AB228700

Anti-Arg2 antibody

Name: Anti-Arg2 antibody (ab228700) | Abcam
Brand: Abcam Limited
SKU: AB228700

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(5 Publications)

Rabbit Polyclonal Arg2 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human, Mouse samples. Cited in 5 publications. Immunogen corresponding to Recombinant Fragment Protein within Human ARG2.

View Alternative Names

Arginase II, Kidney-type arginase, Non-hepatic arginase, Type II arginase, ARG2

7 Images

Immunocytochemistry/ Immunofluorescence - Anti-Arg2 antibody (AB228700)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Arg2 antibody (AB228700)

Ice-cold methanol-fixed HepG2 (human liver hepatocellular carcinoma cell line) cells stained for Arg2 (green) using ab228700 at 1/500 dilution in ICC/IF.

Nuclear counterstain : Hoechst 33342 (blue).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Arg2 antibody (AB228700)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Arg2 antibody (AB228700)

Paraffin-embedded U737 xenograft tissue stained for Arg2 with ab228700 at 1/500 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Arg2 antibody (AB228700)

Paraffin-embedded mouse duodenum tissue stained for Arg2 with ab228700 at 1/500 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Arg2 antibody (AB228700)

Paraffin-embedded mouse kidney tissue stained for Arg2 with ab228700 at 1/500 dilution in immunohistochemical analysis.

Supplier Data

Western blot - Anti-Arg2 antibody (AB228700)

10% SDS-PAGE

All lanes:

Western blot - Anti-Arg2 antibody (ab228700) at 1/1000 dilution

Lane 1:

HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract at 30 µg

Lane 2:

HEK-293T cytoplasmic extract at 30 µg

Predicted band size: 39 kDa

true

Supplier Data

Western blot - Anti-Arg2 antibody (AB228700)

10% SDS-PAGE

All lanes:

Western blot - Anti-Arg2 antibody (ab228700) at 1/1000 dilution

Lane 1:

NT2/D2 (human carcinoma cell line from metastatic lung) whole cell lysate at 30 µg

Lane 2:

PC-3 (human prostate adenocarcinoma cell line) whole cell lysate at 30 µg

Predicted band size: 39 kDa

true

Supplier Data

Western blot - Anti-Arg2 antibody (AB228700)

Samples were separated by 10 % SDS.

All lanes:

Western blot - Anti-Arg2 antibody (ab228700) at 1/2000 dilution

Lane 1:

Non-transfected 293T whole cell extract at 50 µg

Lane 2:

Transfected 293T whole cell extract at 50 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 39 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

WB, ICC/IF, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human ARG2. The exact immunogen used to generate this antibody is proprietary information.

P78540

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "" }, "Mouse": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rhesus monkey": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: PBS, 20% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Arginase 2 (Arg2) also known as AII is an enzyme with a molecular weight of approximately 39 kDa. It is part of the urea cycle and functions to hydrolyze L-arginine into L-ornithine and urea. Arg2 is a mitochondrial enzyme mainly expressed in the liver kidney and prostate. Its expression can extend to other tissues such as the small intestine in some conditions and its activity is important in regulating arginine availability for various physiological processes.

Biological function summary

Arg2 plays a role in nitrogen disposal and polyamine synthesis. As part of an enzymatic complex it contributes to the urea cycle which is important for the removal of excess nitrogen from the body. Arginase activity also influences the balance of nitric oxide synthesis as it competes with nitric oxide synthase for their common substrate L-arginine. This competition affects cellular functions which rely on nitric oxide signaling for developing proper vascular and immune responses.

Pathways

Arg2 is an important player in the urea cycle and the polyamine synthesis pathway. It works alongside the enzymes involved in these processes such as ornithine decarboxylase. Additionally Arg2 has a role in the nitric oxide regulation pathway modulating nitric oxide levels by limiting the availability of L-arginine for nitric oxide synthase. This makes Arg2 essential for metabolic processes and cellular communication.

