AB97370

Anti-Argininosuccinate Lyase antibody

Name: Anti-Argininosuccinate Lyase antibody (ab97370) | Abcam
Brand: Abcam Limited
SKU: AB97370
Rating: 4 (1 reviews)

(1 Review)

(9 Publications)

Rabbit Polyclonal Argininosuccinate Lyase antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Mouse, Human samples. Cited in 9 publications. Immunogen corresponding to Recombinant Fragment Protein within Human ASL aa 1-250.

View Alternative Names

Argininosuccinate lyase, ASAL, Arginosuccinase, ASL

6 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Argininosuccinate Lyase antibody (AB97370)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Argininosuccinate Lyase antibody (AB97370)

Paraffin embedded U-87 MG xenograft tissue stained for ASL using ab97370 at 1/500 dilution in immunohistochemical analysis.

Immunocytochemistry/ Immunofluorescence - Anti-Argininosuccinate Lyase antibody (AB97370)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Argininosuccinate Lyase antibody (AB97370)

ab97370, at a 1/200 dilution, staining Argininosuccinate Lyase in paraformaldehyde-fixed HeLa cells by Immunofluorescence analysis. Right image is merged with a DNA probe.

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Argininosuccinate Lyase antibody (AB97370)

HeLa cells stained for ASL (green) using ab97370 at 1/500 dilution in ICC/IF.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Argininosuccinate Lyase antibody (AB97370)

Paraffin embedded C2C12 xenograft tissue stained for ASL using ab97370 at 1/500 dilution in immunohistochemical analysis.

Supplier Data

Western blot - Anti-Argininosuccinate Lyase antibody (AB97370)

10% SDS-PAGE.

All lanes:

Western blot - Anti-Argininosuccinate Lyase antibody (ab97370) at 1/1000 dilution

Lane 1:

HEK-293T whole cell lysate at 30 µg

Lane 2:

A431 whole cell lysate at 30 µg

Lane 3:

HeLa whole cell lysate at 30 µg

Lane 4:

HepG2 whole cell lysate at 30 µg

Predicted band size: 52 kDa

false

Supplier Data

Western blot - Anti-Argininosuccinate Lyase antibody (AB97370)

10% SDS-PAGE.

All lanes:

Western blot - Anti-Argininosuccinate Lyase antibody (ab97370) at 1/1000 dilution

Lane 1:

Neuro2a whole cell lysate at 30 µg

Lane 2:

GL261 whole cell lysate at 30 µg

Lane 3:

C8D30 whole cell lysate at 30 µg

Lane 4:

NIH/3T3 whole cell lysate at 30 µg

Lane 5:

BCL-1 whole cell lysate at 30 µg

Lane 6:

Raw 264.7 whole cell lysate at 30 µg

Lane 7:

C2C12 whole cell lysate

Predicted band size: 52 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

WB, IHC-P, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human ASL aa 1-250. The exact immunogen used to generate this antibody is proprietary information.

P04424

style

left-column

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/200", "ICCIF-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

style

left-column

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 Preservative: 0.025% Proclin 300 Constituents: PBS, 20% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Argininosuccinate Lyase often referred to as ASL or 'lyase' is an enzyme that plays a mechanical role in the urea cycle catalyzing the conversion of argininosuccinate into arginine and fumarate. It has a molecular mass around 51 kDa. ASL is mainly expressed in the liver but is also present in the kidney and other tissues. The proper functioning of ASL is essential for maintaining the urea cycle which detoxifies ammonia in the body.

Biological function summary

This enzyme is important for the breakdown of nitrogen molecules impacting amino acid metabolism. ASL does not operate as part of a larger complex but acts independently to facilitate the removal of nitrogenous waste. Through its action it produces arginine which serves as a vital precursor for the production of nitric oxide a critical signaling molecule in many physiological processes.

Pathways

ASL is integral to the urea cycle and amino acid metabolic pathways. The urea cycle is fundamental for ammonia detoxification while amino acid metabolic pathways involve protein turnover and energy production. Within the urea cycle ASL works alongside enzymes like argininosuccinate synthetase and arginase playing a pivotal role in converting excess nitrogen to urea which the body then excretes.

Defects in ASL can lead to argininosuccinate lyase deficiency a rare disorder that causes hyperammonemia due to urea cycle dysfunction. This deficiency associates with elevated levels of argininosuccinate in the blood and urine. Additionally ASL has been implicated in vascular diseases due to its role in arginine and nitric oxide production. Its connection to nitric oxide emphasizes the potential impact on cardiovascular health with relations to proteins such as nitric oxide synthase.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Catalyzes the reversible cleavage of L-argininosuccinate to fumarate and L-arginine, an intermediate step reaction in the urea cycle mostly providing for hepatic nitrogen detoxification into excretable urea as well as de novo L-arginine synthesis in nonhepatic tissues (PubMed : 11747432, PubMed : 11747433, PubMed : 22081021, PubMed : 2263616, PubMed : 9045711). Essential regulator of intracellular and extracellular L-arginine pools. As part of citrulline-nitric oxide cycle, forms tissue-specific multiprotein complexes with argininosuccinate synthase ASS1, transport protein SLC7A1 and nitric oxide synthase NOS1, NOS2 or NOS3, allowing for cell-autonomous L-arginine synthesis while channeling extracellular L-arginine to nitric oxide synthesis pathway (PubMed : 22081021).

See full target information ASL

style

left-column

Publications (9)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 16:5677 PubMed40593809

2025

Regenerating aged bone marrow via a nitric oxide nanopump.

Applications

Unspecified application

Species

Unspecified reactive species

Ke Li,Sihan Hu,Hanwen Li,Wenzheng Lin,Duoyi Zhao,Zhuobin Xu,Chun Pan,Huihui Wang,Dandan Li,Jingjing Liu,Hao Chen

Aging cell 22:e13954 PubMed37614052

2023

Alterations of lipid-mediated mitophagy result in aging-dependent sensorimotor defects.

