AB250188

Anti-ARFGEF2/BIG2 antibody [EPR10049] - BSA and Azide free

RabMAb
Recombinant
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(1 Publication)

Rabbit Recombinant Monoclonal ARFGEF2/BIG2 antibody. Carrier free. Suitable for WB and reacts with Human samples. Cited in 1 publication.

View Alternative Names

ARFGEP2, BIG2, ARFGEF2, Brefeldin A-inhibited guanine nucleotide-exchange protein 2, Brefeldin A-inhibited GEP 2, ADP-ribosylation factor guanine nucleotide-exchange factor 2

1 Images

Supplier Data

Western blot - Anti-ARFGEF2/BIG2 antibody [EPR10049] - BSA and Azide free (AB250188)

This data was developed using ab180186, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-ARFGEF2/BIG2 antibody [EPR10049] (<a href='/en-us/products/primary-antibodies/arfgef2-big2-antibody-epr10049-ab180186'>ab180186</a>) at 1/1000 dilution

Lane 1:

HeLa cell lysate at 10 µg

Lane 2:

HepG2 cell lysate at 10 µg

Lane 3:

293T cell lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 202 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR10049

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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Product details

ab250188 is the carrier-free version of ab180186.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ARFGEF2 also known as BIG2 is a guanine nucleotide exchange factor (GEF) that regulates the activation of ADP-ribosylation factors (ARFs). This protein helps in the exchange of GDP to GTP on ARFs which is essential for the function of various cellular processes involving membrane trafficking. ARFGEF2 has a molecular mass of approximately 206 kDa. It is predominantly expressed in the brain particularly in the neurons but also shows expression in other tissues including heart and kidney.

Biological function summary

ARFGEF2/BIG2 is important in maintaining proper Golgi apparatus function and vesicle trafficking. It forms part of various complexes related to vesicle formation and transport within the neuron and other cells. Through its activity ARFGEF2 influences the organization of the Golgi and modulates protein sorting and export. Consequently this protein plays an important role in neural development and cell signaling.

Pathways

ARFGEF2/BIG2 participates actively in the secretory pathway and is a significant player in the trafficking of vesicles from the Golgi apparatus. The protein works closely with ARF proteins facilitating vesicle budding and cargo sorting. Additionally ARFGEF2 interacts with coat protein complex I (COPI) highlighting its role in retrograde transport from the Golgi to the endoplasmic reticulum. It also functions in concert with regulators of membrane curvature to enable proper vesicle formation.

ARFGEF2/BIG2 is linked to the condition known as periventricular heterotopia with microcephaly (PHM) a disorder affecting brain development. Mutations in this protein lead to problems in vesicular trafficking and neuronal migration. Furthermore the improper function of ARFGEF2 can impact the RhoA signaling pathway connected to schizophrenia where RhoA proteins are important linked elements. Defective or altered ARFGEF2 may result in compromised neural structures and predispose individuals to such neurological conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Promotes guanine-nucleotide exchange on ARF1 and ARF3 and to a lower extent on ARF5 and ARF6. Promotes the activation of ARF1/ARF5/ARF6 through replacement of GDP with GTP. Involved in the regulation of Golgi vesicular transport. Required for the integrity of the endosomal compartment. Involved in trafficking from the trans-Golgi network (TGN) to endosomes and is required for membrane association of the AP-1 complex and GGA1. Seems to be involved in recycling of the transferrin receptor from recycling endosomes to the plasma membrane. Probably is involved in the exit of GABA(A) receptors from the endoplasmic reticulum. Involved in constitutive release of tumor necrosis factor receptor 1 via exosome-like vesicles; the function seems to involve PKA and specifically PRKAR2B. Proposed to act as A kinase-anchoring protein (AKAP) and may mediate crosstalk between Arf and PKA pathways.

See full target information ARFGEF2

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Experimental and therapeutic medicine 23:157 PubMed35069838

2022

APPL1 ameliorates myocardial ischemia-reperfusion injury by regulating the AMPK signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Yunguang Cen,Wei Liao,Taihao Wang,Daimin Zhang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-ARFGEF2/BIG2 antibody [EPR10049] - BSA and Azide free

Western blot - Anti-ARFGEF2/BIG2 antibody [EPR10049] - BSA and Azide free (AB250188)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

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Product promise