Altered Arg2 expression has been associated with cardiovascular diseases and renal complications. For instance disruptions in Arg2 function can lead to endothelial dysfunction influencing conditions like atherosclerosis. Arg2 imbalances are also linked to kidney diseases possibly due to its critical role in ammonia detoxification and renal arginine metabolism. Its interaction with other proteins such as nitric oxide synthase further emphasizes its relevance in these health conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

May play a role in the regulation of extra-urea cycle arginine metabolism and also in down-regulation of nitric oxide synthesis. Extrahepatic arginase functions to regulate L-arginine bioavailability to nitric oxid synthase (NOS). Arginine metabolism is a critical regulator of innate and adaptive immune responses. Seems to be involved in negative regulation of the survival capacity of activated CD4(+) and CD8(+) T cells (PubMed : 27745970). May suppress inflammation-related signaling in asthmatic airway epithelium (PubMed : 27214549). May contribute to the immune evasion of H.pylori by restricting M1 macrophage activation and polyamine metabolism (By similarity). In fetal dendritic cells may play a role in promoting immune suppression and T cell TNF production during gestation (PubMed : 28614294). Regulates RPS6KB1 signaling, which promotes endothelial cell senescence and inflammation and implicates NOS3/eNOS dysfunction (PubMed : 22928666). Can inhibit endothelial autophagy independently of its enzymatic activity implicating mTORC2 signaling (PubMed : 25484082). Involved in vascular smooth muscle cell senescence and apoptosis independently of its enzymatic activity (PubMed : 23832324). Since NOS is found in the penile corpus cavernosum smooth muscle, the clitoral corpus cavernosum and the vagina, arginase-2 plays a role in both male and female sexual arousal (PubMed : 12859189).

See full target information ARG2

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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

One health advances 3:5 PubMed40124736

2025

Arginine as host directed therapy in tuberculosis: insights from modulating arginine metabolism by supplementation and arginase inhibition.

Applications

Unspecified application

Species

Unspecified reactive species

Qingkui Jiang,Ranjeet Kumar,Yi Zhao,Selvakumar Subbian,Lanbo Shi

Cell death & disease 14:661 PubMed37816735

2023

Calbindin 2-specific deletion of arginase 2 preserves visual function after optic nerve crush.

Applications

Unspecified application

Species

Unspecified reactive species

Syed A H Zaidi,Zhimin Xu,Tahira Lemtalsi,Porsche Sandow,Sruthi Athota,Fang Liu,Stephen Haigh,Yuqing Huo,S Priya Narayanan,David J R Fulton,Modesto A Rojas,Abdelrahman Y Fouda,Robert W Caldwell,Ruth B Caldwell

Chinese medical journal 137:431-440 PubMed37690994

2023

A genetic variant in the immune-related gene ERAP1 affects colorectal cancer prognosis.

Applications

Unspecified application

Species

Unspecified reactive species

Danyi Zou,Yimin Cai,Meng Jin,Ming Zhang,Yizhuo Liu,Shuoni Chen,Shuhui Yang,Heng Zhang,Xu Zhu,Chaoqun Huang,Ying Zhu,Xiaoping Miao,Yongchang Wei,Xiaojun Yang,Jianbo Tian

Cellular and molecular life sciences : CMLS 79:41 PubMed34913090

2021

Acute hypoxia elevates arginase 2 and induces polyamine stress response in zebrafish via evolutionarily conserved mechanism.

Applications

Unspecified application

Species

Unspecified reactive species

Bodhisattwa Banerjee,Iryna Khrystoforova,Baruh Polis,Inbar Ben Zvi,David Karasik

Journal of clinical medicine 9: PubMed31979105

2020

Role of Arginase 2 in Murine Retinopathy Associated with Western Diet-Induced Obesity.

Applications

Unspecified application

Species

Unspecified reactive species

Reem T Atawia,Katharine L Bunch,Abdelrahman Y Fouda,Tahira Lemtalsi,Wael Eldahshan,Zhimin Xu,Alan Saul,Khaled Elmasry,Mohamed Al-Shabrawey,Ruth B Caldwell,R William Caldwell

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Arg2 antibody

Immunocytochemistry/ Immunofluorescence - Anti-Arg2 antibody (AB228700)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Arg2 antibody (AB228700)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Arg2 antibody (AB228700)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Arg2 antibody (AB228700)

Western blot - Anti-Arg2 antibody (AB228700)

Western blot - Anti-Arg2 antibody (AB228700)

Western blot - Anti-Arg2 antibody (AB228700)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Complementary Products

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (5)

Product promise