Applications

Unspecified application

Species

Unspecified reactive species

Natalia Oleinik,Onder Albayram,Mohamed Faisal Kassir,F Cansu Atilgan,Chase Walton,Eda Karakaya,John Kurtz,Alexander Alekseyenko,Habeeb Alsudani,Megan Sheridan,Zdzislaw M Szulc,Besim Ogretmen

Human genetics 140:1471-1485 PubMed34417872

2021

ASL expression in ALDH1A1 neurons in the substantia nigra metabolically contributes to neurodegenerative phenotype.

Applications

Unspecified application

Species

Unspecified reactive species

Shaul Lerner,Raya Eilam,Lital Adler,Julien Baruteau,Topaz Kreiser,Michael Tsoory,Alexander Brandis,Tevie Mehlman,Mina Ryten,Juan A Botia,Sonia Garcia Ruiz,Alejandro Cisterna Garcia,Carlo Dionisi-Vici,Giusy Ranucci,Marco Spada,Ram Mazkereth,Robert McCarter,Rima Izem,Thomas J Balmat,Rachel Richesson,Ehud Gazit,Sandesh C S Nagamani,Ayelet Erez

Biomedical chromatography : BMC 35:e5020 PubMed33159321

2021

The related mechanism of complete Freund's adjuvant-induced chronic inflammation pain based on metabolomics analysis.

Applications

Unspecified application

Species

Unspecified reactive species

Weibo Zhang,Jie Lyu,Juxiang Xu,Piao Zhang,Shuxia Zhang,Yeru Chen,Yongjie Wang,Gang Chen

Cell 182:1606-1622.e23 PubMed32888429

2020

The Human and Mouse Enteric Nervous System at Single-Cell Resolution.

Applications

Unspecified application

Species

Unspecified reactive species

Eugene Drokhlyansky,Christopher S Smillie,Nicholas Van Wittenberghe,Maria Ericsson,Gabriel K Griffin,Gokcen Eraslan,Danielle Dionne,Michael S Cuoco,Max N Goder-Reiser,Tatyana Sharova,Olena Kuksenko,Andrew J Aguirre,Genevieve M Boland,Daniel Graham,Orit Rozenblatt-Rosen,Ramnik J Xavier,Aviv Regev

Cell reports 29:2144-2153.e7 PubMed31747589

2019

ASL Metabolically Regulates Tyrosine Hydroxylase in the Nucleus Locus Coeruleus.

Applications

Unspecified application

Species

Unspecified reactive species

Shaul Lerner,Elmira Anderzhanova,Sima Verbitsky,Raya Eilam,Yael Kuperman,Michael Tsoory,Yuri Kuznetsov,Alexander Brandis,Tevie Mehlman,Ram Mazkereth,Robert McCarter,Menahem Segal,Sandesh C S Nagamani,Alon Chen,Ayelet Erez

Nature communications 9:3505 PubMed30158522

2018

Argininosuccinic aciduria fosters neuronal nitrosative stress reversed by Asl gene transfer.

Applications

Unspecified application

Species

Unspecified reactive species

Julien Baruteau,Dany P Perocheau,Joanna Hanley,Maëlle Lorvellec,Eridan Rocha-Ferreira,Rajvinder Karda,Joanne Ng,Natalie Suff,Juan Antinao Diaz,Ahad A Rahim,Michael P Hughes,Blerida Banushi,Helen Prunty,Mariya Hristova,Deborah A Ridout,Alex Virasami,Simon Heales,Stewen J Howe,Suzanne M K Buckley,Philippa B Mills,Paul Gissen,Simon N Waddington

Cell 174:1559-1570.e22 PubMed30100185

2018

Urea Cycle Dysregulation Generates Clinically Relevant Genomic and Biochemical Signatures.

Applications

Unspecified application

Species

Unspecified reactive species

Joo Sang Lee,Lital Adler,Hiren Karathia,Narin Carmel,Shiran Rabinovich,Noam Auslander,Rom Keshet,Noa Stettner,Alon Silberman,Lilach Agemy,Daniel Helbling,Raya Eilam,Qin Sun,Alexander Brandis,Sergey Malitsky,Maxim Itkin,Hila Weiss,Sivan Pinto,Shelly Kalaora,Ronen Levy,Eilon Barnea,Arie Admon,David Dimmock,Noam Stern-Ginossar,Avigdor Scherz,Sandesh C S Nagamani,Miguel Unda,David M Wilson,Ronit Elhasid,Arkaitz Carracedo,Yardena Samuels,Sridhar Hannenhalli,Eytan Ruppin,Ayelet Erez

American journal of physiology. Endocrinology and 313:E233-E242 PubMed28611027

2017

The intestinal-renal axis for arginine synthesis is present and functional in the neonatal pig.

Applications

Unspecified application

Species

Unspecified reactive species

Juan C Marini,Umang Agarwal,Jason L Robinson,Yang Yuan,Inka C Didelija,Barbara Stoll,Douglas G Burrin

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Argininosuccinate Lyase antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Argininosuccinate Lyase antibody (AB97370)

Immunocytochemistry/ Immunofluorescence - Anti-Argininosuccinate Lyase antibody (AB97370)

Immunocytochemistry/ Immunofluorescence - Anti-Argininosuccinate Lyase antibody (AB97370)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Argininosuccinate Lyase antibody (AB97370)

Western blot - Anti-Argininosuccinate Lyase antibody (AB97370)

Western blot - Anti-Argininosuccinate Lyase antibody (AB97370)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Complementary Products

Primary Antibodies

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (9)

Product promise