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AB251198

Anti-Arginine (glcnac ) antibody [EPR18251] - BSA and Azide free

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(1 Publication)

Rabbit Recombinant Monoclonal Arginine antibody. Carrier free. Suitable for WB and reacts with Modified Amino Acid samples. Cited in 1 publication.

View Alternative Names

L arginine

1 Images
Western blot - Anti-Arginine (glcnac ) antibody [EPR18251] - BSA and Azide free (AB251198)
  • WB

Supplier Data

Western blot - Anti-Arginine (glcnac ) antibody [EPR18251] - BSA and Azide free (AB251198)

This data was developed using ab195033, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

The image was kindly provided by our collaborator Dr Feng Shao's lab, NIBS.

All lanes:

Western blot - Anti-Arginine (glcnac ) antibody [EPR18251] (<a href='/en-us/products/primary-antibodies/arginine-glcnac-antibody-epr18251-ab195033'>ab195033</a>) at 1/2000 dilution

Lane 1:

HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate transfected with TRADD at 20 µg

Lane 2:

HEK-293 whole cell lysate transfected with TRADD and NleB at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR18251

Isotype

IgG

Carrier free

Yes

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Modified Amino Acid": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p>NleB contained an unprecedented N-acetylglucosamine (GlcNAc) transferase activity that specifically modified a conserved arginine in these death domains (Arg235 in the TRADD death domain).</p>" } } }
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Product details

ab251198 is the carrier-free version of ab195033.

This antibody was developed as part of a collaboration between Abcam and the lab of Dr. Feng Shao, National Institute of Biological Sciences (NIBS), Beijing.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Arginine often referred to as ARG plays a central role in various biological processes. It is an amino acid with a molecular weight of about 174.2 Da. Arginine is expressed in multiple tissues including the liver and endothelium. It often serves as a precursor for the synthesis of various biomolecules such as nitric oxide. In scientific literature you may also encounter it under alternative spellings like 'arganine' or 'argenine' though these are less common.
Biological function summary

Arginine contributes to protein synthesis and serves as a substrate for enzymes like nitric oxide synthase. It forms part of larger protein complexes and participates in metabolic pathways. Nitric oxide produced from arginine acts as a signaling molecule that modulates vascular function by inducing vasodilation. It also takes part in immune responses and has a role in cell division wound healing and hormone release.

Pathways

Arginine plays a significant role in the urea cycle and the nitric oxide signaling pathway. It interacts with proteins such as ornithine transcarbamylase in the urea cycle facilitating the removal of ammonia. Meanwhile in the nitric oxide pathway it collaborates with nitric oxide synthase to produce nitric oxide which affects smooth muscle relaxation and neurotransmission.

Arginine has connections to cardiovascular diseases and immune-associated conditions. The production of nitric oxide which involves arginine impacts cardiovascular health and its dysregulation can lead to hypertension. Additionally arginine metabolism influences conditions like arginase deficiency which affects the urea cycle. These conditions can involve other proteins such as ornithine transcarbamylase demonstrating the interlinked nature of arginine with various biological and pathological processes.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

See full target information Arginine
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

International journal of medical sciences 20:1079-1090 PubMed37484809

2023

NAT10-mediated RNA acetylation enhances HNRNPUL1 mRNA stability to contribute cervical cancer progression.

Applications

Unspecified application

Species

Unspecified reactive species

Yingfei Long,Yifei Ren,Qinglv Wei,Youchaou Mobet,Yujiao Liu,Hongyan Zhao,Tao Liu,Lei Cheng,Ping Yi
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com