anti-Argonaute-2 antibody [2E12-1C9] ab57113 is a carrier free mouse monoclonal antibody that is used in Argonaute-2 western blotting, IHC, immunofluorescence and flow cytometry. Suitable for human samples.
- PBS only, conjugation-ready, removing anything extra from your antibodies, greater flexibility in assay design
- One antibody for all your Argonaute-2 staining
- Antibody clone 2E12-1C9 has been tried and trusted by researchers since 2007
Images
![Flow Cytometry - Anti-Argonaute-2 antibody [2E12-1C9] - BSA and Azide free (AB57113), expandable thumbnail](https://content.abcam.com/products/images/argonaute-2-antibody-2e12-1c9-bsa-and-azide-free-ab57113--flow-cytometry-img125484.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry - Anti-Argonaute-2 antibody [2E12-1C9] - BSA and Azide free (AB57113)")
![Western blot - Anti-Argonaute-2 antibody [2E12-1C9] - BSA and Azide free (AB57113), expandable thumbnail](https://content.abcam.com/products/images/argonaute-2-antibody-2e12-1c9-bsa-and-azide-free-ab57113--western-blot-img64474.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Argonaute-2 antibody [2E12-1C9] - BSA and Azide free (AB57113)")
![Immunocytochemistry/ Immunofluorescence - Anti-Argonaute-2 antibody [2E12-1C9] - BSA and Azide free (AB57113), expandable thumbnail](https://content.abcam.com/products/images/argonaute-2-antibody-2e12-1c9-bsa-and-azide-free-ab57113--immunocytochemistry-immunofluorescence-img133206.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-Argonaute-2 antibody [2E12-1C9] - BSA and Azide free (AB57113)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Argonaute-2 antibody [2E12-1C9] - BSA and Azide free (AB57113), expandable thumbnail](https://content.abcam.com/products/images/argonaute-2-antibody-2e12-1c9-bsa-and-azide-free-ab57113--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img16198.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Argonaute-2 antibody [2E12-1C9] - BSA and Azide free (AB57113)")
![Western blot - Anti-Argonaute-2 antibody [2E12-1C9] - BSA and Azide free (AB57113), expandable thumbnail](https://content.abcam.com/products/images/argonaute-2-antibody-2e12-1c9-bsa-and-azide-free-ab57113--western-blot-img35339.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Argonaute-2 antibody [2E12-1C9] - BSA and Azide free (AB57113)")
Publications
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- Nature biotechnology :2023Detection of transcriptome-wide microRNA-target interactions in single cells with agoTRIBE.Applications:Unspecified applicationReactive species:Unspecified reactive speciesVaishnovi Sekar,Emilio Mármol-Sánchez,Panagiotis Kalogeropoulos,Laura Stanicek,Eduardo A Sagredo,Albin Widmark,Evangelos Doukoumopoulos,Franziska Bonath,Inna Biryukova,Marc R FriedländerPubMed 37735263
- iScience 26:1078372023Nuclear miR-150 enhances hepatic lipid accumulation by targeting RNA transcripts overlapping the promoter.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJiao Luo,Yanan Ji,Ningning Chen,Ge Song,Shuyue Zhou,Xuan Niu,Dianke YuPubMed 37736048
- Communications biology 6:8852023Selective enrichment of plasma cell-free messenger RNA in cancer-associated extracellular vesicles.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHyun Ji Kim,Matthew J Rames,Florian Goncalves,C Ward Kirschbaum,Breeshey Roskams-Hieter,Elias Spiliotopoulos,Josephine Briand,Aaron Doe,Joseph Estabrook,Josiah T Wagner,Emek Demir,Gordon Mills,Thuy T M NgoPubMed 37644220
- Cells 12:2023Nucleolin Regulates Pulmonary Artery Smooth Muscle Cell Proliferation under Hypoxia by Modulating miRNA Expression.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJihui Lee,Hara KangPubMed 36899956
- The Journal of biological chemistry 299:1045772023Serine 970 of RNA helicase MOV10 is phosphorylated and controls unfolding activity and fate of mRNAs targeted for AGO2-mediated silencing.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAatiqa Nawaz,Phillip J Kenny,Temirlan Shilikbay,Matt Reed,Olga Stuchlik,Jan Pohl,Stephanie CemanPubMed 36871759
- Science advances 9:eadf05972023The Integrator complex regulates microRNA abundance through RISC loading.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNina Kirstein,Sadat Dokaneheifard,Pradeep Reddy Cingaram,Monica Guiselle Valencia,Felipe Beckedorff,Helena Gomes Dos Santos,Ezra Blumenthal,Mina Masoumeh Tayari,Gabriel Stephen Gaidosh,Ramin ShiekhattarPubMed 36763664
