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Rabbit Polyclonal Argonaute-2 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human, Rat samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human AGO2.

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Images

Western blot - Anti-Argonaute-2 antibody (AB226943), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Argonaute-2 antibody (AB226943), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-Argonaute-2 antibody (AB226943), expandable thumbnail

Publications

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine)

Form

Liquid

Clonality

Polyclonal

Immunogen

  • Synthetic Peptide within Human AGO2. The exact immunogen used to generate this antibody is proprietary information. Database link Q9UKV8

Consider this alternative

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBIHC-PICC/IF
Human
Tested
Expected
Tested
Mouse
Predicted
Predicted
Predicted
Rat
Expected
Tested
Expected
Cow
Predicted
Predicted
Predicted
Pig
Predicted
Predicted
Predicted
Rabbit
Predicted
Predicted
Predicted

Tested
Tested

Species

Human

Dilution info

1/500 - 1/3000

Notes

-

Expected
Expected

Species

Rat

Dilution info

Use at an assay dependent concentration.

Notes

-

Predicted
Predicted

Species

Mouse, Rabbit, Cow, Pig

Dilution info

-

Notes

-

Tested
Tested

Species

Rat

Dilution info

1/100 - 1/1000

Notes

-

Expected
Expected

Species

Human

Dilution info

Use at an assay dependent concentration.

Notes

-

Predicted
Predicted

Species

Mouse, Rabbit, Cow, Pig

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

1/100 - 1/1000

Notes

-

Expected
Expected

Species

Rat

Dilution info

Use at an assay dependent concentration.

Notes

-

Predicted
Predicted

Species

Mouse, Rabbit, Cow, Pig

Dilution info

-

Notes

-

Associated Products

Select an associated product type

5 products for Alternative Product

Target data

Function

Required for RNA-mediated gene silencing (RNAi) by the RNA-induced silencing complex (RISC). The 'minimal RISC' appears to include AGO2 bound to a short guide RNA such as a microRNA (miRNA) or short interfering RNA (siRNA). These guide RNAs direct RISC to complementary mRNAs that are targets for RISC-mediated gene silencing. The precise mechanism of gene silencing depends on the degree of complementarity between the miRNA or siRNA and its target. Binding of RISC to a perfectly complementary mRNA generally results in silencing due to endonucleolytic cleavage of the mRNA specifically by AGO2. Binding of RISC to a partially complementary mRNA results in silencing through inhibition of translation, and this is independent of endonuclease activity. May inhibit translation initiation by binding to the 7-methylguanosine cap, thereby preventing the recruitment of the translation initiation factor eIF4-E. May also inhibit translation initiation via interaction with EIF6, which itself binds to the 60S ribosomal subunit and prevents its association with the 40S ribosomal subunit. The inhibition of translational initiation leads to the accumulation of the affected mRNA in cytoplasmic processing bodies (P-bodies), where mRNA degradation may subsequently occur. In some cases RISC-mediated translational repression is also observed for miRNAs that perfectly match the 3' untranslated region (3'-UTR). Can also up-regulate the translation of specific mRNAs under certain growth conditions. Binds to the AU element of the 3'-UTR of the TNF (TNF-alpha) mRNA and up-regulates translation under conditions of serum starvation. Also required for transcriptional gene silencing (TGS), in which short RNAs known as antigene RNAs or agRNAs direct the transcriptional repression of complementary promoter regions.(Microbial infection) Upon Sars-CoV-2 infection, associates with viral miRNA-like small RNA, CoV2-miR-O7a, and may repress mRNAs, such as BATF2, to evade the IFN response.

Alternative names

Recommended products

Rabbit Polyclonal Argonaute-2 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human, Rat samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human AGO2.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Polyclonal

Immunogen
  • Synthetic Peptide within Human AGO2. The exact immunogen used to generate this antibody is proprietary information. Database link Q9UKV8
Purification technique

Affinity purification Immunogen

Concentration
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Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

Argonaute-2 often referred to as Ago2 or Argonaute-2 protein plays an important role in the RNA-induced silencing complex (RISC). It has a molecular weight of approximately 97 kDa. Ago2 is found in the cytoplasm and nucleus of cells and is expressed in a wide range of tissues including the brain and liver. Its mechanical function involves binding to guide RNA and facilitating strand cleavage which is essential for gene silencing and regulation at the post-transcriptional level.

Biological function summary

The Argonaute-2 protein participates in gene silencing mechanisms. As a part of RISC Ago2 binds to microRNAs (miRNAs) or small interfering RNAs (siRNAs) to direct sequence-specific cleavage of target mRNAs regulating their translation and stability. This protein is a part of a multi-protein complex that modulates gene expression and influences cellular homeostasis and development. In its biological role Argonaute-2 impacts cellular differentiation proliferation and apoptosis.

Pathways

Several cellular processes require Argonaute-2 notably the RNA interference (RNAi) and microRNA (miRNA) pathways. In these pathways it interacts with Dicer a protein essential for the maturation of small RNA molecules and facilitates the gene silencing process. Ago2 is also involved in the regulation of gene expression during embryonic development and stress responses indicating its importance in maintaining cellular function and responding to environmental changes.

Associated diseases and disorders

Anomalies in Argonaute-2 activity correlate with cancer including lung and breast cancer where dysregulation of miRNA pathways can lead to unchecked cellular proliferation. It is also linked to neurological disorders like schizophrenia possibly due to its role in gene expression regulation in neural cells. Interactions between Ago2 and proteins like Dicer and other Argonautes suggest complex regulatory networks that when disrupted contribute to these diseases.

Product promise

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3 product images

  • Western blot - Anti-Argonaute-2 antibody (ab226943), expandable thumbnail

    Western blot - Anti-Argonaute-2 antibody (ab226943)

    All lanes: Western blot - Anti-Argonaute-2 antibody (ab226943) at 1/1000 dilution

    Lane 1: MCF7 (human breast adenocarcinoma cell line) whole cell extract at 30 µg

    Lane 2: MDA-MB-231 (human breast adenocarcinoma cell line) whole cell extract at 30 µg

    Developed using the ECL technique.

    Predicted band size: 97 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Argonaute-2 antibody (ab226943), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Argonaute-2 antibody (ab226943)

    Paraffin-embedded rat liver tissue stained for Argonaute-2 with ab226943 at 1/500 dilution in immunohistochemical analysis.

  • Immunocytochemistry/ Immunofluorescence - Anti-Argonaute-2 antibody (ab226943), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-Argonaute-2 antibody (ab226943)

    MCF7 (human breast adenocarcinoma cell line) cells stained for Argonaute-2 (green) using ab226943 at 1/500 dilution in ICC/IF.

    Cells were fixed in 4% paraformaldehyde for 15 minutes at RT. Blue: Hoechst 33342 staining.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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