab72420) at 0.04 µg/mL

Lane 1:

HeLa whole cell lysate at 50 µg

Lane 2:

HeLa whole cell lysate at 15 µg

Predicted band size: 202 kDa

Observed band size: 202 kDa

true

Exposure time: 3min

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, IHC-P, IP

applications

Immunogen

Synthetic Peptide within Human ARFGEF2. The exact immunogen used to generate this antibody is proprietary information.

Q9Y6D5

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/500", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-5 µg/mg of lysate", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "" }, "Chimpanzee": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Gorilla": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rhesus monkey": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

ab72420 was affinity purified using an epitope specific to ARFGEF2/BIG2 immobilized on solid support.

Storage buffer

pH: 6.8 - 7.4 Preservative: 0.09% Sodium azide Constituents: Tris buffered saline, 0.1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ARFGEF2 also known as BIG2 is a guanine nucleotide exchange factor (GEF) that regulates the activation of ADP-ribosylation factors (ARFs). This protein helps in the exchange of GDP to GTP on ARFs which is essential for the function of various cellular processes involving membrane trafficking. ARFGEF2 has a molecular mass of approximately 206 kDa. It is predominantly expressed in the brain particularly in the neurons but also shows expression in other tissues including heart and kidney.

Biological function summary

ARFGEF2/BIG2 is important in maintaining proper Golgi apparatus function and vesicle trafficking. It forms part of various complexes related to vesicle formation and transport within the neuron and other cells. Through its activity ARFGEF2 influences the organization of the Golgi and modulates protein sorting and export. Consequently this protein plays an important role in neural development and cell signaling.

Pathways

ARFGEF2/BIG2 participates actively in the secretory pathway and is a significant player in the trafficking of vesicles from the Golgi apparatus. The protein works closely with ARF proteins facilitating vesicle budding and cargo sorting. Additionally ARFGEF2 interacts with coat protein complex I (COPI) highlighting its role in retrograde transport from the Golgi to the endoplasmic reticulum. It also functions in concert with regulators of membrane curvature to enable proper vesicle formation.

ARFGEF2/BIG2 is linked to the condition known as periventricular heterotopia with microcephaly (PHM) a disorder affecting brain development. Mutations in this protein lead to problems in vesicular trafficking and neuronal migration. Furthermore the improper function of ARFGEF2 can impact the RhoA signaling pathway connected to schizophrenia where RhoA proteins are important linked elements. Defective or altered ARFGEF2 may result in compromised neural structures and predispose individuals to such neurological conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Promotes guanine-nucleotide exchange on ARF1 and ARF3 and to a lower extent on ARF5 and ARF6. Promotes the activation of ARF1/ARF5/ARF6 through replacement of GDP with GTP. Involved in the regulation of Golgi vesicular transport. Required for the integrity of the endosomal compartment. Involved in trafficking from the trans-Golgi network (TGN) to endosomes and is required for membrane association of the AP-1 complex and GGA1. Seems to be involved in recycling of the transferrin receptor from recycling endosomes to the plasma membrane. Probably is involved in the exit of GABA(A) receptors from the endoplasmic reticulum. Involved in constitutive release of tumor necrosis factor receptor 1 via exosome-like vesicles; the function seems to involve PKA and specifically PRKAR2B. Proposed to act as A kinase-anchoring protein (AKAP) and may mediate crosstalk between Arf and PKA pathways.

See full target information ARFGEF2

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Cell death & disease 16:270 PubMed40204721

2025

USP10 stabilizes BAZ1A to drive tumor stemness via an epigenetic mechanism in head and neck squamous cell carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Yanni Shi,Jiawei Ding,Xiao Ling,Danfeng Xu,Yan Shen,Xingjun Qin

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-ARFGEF2/BIG2 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARFGEF2/BIG2 antibody (AB72420)

Immunoprecipitation - Anti-ARFGEF2/BIG2 antibody (AB72420)

Western blot - Anti-ARFGEF2/BIG2 antibody (AB72420)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

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