- Molecular cancer 22:162023A novel polypeptide encoded by the circular RNA ZKSCAN1 suppresses HCC via degradation of mTOR.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRunjie Song,Shuoqian Ma,Jiajia Xu,Xin Ren,Peilan Guo,Huijiao Liu,Peng Li,Fan Yin,Mei Liu,Qiang Wang,Lei Yu,Jiali Liu,Binwei Duan,Nafis A Rahman,Sławomir Wołczyński,Guangming Li,Xiangdong LiPubMed 36691031
- FASEB journal : official publication of the Federation of American Societies for Experimental Biology 37:e226572022Long noncoding RNA UCA1 promotes the expression and function of P-glycoprotein by sponging miR-16-5p in human placental BeWo cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChangqing Tang,Yuxin Deng,Shuran Shao,Yafei Guo,Lixia Yang,Yu Yan,Yi Zhang,Dajian Qiu,Kaiyu Zhou,Yimin Hua,Chuan WangPubMed 36459147
- Journal of translational medicine 20:5472022Circular RNA circLOC101928570 suppresses systemic lupus erythematosus progression by targeting the miR-150-5p/c-myb axis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXingwang Zhao,Rui Dong,Zhongwei Tang,Juan Wang,Chunyou Wang,Zhiqiang Song,Bing Ni,Longlong Zhang,Xiaochong He,Yi YouPubMed 36435781
- Nature communications 13:38252022Single-molecule FRET uncovers hidden conformations and dynamics of human Argonaute 2.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSarah Willkomm,Leonhard Jakob,Kevin Kramm,Veronika Graus,Julia Neumeier,Gunter Meister,Dina GrohmannPubMed 35780145
Key facts
- Isotype
- IgG1
- Host species
- Mouse
- Storage buffer
pH: 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- Recombinant Fragment Protein within Human AGO2 aa 450 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q9UKV8
Reactivity data
Select an application| Flow Cyt | WB | IHC-P | ICC/IF | |
|---|---|---|---|---|
| Human | Tested | Tested | Tested | Tested |
| Xenopus laevis | Not recommended | Not recommended | Not recommended | Not recommended |
Flow Cyt
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Xenopus laevis | Dilution info - | Notes ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Xenopus laevis | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Xenopus laevis | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Xenopus laevis | Dilution info - | Notes - |
Associated Products
Select an associated product type
6 products for Alternative Product
Target data
Function
Required for RNA-mediated gene silencing (RNAi) by the RNA-induced silencing complex (RISC). The 'minimal RISC' appears to include AGO2 bound to a short guide RNA such as a microRNA (miRNA) or short interfering RNA (siRNA). These guide RNAs direct RISC to complementary mRNAs that are targets for RISC-mediated gene silencing. The precise mechanism of gene silencing depends on the degree of complementarity between the miRNA or siRNA and its target. Binding of RISC to a perfectly complementary mRNA generally results in silencing due to endonucleolytic cleavage of the mRNA specifically by AGO2. Binding of RISC to a partially complementary mRNA results in silencing through inhibition of translation, and this is independent of endonuclease activity. May inhibit translation initiation by binding to the 7-methylguanosine cap, thereby preventing the recruitment of the translation initiation factor eIF4-E. May also inhibit translation initiation via interaction with EIF6, which itself binds to the 60S ribosomal subunit and prevents its association with the 40S ribosomal subunit. The inhibition of translational initiation leads to the accumulation of the affected mRNA in cytoplasmic processing bodies (P-bodies), where mRNA degradation may subsequently occur. In some cases RISC-mediated translational repression is also observed for miRNAs that perfectly match the 3' untranslated region (3'-UTR). Can also up-regulate the translation of specific mRNAs under certain growth conditions. Binds to the AU element of the 3'-UTR of the TNF (TNF-alpha) mRNA and up-regulates translation under conditions of serum starvation. Also required for transcriptional gene silencing (TGS), in which short RNAs known as antigene RNAs or agRNAs direct the transcriptional repression of complementary promoter regions. (Microbial infection) Upon Sars-CoV-2 infection, associates with viral miRNA-like small RNA, CoV2-miR-O7a, and may repress mRNAs, such as BATF2, to evade the IFN response.
