Anti-Argininosuccinate Lyase antibody [EPR19382]

• IP

Supplier Data

Immunoprecipitation - Anti-Argininosuccinate Lyase antibody [EPR19382] (AB201025)

Argininosuccinate Lyase was immunoprecipitated from 1mg of Human fetal liver whole cell lysate with ab201025 at 1/40 dilution.

Western blot was performed from the immunoprecipitate using ab201025 at 1/1000 dilution.

VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.

Lane 1 : Human fetal liver whole cell lysate, 10μg (Input).

Lane 2 : ab201025 IP in Human fetal liver whole cell lysate.

Lane 3 : Rabbit IgG,monoclonal-Isotype Control (ab172730) instead of ab201025 in Human fetal liver whole cell lysate.

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 5 seconds.

All lanes:

Immunoprecipitation - Anti-Argininosuccinate Lyase antibody [EPR19382] (ab201025)

Predicted band size: 52 kDa

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• WB

Supplier Data

Western blot - Anti-Argininosuccinate Lyase antibody [EPR19382] (AB201025)

Blocking/Dilution buffer : 5% NFDM/TBST.

Exposure time : Lane 1,2 and 3 : 8 seconds; Lane 4 : 1 second.

All lanes:

Western blot - Anti-Argininosuccinate Lyase antibody [EPR19382] (ab201025) at 1/1000 dilution

Lane 1:

Human fetal heart tissue lysate at 10 µg

Lane 2:

Human fetal kidney tissue lysate at 10 µg

Lane 3:

Human fetal spleen tissue lysate at 10 µg

Lane 4:

Human fetal liver tissue lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/100000 dilution

Predicted band size: 52 kDa

Observed band size: 51 kDa

false

• WB

Supplier Data

Western blot - Anti-Argininosuccinate Lyase antibody [EPR19382] (AB201025)

Blocking/Dilution buffer : 5% NFDM/TBST.

Exposure time : Lane 1 and 2 : 1 second; Lane 3,4 and 5 : 15 seconds; Lane 6 and 7 : 30 seconds.

Dexamethasone treatment increase the accumulation of ASL mRNA (PMID : 2209616).

All lanes:

Western blot - Anti-Argininosuccinate Lyase antibody [EPR19382] (ab201025) at 1/1000 dilution

Lane 1:

Untreated HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate at 20 µg

Lane 2:

HepG2 (Human liver hepatocellular carcinoma cell line) treated with 1 μM dexamethasone for 24 hours whole cell lysate at 20 µg

Lane 3:

HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate at 20 µg

Lane 4:

HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 20 µg

Lane 5:

T-47D (Human ductal breast epithelial tumor cell line) whole cell lysate at 20 µg

Lane 6:

A549 (Human lung carcinoma cell line) whole cell lysate at 20 µg

Lane 7:

SH-SY5Y (Human neuroblastoma cell line from bone marrow) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 52 kDa

Observed band size: 51 kDa

false