AB57113

Anti-Argonaute-2 antibody [2E12-1C9] - BSA and Azide free

Name: Anti-Argonaute-2 antibody [2E12-1C9] - Carrier free (ab57113) | Abcam
Brand: Abcam Limited
SKU: AB57113
Price: 425 USD
Availability: InStock
Rating: 4 (10 reviews)

(10 Reviews)

(181 Publications)

Anti-Argonaute-2 antibody [2E12-1C9] - BSA and Azide free (ab57113) is a mouse monoclonal antibody provided in a PBS only buffer for easy conjugation detecting Argonaute-2 in Western Blot, Flow Cytometry, IHC-P, ICC/IF. Suitable for Human,.

- BSA, sodium azide, and glycerol-free for easy conjugation
- Over 160 publications
- Trusted since 2007

View Alternative Names

EIF2C2, AGO2, Protein argonaute-2, Argonaute2, hAgo2, Argonaute RISC catalytic component 2, Eukaryotic translation initiation factor 2C 2, PAZ Piwi domain protein, Protein slicer, eIF-2C 2, eIF2C 2, PPD

6 Images

Supplier Data

Western blot - Anti-Argonaute-2 antibody [2E12-1C9] - BSA and Azide free (AB57113)

The band at about 42 kDa corresponds to the recombinant fragment of human Argonaute-2 aa 483-859 (377 aa length).

The loading control is GAPDH.

This image was generated using the ascites version of the product.

All lanes:

Western blot - Anti-Argonaute-2 antibody [2E12-1C9] - BSA and Azide free (ab57113) at 5 µg/mL

Lane 1:

Argonaute-2 overexpressing HEK-293 cells line

Lane 2:

Argonaute-2 overexpressing HEK-293 cell line cotransfected with Argonaute-2 validated chimera RNAi.

Predicted band size: 97 kDa

Observed band size: 42 kDa

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Immunocytochemistry/ Immunofluorescence - Anti-Argonaute-2 antibody [2E12-1C9] - BSA and Azide free (AB57113)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Argonaute-2 antibody [2E12-1C9] - BSA and Azide free (AB57113)

Ago2 / eIF2C2 antibody (ab57113) used in immunofluorescence at 10ug/ml on HeLa cells.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Argonaute-2 antibody [2E12-1C9] - BSA and Azide free (AB57113)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Argonaute-2 antibody [2E12-1C9] - BSA and Azide free (AB57113)

Ago2 / eIF2C2 antibody (ab57113) used in immunohistochemistry at 3ug/ml on formalin fixed and paraffin embedded human stomach.

Flow Cytometry - Anti-Argonaute-2 antibody [2E12-1C9] - BSA and Azide free (AB57113)

Flow Cyt

Unknown

Flow Cytometry - Anti-Argonaute-2 antibody [2E12-1C9] - BSA and Azide free (AB57113)

Overlay histogram showing HeLa cells stained with ab57113 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab57113, 1μg/1x10⁶ cells) for 30 min at 22°C. The secondary antibody used was DyLight^® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2μg/1x10⁶ cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde/permeabilized in 0.1% PBS-Tween used under the same conditions.

This image was generated using the ascites version of the product.

Unknown

Western blot - Anti-Argonaute-2 antibody [2E12-1C9] - BSA and Azide free (AB57113)

Western Blot detection against the recombinant fragment imunogen (68 KDa for a.a. ~377 plus GST tag +26 kDa).

This image was generated using the ascites version of the product.