Alternative names
EIF2C2, AGO2, Protein argonaute-2, Argonaute2, hAgo2, Argonaute RISC catalytic component 2, Eukaryotic translation initiation factor 2C 2, PAZ Piwi domain protein, Protein slicer, eIF-2C 2, eIF2C 2, PPD
Recommended products
anti-Argonaute-2 antibody [2E12-1C9] ab57113 is a carrier free mouse monoclonal antibody that is used in Argonaute-2 western blotting, IHC, immunofluorescence and flow cytometry. Suitable for human samples.
- PBS only, conjugation-ready, removing anything extra from your antibodies, greater flexibility in assay design
- One antibody for all your Argonaute-2 staining
- Antibody clone 2E12-1C9 has been tried and trusted by researchers since 2007
Key facts
- Isotype
- IgG1
- Host species
- Mouse
- Storage buffer
pH: 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- Recombinant Fragment Protein within Human AGO2 aa 450 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q9UKV8
- Carrier free
- Yes
- Clone number
- 2E12-1C9
- Purification technique
- Affinity purification Protein A
- Light chain type
- kappa
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
This product was changed from ascites to tissue culture supernatant on 30th April 2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
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Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Argonaute-2 often referred to as Ago2 or Argonaute-2 protein plays an important role in the RNA-induced silencing complex (RISC). It has a molecular weight of approximately 97 kDa. Ago2 is found in the cytoplasm and nucleus of cells and is expressed in a wide range of tissues including the brain and liver. Its mechanical function involves binding to guide RNA and facilitating strand cleavage which is essential for gene silencing and regulation at the post-transcriptional level.
- Biological function summary
The Argonaute-2 protein participates in gene silencing mechanisms. As a part of RISC Ago2 binds to microRNAs (miRNAs) or small interfering RNAs (siRNAs) to direct sequence-specific cleavage of target mRNAs regulating their translation and stability. This protein is a part of a multi-protein complex that modulates gene expression and influences cellular homeostasis and development. In its biological role Argonaute-2 impacts cellular differentiation proliferation and apoptosis.
- Pathways
Several cellular processes require Argonaute-2 notably the RNA interference (RNAi) and microRNA (miRNA) pathways. In these pathways it interacts with Dicer a protein essential for the maturation of small RNA molecules and facilitates the gene silencing process. Ago2 is also involved in the regulation of gene expression during embryonic development and stress responses indicating its importance in maintaining cellular function and responding to environmental changes.
- Associated diseases and disorders
Anomalies in Argonaute-2 activity correlate with cancer including lung and breast cancer where dysregulation of miRNA pathways can lead to unchecked cellular proliferation. It is also linked to neurological disorders like schizophrenia possibly due to its role in gene expression regulation in neural cells. Interactions between Ago2 and proteins like Dicer and other Argonautes suggest complex regulatory networks that when disrupted contribute to these diseases.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
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6 product images
![Flow Cytometry - Anti-Argonaute-2 antibody [2E12-1C9] - BSA and Azide free (ab57113), expandable thumbnail](https://content.abcam.com/products/images/argonaute-2-antibody-2e12-1c9-bsa-and-azide-free-ab57113--flow-cytometry-img125484.jpg "Flow Cytometry - Anti-Argonaute-2 antibody [2E12-1C9] - BSA and Azide free (ab57113)")
Flow Cytometry - Anti-Argonaute-2 antibody [2E12-1C9] - BSA and Azide free (ab57113)
Overlay histogram showing HeLa cells stained with ab57113 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab57113, 1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (Goat Anti-Mouse IgG H&L (DyLight® 488) preadsorbed ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (Mouse IgG1, Kappa Monoclonal [B11/6] - Isotype Control ab91353, 2μg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde/permeabilized in 0.1% PBS-Tween used under the same conditions.
This image was generated using the ascites version of the product.
