Anti-Argininosuccinate Lyase antibody [EPR19396] - BSA and Azide free

• IP

Supplier Data

Immunoprecipitation - Anti-Argininosuccinate Lyase antibody [EPR19396] - BSA and Azide free (AB251331)

This data was developed using ab201026, the same antibody clone in a different buffer formulation.

Argininosuccinate Lyase was immunoprecipitated from 0.35 mg of Human fetal liver whole cell lysate with ab201026 at 1/30 dilution.

Western blot was performed from the immunoprecipitate using ab201026 at 1/1000 dilution.

VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.

Lane 1 : Human fetal liver whole cell lysate, 10μg (Input).
Lane 2 : ab201026 IP in Human fetal liver whole cell lysate.
Lane 3 : Rabbit IgG,monoclonal [EPR25A]-Isotype Control (ab172730) instead of ab201026 in Human fetal liver whole cell lysate.

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 1 second.

All lanes:

Immunoprecipitation - Anti-Argininosuccinate Lyase antibody [EPR19396] (<a href='/en-us/products/primary-antibodies/argininosuccinate-lyase-antibody-epr19396-ab201026'>ab201026</a>)

Predicted band size: 52 kDa

false

• WB

Supplier Data

Western blot - Anti-Argininosuccinate Lyase antibody [EPR19396] - BSA and Azide free (AB251331)

This data was developed using ab201026, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

Argininosuccinate Lyase is predominantly expressed in the liver, and weakly expressed in the lung (PMID : 8576237).

All lanes:

Western blot - Anti-Argininosuccinate Lyase antibody [EPR19396] (<a href='/en-us/products/primary-antibodies/argininosuccinate-lyase-antibody-epr19396-ab201026'>ab201026</a>) at 1/5000 dilution

Lane 1:

Human fetal liver lysate at 20 µg

Lane 2:

Human fetal lung lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution

Predicted band size: 105 kDa,41 kDa,49 kDa,52 kDa

Observed band size: 105 kDa,41 kDa,51 kDa,55-60 kDa

false

Exposure time: 2s

• WB

Supplier Data

Western blot - Anti-Argininosuccinate Lyase antibody [EPR19396] - BSA and Azide free (AB251331)

This data was developed using ab201026, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

Exposure times : Lane 1 : 2 seconds; Lane 2 : 1 minute; Lanes 3-4 : 5 seconds; Lanes 5-6 : 1 second.

All lanes:

Western blot - Anti-Argininosuccinate Lyase antibody [EPR19396] (<a href='/en-us/products/primary-antibodies/argininosuccinate-lyase-antibody-epr19396-ab201026'>ab201026</a>) at 1/5000 dilution

Lane 1:

Human fetal liver tissue lysate at 20 µg

Lane 2:

Human testis tissue lysate at 20 µg

Lane 3:

HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate at 20 µg

Lane 4:

T-47D (Human ductal breast epithelial tumor cell line) whole cell lysate at 20 µg

Lane 5:

Mouse liver lysate at 20 µg

Lane 6:

Rat liver lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution

Predicted band size: 52 kDa

Observed band size: 51 kDa

false

• WB

Supplier Data

Western blot - Anti-Argininosuccinate Lyase antibody [EPR19396] - BSA and Azide free (AB251331)

This data was developed using ab201026, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-Argininosuccinate Lyase antibody [EPR19396] (<a href='/en-us/products/primary-antibodies/argininosuccinate-lyase-antibody-epr19396-ab201026'>ab201026</a>) at 1/2000 dilution

Lane 1:

A549 (Human lung carcinoma cell line) whole cell lysate at 20 µg

Lane 2:

SH-SY5Y (Human neuroblastoma cell line from bone marrow) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 52 kDa

Observed band size: 51 kDa

false

Exposure time: 1min

• WB

Supplier Data

Western blot - Anti-Argininosuccinate Lyase antibody [EPR19396] - BSA and Azide free (AB251331)

This data was developed using ab201026, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-Argininosuccinate Lyase antibody [EPR19396] (<a href='/en-us/products/primary-antibodies/argininosuccinate-lyase-antibody-epr19396-ab201026'>ab201026</a>) at 1/2000 dilution

Lane 1:

C6 (Rat glial tumor cell line) whole cell lysate at 20 µg

Lane 2:

RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate at 20 µg

Lane 3:

PC-12 (Rat adrenal gland pheochromocytoma cell line) whole cell lysate at 20 µg

Lane 4:

NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 52 kDa

Observed band size: 51 kDa

false

Exposure time: 15s