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AB225937

Anti-Arginine kinase antibody

5

(1 Review)

|

(1 Publication)

Rabbit Polyclonal Arginine kinase antibody. Suitable for WB and reacts with Recombinant full length protein - Giant tiger prawn samples. Cited in 1 publication. Immunogen corresponding to Recombinant Full Length Protein corresponding to Giant tiger prawn AK.

View Alternative Names

Arginine kinase, AK

1 Images
Western blot - Anti-Arginine kinase antibody (AB225937)
  • WB

Supplier Data

Western blot - Anti-Arginine kinase antibody (AB225937)

All lanes:

Western blot - Anti-Arginine kinase antibody (ab225937) at 1/500 dilution

Lane 1:

Recombinant protein at 0.06 µg

Lane 2:

Recombinant protein at 0.03 µg

Lane 3:

Recombinant protein 15 ng at 0.015 µg

Lane 4:

Recombinant protein at 0.0075 µg

Secondary

All lanes:

Goat polyclonal to rabbit IgG at 1/50000 dilution

Predicted band size: 56 kDa

Observed band size: 56 kDa

true

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Giant tiger prawn

Applications

WB

applications

Immunogen

Recombinant Full Length Protein corresponding to Giant tiger prawn AK.

C7E3T4

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Callinectes sapidus": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Chinese mitten crab": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Common lobster": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Giant tiger prawn": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Kuruma prawn": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Marbled crab": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Recombinant full length protein - Giant tiger prawn": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/5000", "WB-species-notes": "<p></p>" }, "Shore crab": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Purification notes
Purity >95%
Storage buffer
pH: 7.4 Preservative: 0.03% Proclin 300 Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Arginine kinase also known as AK is a phosphotransferase enzyme with a molecular mass around 40 kDa. It plays an important role in cellular energy homeostasis catalyzing the reversible transfer of a phosphate group from adenosine triphosphate (ATP) to arginine forming phosphoarginine and adenosine diphosphate (ADP). This enzyme is mainly expressed in invertebrates including species like mollusks arthropods and some protozoa. Arginine kinase is not typically found in the same form in vertebrates; instead creatine kinase performs a similar function.
Biological function summary

Arginine kinase functions in a manner that ensures energy storage and transfer in cells with rapid energy demands. It operates through temporal and spatial buffering of ATP levels during high-energy usage. This makes it particularly important in facilitating activity bursts in muscle- and nerve-tissues. Arginine kinase is not commonly part of large protein complexes; it acts as a monomeric enzyme performing its role independently within the cells where it is expressed.

Pathways

This enzyme operates critically within cellular energy metabolism pathways. It connects directly with the phosphagen system an important energy reservoir for immediate cellular energy supply. Through this system arginine kinase relates closely to ATP and ADP molecules participating in the rapid regeneration of ATP from ADP. Though it is not as directly linked to glycolysis or oxidative phosphorylation as some other proteins arginine kinase supports these pathways by maintaining ATP levels constant during periods of variable energy demand.

Arginine kinase has an interesting relationship with parasitic diseases like Chagas disease caused by Trypanosoma cruzi. Some studies indicate that inhibitors targeting arginine kinase activity could provide therapeutic effects against the parasite as this enzyme appears essential for parasite survival. While it may not directly cause specific human diseases due to its absence in vertebrate physiology understanding its function and differences from human creatine kinase provides insights that are valuable for comparative enzymology and targeted drug design against invertebrate pests and parasites.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

See full target information AK
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

FASEB bioAdvances 6:53-71 PubMed38344410

2024

Club cell-specific telomere protection protein 1 (TPP1) protects against tobacco smoke-induced lung inflammation, xenobiotic metabolic dysregulation, and injurious responses.

Applications

Unspecified application

Species

Unspecified reactive species

Thivanka Muthumalage,Chiara Goracci,Irfan Rahman
View all publications
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