Rabbit Recombinant Monoclonal Argininosuccinate Lyase antibody. Suitable for IP, WB and reacts with Human, Mouse, Rat samples. Cited in 3 publications.
Images
![Western blot - Anti-Argininosuccinate Lyase antibody [EPR19396] (AB201026), expandable thumbnail](https://content.abcam.com/products/images/argininosuccinate-lyase-antibody-epr19396-ab201026--western-blot-img10808.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Argininosuccinate Lyase antibody [EPR19396] (AB201026)")
![Western blot - Anti-Argininosuccinate Lyase antibody [EPR19396] (AB201026), expandable thumbnail](https://content.abcam.com/products/images/argininosuccinate-lyase-antibody-epr19396-ab201026--western-blot-img137986.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Argininosuccinate Lyase antibody [EPR19396] (AB201026)")
![Western blot - Anti-Argininosuccinate Lyase antibody [EPR19396] (AB201026), expandable thumbnail](https://content.abcam.com/products/images/argininosuccinate-lyase-antibody-epr19396-ab201026--western-blot-img107411.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Argininosuccinate Lyase antibody [EPR19396] (AB201026)")
![Immunoprecipitation - Anti-Argininosuccinate Lyase antibody [EPR19396] (AB201026), expandable thumbnail](https://content.abcam.com/products/images/argininosuccinate-lyase-antibody-epr19396-ab201026--immunoprecipitation-img10782.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-Argininosuccinate Lyase antibody [EPR19396] (AB201026)")
![Western blot - Anti-Argininosuccinate Lyase antibody [EPR19396] (AB201026), expandable thumbnail](https://content.abcam.com/products/images/argininosuccinate-lyase-antibody-epr19396-ab201026--western-blot-img21103.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Argininosuccinate Lyase antibody [EPR19396] (AB201026)")
Publications
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- EMBO molecular medicine 13:e131582020Beclin-1-mediated activation of autophagy improves proximal and distal urea cycle disorders.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLeandro R Soria,Sonam Gurung,Giulia De Sabbata,Dany P Perocheau,Angela De Angelis,Gemma Bruno,Elena Polishchuk,Debora Paris,Paola Cuomo,Andrea Motta,Michael Orford,Youssef Khalil,Simon Eaton,Philippa B Mills,Simon N Waddington,Carmine Settembre,Andrés F Muro,Julien Baruteau,Nicola Brunetti-PierriPubMed 33369168
- PloS one 15:e02316332020A modified arginine-depleting enzyme NEI-01 inhibits growth of pancreatic cancer cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJeremy P H Chow et. al.PubMed 32353864
- Cell metabolism 30:352-363.e82019Polyamines and eIF5A Hypusination Modulate Mitochondrial Respiration and Macrophage Activation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDaniel J Puleston et. al.PubMed 31130465
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IP | WB | |
|---|---|---|
| Human | Tested | Tested |
| Mouse | Expected | Tested |
| Rat | Expected | Tested |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/30 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/2000 | Notes - |
Species Rat | Dilution info 1/2000 | Notes - |
Species Human | Dilution info 1/2000 | Notes - |
Associated Products
Select an associated product type
3 products for Alternative Product
Target data
Function
Catalyzes the reversible cleavage of L-argininosuccinate to fumarate and L-arginine, an intermediate step reaction in the urea cycle mostly providing for hepatic nitrogen detoxification into excretable urea as well as de novo L-arginine synthesis in nonhepatic tissues (PubMed:11747432, PubMed:11747433, PubMed:22081021, PubMed:2263616, PubMed:9045711). Essential regulator of intracellular and extracellular L-arginine pools. As part of citrulline-nitric oxide cycle, forms tissue-specific multiprotein complexes with argininosuccinate synthase ASS1, transport protein SLC7A1 and nitric oxide synthase NOS1, NOS2 or NOS3, allowing for cell-autonomous L-arginine synthesis while channeling extracellular L-arginine to nitric oxide synthesis pathway (PubMed:22081021).
Alternative names
Argininosuccinate lyase, ASAL, Arginosuccinase, ASL
Recommended products
Rabbit Recombinant Monoclonal Argininosuccinate Lyase antibody. Suitable for IP, WB and reacts with Human, Mouse, Rat samples. Cited in 3 publications.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EPR19396
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Argininosuccinate Lyase often referred to as ASL or 'lyase' is an enzyme that plays a mechanical role in the urea cycle catalyzing the conversion of argininosuccinate into arginine and fumarate. It has a molecular mass around 51 kDa. ASL is mainly expressed in the liver but is also present in the kidney and other tissues. The proper functioning of ASL is essential for maintaining the urea cycle which detoxifies ammonia in the body.
- Biological function summary
This enzyme is important for the breakdown of nitrogen molecules impacting amino acid metabolism. ASL does not operate as part of a larger complex but acts independently to facilitate the removal of nitrogenous waste. Through its action it produces arginine which serves as a vital precursor for the production of nitric oxide a critical signaling molecule in many physiological processes.
- Pathways
ASL is integral to the urea cycle and amino acid metabolic pathways. The urea cycle is fundamental for ammonia detoxification while amino acid metabolic pathways involve protein turnover and energy production. Within the urea cycle ASL works alongside enzymes like argininosuccinate synthetase and arginase playing a pivotal role in converting excess nitrogen to urea which the body then excretes.