All lanes:

Western blot - Anti-Argonaute-2 antibody [2E12-1C9] - BSA and Azide free (ab57113)

Predicted band size: 97 kDa

Observed band size: 68 kDa

false

Supplier Data

Western blot - Anti-Argonaute-2 antibody [2E12-1C9] - BSA and Azide free (AB57113)

All lanes:

Western blot - Anti-Argonaute-2 antibody [2E12-1C9] - BSA and Azide free (ab57113)

All lanes:

MCF7 cell lysate

Predicted band size: 97 kDa

Observed band size: 97 kDa

false

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

2E12-1C9

Isotype

IgG1

Light chain type

kappa

Carrier free

Yes

Reacts with

Human

Applications

WB, Flow Cyt, ICC/IF, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human AGO2 aa 450 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q9UKV8

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<a href='/en-us/products/primary-antibodies/mouse-igg1-kappa-monoclonal-15-6e10a7-isotype-control-ab170190'>ab170190</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Xenopus laevis": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<a href='/en-us/products/primary-antibodies/mouse-igg1-kappa-monoclonal-15-6e10a7-isotype-control-ab170190'>ab170190</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Product details

What is this antibody validated in?
Anti-Argonaute-2 antibody [2E12-1C9] - BSA and Azide free (ab57113) is a mouse monoclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Flow Cyt), Immunohistochemistry (IHC-P), Immunocytochemistry/immunofluorescence (ICC/IF) in Human, samples.

What is the molecular weight of Argonaute-2?
Anti-Argonaute-2 [2E12-1C9] - BSA and Azide free (ab57113) specifically detects a band for Argonaute-2 (UniProt: Q9UKV8) at a molecular weight of 97kDa.

Trusted by the scientific community
Anti-Argonaute-2 [2E12-1C9] - BSA and Azide free (ab57113) was first used in a scientific publication in 2007 and has been cited over 160 times in peer-reviewed journals.

Reviewed by scientists
Anti-Argonaute-2 [2E12-1C9] - BSA and Azide free (ab57113) has over 5 independent reviews from customers.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Argonaute-2 often referred to as Ago2 or Argonaute-2 protein plays an important role in the RNA-induced silencing complex (RISC). It has a molecular weight of approximately 97 kDa. Ago2 is found in the cytoplasm and nucleus of cells and is expressed in a wide range of tissues including the brain and liver. Its mechanical function involves binding to guide RNA and facilitating strand cleavage which is essential for gene silencing and regulation at the post-transcriptional level.

Biological function summary

The Argonaute-2 protein participates in gene silencing mechanisms. As a part of RISC Ago2 binds to microRNAs (miRNAs) or small interfering RNAs (siRNAs) to direct sequence-specific cleavage of target mRNAs regulating their translation and stability. This protein is a part of a multi-protein complex that modulates gene expression and influences cellular homeostasis and development. In its biological role Argonaute-2 impacts cellular differentiation proliferation and apoptosis.

Pathways

Several cellular processes require Argonaute-2 notably the RNA interference (RNAi) and microRNA (miRNA) pathways. In these pathways it interacts with Dicer a protein essential for the maturation of small RNA molecules and facilitates the gene silencing process. Ago2 is also involved in the regulation of gene expression during embryonic development and stress responses indicating its importance in maintaining cellular function and responding to environmental changes.

