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AB108199

Anti-ARFRP1/ARP antibody [EPR3899]

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(3 Publications)

Rabbit Recombinant Monoclonal ARFRP1/ARP antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 3 publications.

View Alternative Names

ARP1, ARFRP1, ADP-ribosylation factor-related protein 1, ARF-related protein 1, ARP

2 Images
Immunocytochemistry/ Immunofluorescence - Anti-ARFRP1/ARP antibody [EPR3899] (AB108199)
  • ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-ARFRP1/ARP antibody [EPR3899] (AB108199)

Immunocytochemistry/ Immunofluorescence analysis of HeLa (human cervix adenocarcinoma epithelial cell) cells labeling ARFRP1/ARP with purified ab108199 at 1/500 dilution (4.8 μg/mL). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) at 1/200 (2.5 μg/mL). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 μg/mL) dilution. DAPI (blue) was used as nuclear counterstain. ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 μg/mL) was used as the secondary antibody only control.

Western blot - Anti-ARFRP1/ARP antibody [EPR3899] (AB108199)
  • WB

Unknown

Western blot - Anti-ARFRP1/ARP antibody [EPR3899] (AB108199)

All lanes:

Western blot - Anti-ARFRP1/ARP antibody [EPR3899] (ab108199) at 1/1000 dilution

Lane 1:

HepG2 (Human liver hepatocellular carcinoma cell line) cell lysate at 10 µg

Lane 2:

HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate at 10 µg

Lane 3:

Fetal liver lysate at 10 µg

Lane 4:

Ovary cancer lysate at 10 µg

Predicted band size: 23 kDa

false

  • Carrier free

    Anti-ARFRP1/ARP antibody [EPR3899] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR3899

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/500", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Conditional Ambient
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Stable for 12 months at -20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The target ARFRP1 also known as ARP functions mechanistically as a small GTPase belonging to the ARF family. This protein possesses an approximate molecular mass of 20 kDa. It is ubiquitously expressed in various tissues exhibiting a significant presence in the cytoplasm and membrane compartments. ARFRP1 plays a role in regulating membrane trafficking and lipid metabolism influencing the formation and function of vesicles within the cell.
Biological function summary

ARFRP1 participates in intracellular vesicle transport and is critical for maintaining cellular homeostasis. It forms part of a complex that interacts with other proteins involved in vesicular traffic affecting cargo selection and vesicle formation. ARFRP1 contributes to maintaining the structural integrity of the Golgi apparatus and endosomes influencing the distribution of proteins and lipids across cellular compartments.

Pathways

ARFRP1 features prominently in pathways related to endocytic and exocytic transport. In the context of membrane traffic it closely interacts with proteins such as ARL1 and ARL4 which also play roles in these pathways. ARFRP1 influences the functioning of these pathways contributing to both protein recycling and secretion processes.

The dysfunction of ARFRP1 associates with Charcot-Marie-Tooth disease and Bardet-Biedl syndrome. In these conditions ARFRP1 may interact with related proteins like ARF6 impacting cell signaling and membrane dynamics. These interactions can lead to defects in cell transport mechanisms further elucidating the role of ARFRP1 in pathological states.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Trans-Golgi-associated GTPase that regulates protein sorting. Controls the targeting of ARL1 and its effector to the trans-Golgi. Required for the lipidation of chylomicrons in the intestine and required for VLDL lipidation in the liver.
See full target information ARFRP1
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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Molecular metabolism 45:101151 PubMed33359402

2020

The ARFRP1-dependent Golgi scaffolding protein GOPC is required for insulin secretion from pancreatic β-cells.

Applications

Unspecified application

Species

Unspecified reactive species

Ilka Wilhelmi,Stephan Grunwald,Niclas Gimber,Oliver Popp,Gunnar Dittmar,Anup Arumughan,Erich E Wanker,Thomas Laeger,Jan Schmoranzer,Oliver Daumke,Annette Schürmann

Molecular metabolism 8:167-179 PubMed29203237

2017

Adiponectin release and insulin receptor targeting share trans-Golgi-dependent endosomal trafficking routes.

Applications

ICC/IF, WB

Species

Human, Human

Maria Rödiger,Martin W Werno,Ilka Wilhelmi,Christian Baumeier,Deike Hesse,Nina Wettschureck,Stefan Offermanns,Kyungyeun Song,Michael Krauß,Annette Schürmann

PloS one 9:e91675 PubMed24632837

2014

Skeletal muscle perilipin 3 and coatomer proteins are increased following exercise and are associated with fat oxidation.

Applications

WB

Species

Human

Jeffrey D Covington,Jose E Galgani,Cedric Moro,Jamie M LaGrange,Zhengyu Zhang,Arild C Rustan,Eric Ravussin,Sudip Bajpeyi
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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