- Associated diseases and disorders
Defects in ASL can lead to argininosuccinate lyase deficiency a rare disorder that causes hyperammonemia due to urea cycle dysfunction. This deficiency associates with elevated levels of argininosuccinate in the blood and urine. Additionally ASL has been implicated in vascular diseases due to its role in arginine and nitric oxide production. Its connection to nitric oxide emphasizes the potential impact on cardiovascular health with relations to proteins such as nitric oxide synthase.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
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5 product images
![Western blot - Anti-Argininosuccinate Lyase antibody [EPR19396] (ab201026), expandable thumbnail](https://content.abcam.com/products/images/argininosuccinate-lyase-antibody-epr19396-ab201026--western-blot-img10808.jpg "Western blot - Anti-Argininosuccinate Lyase antibody [EPR19396] (ab201026)")
Western blot - Anti-Argininosuccinate Lyase antibody [EPR19396] (ab201026)
Blocking/Dilution buffer: 5% NFDM/TBST.
Exposure time: Lane 1: 2 seconds; Lane 2: 1 minute; Lane 3 and 4: 5 seconds; Lane 5 and 6: 1 second.
All lanes: Western blot - Anti-Argininosuccinate Lyase antibody [EPR19396] (ab201026) at 1/5000 dilution
Lane 1: Human fetal liver tissue lysate at 20 µg
Lane 2: Human testis tissue lysate at 20 µg
Lane 3: HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate at 20 µg
Lane 4: T-47D (Human ductal breast epithelial tumor cell line) whole cell lysate at 20 µg
Lane 5: Mouse liver lysate at 20 µg
Lane 6: Rat liver lysate at 20 µg
SecondaryAll lanes: Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution
Predicted band size: 52 kDa
Observed band size: 51 kDa
![Western blot - Anti-Argininosuccinate Lyase antibody [EPR19396] (ab201026), expandable thumbnail](https://content.abcam.com/products/images/argininosuccinate-lyase-antibody-epr19396-ab201026--western-blot-img137986.jpg "Western blot - Anti-Argininosuccinate Lyase antibody [EPR19396] (ab201026)")
Western blot - Anti-Argininosuccinate Lyase antibody [EPR19396] (ab201026)
Blocking/Dilution buffer: 5% NFDM/TBST.
All lanes: Western blot - Anti-Argininosuccinate Lyase antibody [EPR19396] (ab201026) at 1/2000 dilution
Lane 1: A549 (Human lung carcinoma cell line) whole cell lysate at 20 µg
Lane 2: SH-SY5Y (Human neuroblastoma cell line from bone marrow) whole cell lysate at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Predicted band size: 52 kDa
Observed band size: 51 kDa
Exposure time: 1min
![Western blot - Anti-Argininosuccinate Lyase antibody [EPR19396] (ab201026), expandable thumbnail](https://content.abcam.com/products/images/argininosuccinate-lyase-antibody-epr19396-ab201026--western-blot-img107411.jpg "Western blot - Anti-Argininosuccinate Lyase antibody [EPR19396] (ab201026)")
Western blot - Anti-Argininosuccinate Lyase antibody [EPR19396] (ab201026)
Blocking/Dilution buffer: 5% NFDM/TBST.
All lanes: Western blot - Anti-Argininosuccinate Lyase antibody [EPR19396] (ab201026) at 1/2000 dilution
Lane 1: C6 (Rat glial tumor cell line) whole cell lysate at 20 µg
Lane 2: RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate at 20 µg
Lane 3: PC-12 (Rat adrenal gland pheochromocytoma cell line) whole cell lysate at 20 µg
Lane 4: NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Predicted band size: 52 kDa
Observed band size: 51 kDa
Exposure time: 15s
![Immunoprecipitation - Anti-Argininosuccinate Lyase antibody [EPR19396] (ab201026), expandable thumbnail](https://content.abcam.com/products/images/argininosuccinate-lyase-antibody-epr19396-ab201026--immunoprecipitation-img10782.jpg "Immunoprecipitation - Anti-Argininosuccinate Lyase antibody [EPR19396] (ab201026)")
Immunoprecipitation - Anti-Argininosuccinate Lyase antibody [EPR19396] (ab201026)
Argininosuccinate Lyase was immunoprecipitated from 0.35 mg of Human fetal liver whole cell lysate with ab201026 at 1/30 dilution.
Western blot was performed from the immunoprecipitate using ab201026 at 1/1000 dilution.
VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366), was used for detection at 1/10000 dilution.
Lane 1: Human fetal liver whole cell lysate, 10μg (Input).
Lane 2: ab201026 IP in Human fetal liver whole cell lysate.
Lane 3: Rabbit IgG,monoclonal [EPR25A]-Isotype Control (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab201026 in Human fetal liver whole cell lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 1 second.
All lanes: Immunoprecipitation - Anti-Argininosuccinate Lyase antibody [EPR19396] (ab201026)
Predicted band size: 52 kDa
![Western blot - Anti-Argininosuccinate Lyase antibody [EPR19396] (ab201026), expandable thumbnail](https://content.abcam.com/products/images/argininosuccinate-lyase-antibody-epr19396-ab201026--western-blot-img21103.jpg "Western blot - Anti-Argininosuccinate Lyase antibody [EPR19396] (ab201026)")
Western blot - Anti-Argininosuccinate Lyase antibody [EPR19396] (ab201026)
Blocking/Dilution buffer: 5% NFDM/TBST.
Argininosuccinate Lyase is predominantly expressed in the liver, and weakly expressed in the lung (PMID: 8576237).
All lanes: Western blot - Anti-Argininosuccinate Lyase antibody [EPR19396] (ab201026) at 1/5000 dilution
Lane 1: Human fetal liver lysate at 20 µg
Lane 2: Human fetal lung lysate at 20 µg
SecondaryAll lanes: Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution
Predicted band size: 105 kDa, 41 kDa, 49 kDa, 52 kDa
Observed band size: 105 kDa, 41 kDa, 51 kDa, 55-60 kDa
Exposure time: 2s
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