Anomalies in Argonaute-2 activity correlate with cancer including lung and breast cancer where dysregulation of miRNA pathways can lead to unchecked cellular proliferation. It is also linked to neurological disorders like schizophrenia possibly due to its role in gene expression regulation in neural cells. Interactions between Ago2 and proteins like Dicer and other Argonautes suggest complex regulatory networks that when disrupted contribute to these diseases.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Required for RNA-mediated gene silencing (RNAi) by the RNA-induced silencing complex (RISC). The 'minimal RISC' appears to include AGO2 bound to a short guide RNA such as a microRNA (miRNA) or short interfering RNA (siRNA). These guide RNAs direct RISC to complementary mRNAs that are targets for RISC-mediated gene silencing. The precise mechanism of gene silencing depends on the degree of complementarity between the miRNA or siRNA and its target. Binding of RISC to a perfectly complementary mRNA generally results in silencing due to endonucleolytic cleavage of the mRNA specifically by AGO2. Binding of RISC to a partially complementary mRNA results in silencing through inhibition of translation, and this is independent of endonuclease activity. May inhibit translation initiation by binding to the 7-methylguanosine cap, thereby preventing the recruitment of the translation initiation factor eIF4-E. May also inhibit translation initiation via interaction with EIF6, which itself binds to the 60S ribosomal subunit and prevents its association with the 40S ribosomal subunit. The inhibition of translational initiation leads to the accumulation of the affected mRNA in cytoplasmic processing bodies (P-bodies), where mRNA degradation may subsequently occur. In some cases RISC-mediated translational repression is also observed for miRNAs that perfectly match the 3' untranslated region (3'-UTR). Can also up-regulate the translation of specific mRNAs under certain growth conditions. Binds to the AU element of the 3'-UTR of the TNF (TNF-alpha) mRNA and up-regulates translation under conditions of serum starvation. Also required for transcriptional gene silencing (TGS), in which short RNAs known as antigene RNAs or agRNAs direct the transcriptional repression of complementary promoter regions.. (Microbial infection) Upon Sars-CoV-2 infection, associates with viral miRNA-like small RNA, CoV2-miR-O7a, and may repress mRNAs, such as BATF2, to evade the IFN response.

See full target information AGO2

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Publications (181)

Recent publications for all applications. Explore the full list and refine your search

Journal of orthopaedic surgery and research 20:749 PubMed40781640

2025

Circular RNA GCLC promotes osteogenic differentiation of bone marrow mesenchymal stem cells via modulating miR-516b-5p/GNAS axis.

Applications

Unspecified application

Species

Unspecified reactive species

An Li,Sheng Zhang,Jing Zhang,Zhihua Peng,Qinghui Feng

Molecular medicine reports 32: PubMed40641140

2025

Centrosome‑, mitotic spindle‑ and cytokinetic bridge‑specific compartmentalization of AGO2 protein in human liver cells undergoing mitosis: Non‑canonical, RNAi‑dependent, control of local homeostasis.

Applications

Unspecified application

Species

Unspecified reactive species

Eleni I Theotoki,Panos Kakoulidis,Konstantinos-Stylianos Nikolakopoulos,Eleni N Vlachou,Ourania E Tsitsilonis,Gerassimos E Voutsinas,Ema Anastasiadou,Dimitrios J Stravopodis

RNA biology 22:1-17 PubMed40590376

2025

SARS-CoV-2 RNA-binding protein suppresses extracellular miRNA release.

Applications

Unspecified application

Species

Unspecified reactive species

Hyejin Mun,Chang Hoon Shin,Qingxuan Fei,Andrea Estefania Lopez Giraldo,Kyoung-Min Choi,Ji Won Lee,Kyungmin Kim,Kyung-Won Min,Leilei Shi,Mark T Bedford,Dong-Chan Kim,Yoo Lim Chun,Seonghyun Ryu,Dongin Kim,Jeong Ho Chang,Ryan T Westrope,Michelle Shay,Edward Nguyen,Junho K Hur,Abigail Agyenda,Nam Chul Kim,Sung-Ung Kang,Woonghee Lee,Je-Hyun Yoon

Frontiers in pharmacology 16:1592846 PubMed40313617

2025

LINC01559 drives osimertinib resistance in NSCLC through a ceRNA network regulating miR-320a/IGF2BP3 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Leidi Xu,Yibo Zhang,Liangliang Xing,Ying Zhou,Ning Chang,Hangtian Xi,Xiangrui Xu,Jian Zhang

Molecular therapy. Nucleic acids 36:102516 PubMed40242045

2025

Mechanistic intracellular PK/PD modeling to inform development strategies for small interfering RNA therapeutics.

Applications

Unspecified application

Species

Unspecified reactive species

Lin Chen,Caroline Bosmajian,Sukyung Woo

Cell reports. Medicine 6:101997 PubMed40054457

2025

siRNA-AGO2 complex inhibits bacterial gene translation: A promising therapeutic strategy for superbug infection.

