Rabbit Polyclonal Arg2 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human, Mouse samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human ARG2.
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- Cell death & disease 14:6612023Calbindin 2-specific deletion of arginase 2 preserves visual function after optic nerve crush.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSyed A H Zaidi,Zhimin Xu,Tahira Lemtalsi,Porsche Sandow,Sruthi Athota,Fang Liu,Stephen Haigh,Yuqing Huo,S Priya Narayanan,David J R Fulton,Modesto A Rojas,Abdelrahman Y Fouda,Robert W Caldwell,Ruth B CaldwellPubMed 37816735
- Cellular and molecular life sciences : CMLS 79:412021Acute hypoxia elevates arginase 2 and induces polyamine stress response in zebrafish via evolutionarily conserved mechanism.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBodhisattwa Banerjee,Iryna Khrystoforova,Baruh Polis,Inbar Ben Zvi,David KarasikPubMed 34913090
- Journal of clinical medicine 9:2020Role of Arginase 2 in Murine Retinopathy Associated with Western Diet-Induced Obesity.Applications:Unspecified applicationReactive species:Unspecified reactive speciesReem T Atawia et. al.PubMed 31979105
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 79.99% PBS, 20% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human ARG2. The exact immunogen used to generate this antibody is proprietary information. Database link P78540
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Reactivity data
Select an application| WB | IHC-P | ICC/IF | |
|---|---|---|---|
| Human | Tested | Expected | Tested |
| Mouse | Expected | Tested | Expected |
| Cow | Predicted | Predicted | Predicted |
| Rhesus monkey | Predicted | Predicted | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500 - 1/3000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Cow, Rhesus monkey | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/100 - 1/1000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Cow, Rhesus monkey | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100 - 1/1000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Cow, Rhesus monkey | Dilution info - | Notes - |
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Target data
Function
May play a role in the regulation of extra-urea cycle arginine metabolism and also in down-regulation of nitric oxide synthesis. Extrahepatic arginase functions to regulate L-arginine bioavailability to nitric oxid synthase (NOS). Arginine metabolism is a critical regulator of innate and adaptive immune responses. Seems to be involved in negative regulation of the survival capacity of activated CD4(+) and CD8(+) T cells (PubMed:27745970). May suppress inflammation-related signaling in asthmatic airway epithelium (PubMed:27214549). May contribute to the immune evasion of H.pylori by restricting M1 macrophage activation and polyamine metabolism (By similarity). In fetal dendritic cells may play a role in promoting immune suppression and T cell TNF-alpha production during gestation (PubMed:28614294). Regulates RPS6KB1 signaling, which promotes endothelial cell senescence and inflammation and implicates NOS3/eNOS dysfunction (PubMed:22928666). Can inhibit endothelial autophagy independently of its enzymatic activity implicating mTORC2 signaling (PubMed:25484082). Involved in vascular smooth muscle cell senescence and apoptosis independently of its enzymatic activity (PubMed:23832324). Since NOS is found in the penile corpus cavernosum smooth muscle, the clitoral corpus cavernosum and the vagina, arginase-2 plays a role in both male and female sexual arousal (PubMed:12859189).
Alternative names
Arginase II, Kidney-type arginase, Non-hepatic arginase, Type II arginase, ARG2
Recommended products
Rabbit Polyclonal Arg2 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human, Mouse samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human ARG2.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 79.99% PBS, 20% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human ARG2. The exact immunogen used to generate this antibody is proprietary information. Database link P78540
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Arginase 2 (Arg2) also known as AII is an enzyme with a molecular weight of approximately 39 kDa. It is part of the urea cycle and functions to hydrolyze L-arginine into L-ornithine and urea. Arg2 is a mitochondrial enzyme mainly expressed in the liver kidney and prostate. Its expression can extend to other tissues such as the small intestine in some conditions and its activity is important in regulating arginine availability for various physiological processes.
- Biological function summary
Arg2 plays a role in nitrogen disposal and polyamine synthesis. As part of an enzymatic complex it contributes to the urea cycle which is important for the removal of excess nitrogen from the body. Arginase activity also influences the balance of nitric oxide synthesis as it competes with nitric oxide synthase for their common substrate L-arginine. This competition affects cellular functions which rely on nitric oxide signaling for developing proper vascular and immune responses.
- Pathways
Arg2 is an important player in the urea cycle and the polyamine synthesis pathway. It works alongside the enzymes involved in these processes such as ornithine decarboxylase. Additionally Arg2 has a role in the nitric oxide regulation pathway modulating nitric oxide levels by limiting the availability of L-arginine for nitric oxide synthase. This makes Arg2 essential for metabolic processes and cellular communication.
- Associated diseases and disorders
Altered Arg2 expression has been associated with cardiovascular diseases and renal complications. For instance disruptions in Arg2 function can lead to endothelial dysfunction influencing conditions like atherosclerosis. Arg2 imbalances are also linked to kidney diseases possibly due to its critical role in ammonia detoxification and renal arginine metabolism. Its interaction with other proteins such as nitric oxide synthase further emphasizes its relevance in these health conditions.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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6 product images
Western blot - Anti-Arg2 antibody (ab228700)
10% SDS-PAGE
All lanes: Western blot - Anti-Arg2 antibody (ab228700) at 1/1000 dilution
Lane 1: NT2/D2 (human carcinoma cell line from metastatic lung) whole cell lysate at 30 µg
Lane 2: PC-3 (human prostate adenocarcinoma cell line) whole cell lysate at 30 µg
Developed using the ECL technique.
Predicted band size: 39 kDa
Immunocytochemistry/ Immunofluorescence - Anti-Arg2 antibody (ab228700)
Ice-cold methanol-fixed HepG2 (human liver hepatocellular carcinoma cell line) cells stained for Arg2 (green) using ab228700 at 1/500 dilution in ICC/IF.
Nuclear counterstain: Hoechst 33342 (blue).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Arg2 antibody (ab228700)
Paraffin-embedded mouse kidney tissue stained for Arg2 with ab228700 at 1/500 dilution in immunohistochemical analysis.
Western blot - Anti-Arg2 antibody (ab228700)
10% SDS-PAGE
All lanes: Western blot - Anti-Arg2 antibody (ab228700) at 1/1000 dilution
Lane 1: HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract at 30 µg
Lane 2: HEK-293T cytoplasmic extract at 30 µg
Developed using the ECL technique.
Predicted band size: 39 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Arg2 antibody (ab228700)
Paraffin-embedded mouse duodenum tissue stained for Arg2 with ab228700 at 1/500 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Arg2 antibody (ab228700)
Paraffin-embedded U737 xenograft tissue stained for Arg2 with ab228700 at 1/500 dilution in immunohistochemical analysis.
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