![Western blot - Anti-Argonaute-2 antibody [2E12-1C9] - BSA and Azide free (ab57113), expandable thumbnail](https://content.abcam.com/products/images/argonaute-2-antibody-2e12-1c9-bsa-and-azide-free-ab57113--western-blot-img64474.jpg "Western blot - Anti-Argonaute-2 antibody [2E12-1C9] - BSA and Azide free (ab57113)")
Western blot - Anti-Argonaute-2 antibody [2E12-1C9] - BSA and Azide free (ab57113)
All lanes: Western blot - Anti-Argonaute-2 antibody [2E12-1C9] - BSA and Azide free (ab57113)
All lanes: MCF7 cell lysate
Predicted band size: 97 kDa
Observed band size: 97 kDa
![Immunocytochemistry/ Immunofluorescence - Anti-Argonaute-2 antibody [2E12-1C9] - BSA and Azide free (ab57113), expandable thumbnail](https://content.abcam.com/products/images/argonaute-2-antibody-2e12-1c9-bsa-and-azide-free-ab57113--immunocytochemistry-immunofluorescence-img133206.jpg "Immunocytochemistry/ Immunofluorescence - Anti-Argonaute-2 antibody [2E12-1C9] - BSA and Azide free (ab57113)")
Immunocytochemistry/ Immunofluorescence - Anti-Argonaute-2 antibody [2E12-1C9] - BSA and Azide free (ab57113)
Ago2 / eIF2C2 antibody (ab57113) used in immunofluorescence at 10ug/ml on HeLa cells.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Argonaute-2 antibody [2E12-1C9] - BSA and Azide free (ab57113), expandable thumbnail](https://content.abcam.com/products/images/argonaute-2-antibody-2e12-1c9-bsa-and-azide-free-ab57113--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img16198.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Argonaute-2 antibody [2E12-1C9] - BSA and Azide free (ab57113)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Argonaute-2 antibody [2E12-1C9] - BSA and Azide free (ab57113)
Ago2 / eIF2C2 antibody (ab57113) used in immunohistochemistry at 3ug/ml on formalin fixed and paraffin embedded human stomach.
![Western blot - Anti-Argonaute-2 antibody [2E12-1C9] - BSA and Azide free (ab57113), expandable thumbnail](https://content.abcam.com/products/images/argonaute-2-antibody-2e12-1c9-bsa-and-azide-free-ab57113--western-blot-img35339.jpg "Western blot - Anti-Argonaute-2 antibody [2E12-1C9] - BSA and Azide free (ab57113)")
Western blot - Anti-Argonaute-2 antibody [2E12-1C9] - BSA and Azide free (ab57113)
The band at about 42 kDa corresponds to the recombinant fragment of human Argonaute-2 aa 483-859 (377 aa length).
The loading control is GAPDH.
This image was generated using the ascites version of the product.
All lanes: Western blot - Anti-Argonaute-2 antibody [2E12-1C9] - BSA and Azide free (ab57113) at 5 µg/mL
Lane 1: Argonaute-2 overexpressing HEK-293 cells line
Lane 2: Argonaute-2 overexpressing HEK-293 cell line cotransfected with Argonaute-2 validated chimera RNAi.
Predicted band size: 97 kDa
Observed band size: 42 kDa
![Western blot - Anti-Argonaute-2 antibody [2E12-1C9] - BSA and Azide free (ab57113), expandable thumbnail](https://content.abcam.com/products/images/argonaute-2-antibody-2e12-1c9-bsa-and-azide-free-ab57113--western-blot-img36446.jpg "Western blot - Anti-Argonaute-2 antibody [2E12-1C9] - BSA and Azide free (ab57113)")
Western blot - Anti-Argonaute-2 antibody [2E12-1C9] - BSA and Azide free (ab57113)
Western Blot detection against the recombinant fragment imunogen (68 KDa for a.a. ~377 plus GST tag +26 kDa).
This image was generated using the ascites version of the product.
All lanes: Western blot - Anti-Argonaute-2 antibody [2E12-1C9] - BSA and Azide free (ab57113)
Predicted band size: 97 kDa
Observed band size: 68 kDa
![Western blot - Anti-Argonaute-2 antibody [2E12-1C9] - BSA and Azide free (ab57113), expandable thumbnail](https://content.abcam.com/products/images/argonaute-2-antibody-2e12-1c9-bsa-and-azide-free-ab57113--western-blot-img36446.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Argonaute-2 antibody [2E12-1C9] - BSA and Azide free (ab57113)")
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