Applications

Unspecified application

Species

Unspecified reactive species

Chen Wang,Wangjian Sheng,Yu Zhou,Xudong Hang,Jiayi Zhao,Yuanyuan Gu,Xiangfeng Meng,Yuefan Bai,Weili Li,Yujing Zhang,Linlin Zhang,Jing Yu,Zhen Zhou,Xiaona Li,Haorui Sun,Yanhong Xue,Tao Xu,Ke Zen,Hong Ling,Chen-Yu Zhang,Hongkai Bi,Huan Wang

iScience 27:110904 PubMed39398251

2024

Possible involvement of a MEG3-miR-21-SPRY1-NF-κB feedback loop in spermatogenic cells proliferation, autophagy, and apoptosis.

Applications

Unspecified application

Species

Unspecified reactive species

Xingyu Fang,Xiaotong Lu,Yujie Ma,Ning Sun,Yunyun Jiao,Hui Meng,Mengjiao Song,Haixia Jin,Guidong Yao,Ning Song,Zhaoting Wu,Shuang Wen,Haoran Guo,Haosen Xiong,Wenyan Song

Molecular therapy. Nucleic acids 35:102335 PubMed39380712

2024

regulates extrahepatic siRNA-mediated gene silencing efficacy.

Applications

Unspecified application

Species

Unspecified reactive species

Jiamiao Lu,Jasper Lee,Eric Yuan,Devin L Wakefield,Matt Kanke,Danielle Pruitt,Jose Barreda,Ingrid C Rulifson,Jiansong Xie,John Ferbas,Jason Long,Bryan Meade,Oliver Homann,Wei Guo,Tina Gomes,Hong Zhou,Bin Wu,Jixin Cui,Songli Wang

Journal of cell science 137: PubMed39308343

2024

Mature microRNA-binding protein QKI suppresses extracellular microRNA let-7b release.

Applications

Unspecified application

Species

Unspecified reactive species

Kyung-Won Min,Kyoung-Min Choi,Hyejin Mun,Seungbeom Ko,Ji Won Lee,Cari A Sagum,Mark T Bedford,Young-Kook Kim,Joe R Delaney,Jung-Hyun Cho,Ted M Dawson,Valina L Dawson,Waleed Twal,Dong-Chan Kim,Clarisse H Panganiban,Hainan Lang,Xin Zhou,Seula Shin,Jian Hu,Tilman Heise,Sang-Ho Kwon,Dongsan Kim,Young Hwa Kim,Sung-Ung Kang,Kyungmin Kim,Sydney Lewis,Ahmet Eroglu,Seonghyun Ryu,Dongin Kim,Jeong Ho Chang,Junyang Jung,Je-Hyun Yoon

BMC cancer 24:1126 PubMed39256686

2024

GLIDR-mediated regulation of tumor malignancy and cisplatin resistance in non-small cell lung cancer via the miR-342-5p/PPARGC1A axis.

Applications

Unspecified application

Species

Unspecified reactive species

Ruihua Liu,Jiemin Wang,Lichun Zhang,Shu Wang,Xiangnan Li,Yueshi Liu,Haiquan Yu

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Argonaute-2 antibody [2E12-1C9] - BSA and Azide free

Western blot - Anti-Argonaute-2 antibody [2E12-1C9] - BSA and Azide free (AB57113)

Immunocytochemistry/ Immunofluorescence - Anti-Argonaute-2 antibody [2E12-1C9] - BSA and Azide free (AB57113)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Argonaute-2 antibody [2E12-1C9] - BSA and Azide free (AB57113)

Flow Cytometry - Anti-Argonaute-2 antibody [2E12-1C9] - BSA and Azide free (AB57113)

Western blot - Anti-Argonaute-2 antibody [2E12-1C9] - BSA and Azide free (AB57113)

Western blot - Anti-Argonaute-2 antibody [2E12-1C9] - BSA and Azide free (AB57113)

Key facts

Host species

Clonality

Clone number

Isotype

Light chain type

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (181)

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