AB96215

Anti-Argininosuccinate Lyase antibody

Name: Anti-Argininosuccinate Lyase antibody (ab96215) | Abcam
Brand: Abcam Limited
SKU: AB96215

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(1 Publication)

Rabbit Polyclonal Argininosuccinate Lyase antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human ASL aa 50-350.

View Alternative Names

Argininosuccinate lyase, ASAL, Arginosuccinase, ASL

4 Images

Immunocytochemistry/ Immunofluorescence - Anti-Argininosuccinate Lyase antibody (AB96215)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Argininosuccinate Lyase antibody (AB96215)

HeLa cells stained for ASL (green) using ab96215 at 1/500 dilution in ICC/IF.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Argininosuccinate Lyase antibody (AB96215)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Argininosuccinate Lyase antibody (AB96215)

Paraffin embedded DLD1 xenograft stained for ASL using ab96215 at 1/500 dilution in immunohistochemical analysis.

Unknown

Western blot - Anti-Argininosuccinate Lyase antibody (AB96215)

10% SDS PAGE

All lanes:

Western blot - Anti-Argininosuccinate Lyase antibody (ab96215) at 1/1000 dilution

All lanes:

MOLT-4 whole cell lysate at 30 µg

Predicted band size: 52 kDa

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Lab

Western blot - Anti-Argininosuccinate Lyase antibody (AB96215)

All lanes:

Western blot - Anti-Argininosuccinate Lyase antibody (ab96215) at 1/1000 dilution

Lane 1:

Wild-type HeLa cell extract at 30 µg

Lane 2:

Argininosuccinate Lyase Knockout HeLa cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG

Predicted band size: 52 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IHC-P, WB, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human ASL aa 50-350. The exact immunogen used to generate this antibody is proprietary information.

P04424

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Argininosuccinate Lyase often referred to as ASL or 'lyase' is an enzyme that plays a mechanical role in the urea cycle catalyzing the conversion of argininosuccinate into arginine and fumarate. It has a molecular mass around 51 kDa. ASL is mainly expressed in the liver but is also present in the kidney and other tissues. The proper functioning of ASL is essential for maintaining the urea cycle which detoxifies ammonia in the body.

Biological function summary

This enzyme is important for the breakdown of nitrogen molecules impacting amino acid metabolism. ASL does not operate as part of a larger complex but acts independently to facilitate the removal of nitrogenous waste. Through its action it produces arginine which serves as a vital precursor for the production of nitric oxide a critical signaling molecule in many physiological processes.

Pathways

ASL is integral to the urea cycle and amino acid metabolic pathways. The urea cycle is fundamental for ammonia detoxification while amino acid metabolic pathways involve protein turnover and energy production. Within the urea cycle ASL works alongside enzymes like argininosuccinate synthetase and arginase playing a pivotal role in converting excess nitrogen to urea which the body then excretes.

Defects in ASL can lead to argininosuccinate lyase deficiency a rare disorder that causes hyperammonemia due to urea cycle dysfunction. This deficiency associates with elevated levels of argininosuccinate in the blood and urine. Additionally ASL has been implicated in vascular diseases due to its role in arginine and nitric oxide production. Its connection to nitric oxide emphasizes the potential impact on cardiovascular health with relations to proteins such as nitric oxide synthase.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

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Target data

Catalyzes the reversible cleavage of L-argininosuccinate to fumarate and L-arginine, an intermediate step reaction in the urea cycle mostly providing for hepatic nitrogen detoxification into excretable urea as well as de novo L-arginine synthesis in nonhepatic tissues (PubMed : 11747432, PubMed : 11747433, PubMed : 22081021, PubMed : 2263616, PubMed : 9045711). Essential regulator of intracellular and extracellular L-arginine pools. As part of citrulline-nitric oxide cycle, forms tissue-specific multiprotein complexes with argininosuccinate synthase ASS1, transport protein SLC7A1 and nitric oxide synthase NOS1, NOS2 or NOS3, allowing for cell-autonomous L-arginine synthesis while channeling extracellular L-arginine to nitric oxide synthesis pathway (PubMed : 22081021).

See full target information ASL

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Molecules (Basel, Switzerland) 25: PubMed31979355

2020

Dapagliflozin Inhibits Cell Adhesion to Collagen I and IV and Increases Ectodomain Proteolytic Cleavage of DDR1 by Increasing ADAM10 Activity.

Applications

Unspecified application

Species

Unspecified reactive species

Junichi Okada,Eijiro Yamada,Tsugumichi Saito,Hideaki Yokoo,Aya Osaki,Yoko Shimoda,Atsushi Ozawa,Yasuyo Nakajima,Jeffrey E Pessin,Shuichi Okada,Masanobu Yamada

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Argininosuccinate Lyase antibody

Immunocytochemistry/ Immunofluorescence - Anti-Argininosuccinate Lyase antibody (AB96215)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Argininosuccinate Lyase antibody (AB96215)

Western blot - Anti-Argininosuccinate Lyase antibody (AB96215)

Western blot - Anti-Argininosuccinate Lyase antibody (AB96215)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Complementary Products

Assay Kits

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Product promise