Anti-Argonaute-2 antibody

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Argonaute-2 antibody (AB32381)

IHC image of Argonaute-2 antibody staining in a section of formalin-fixed paraffin-embedded human breast adenocarcinoma* performed on a Leica BOND^TM system using the standard protocol. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab32381, 5ug/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

• IHC-P

PubMed

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Argonaute-2 antibody (AB32381)

Immunohistochemical analysis of Argonaute2 (AGO2) in human tissues sections from slowly progressive, rapidly progressive, or normal biopsies.

Representative images of slowly progressive (A–B, E–F), rapidly progressive (C–D, G–H), and normal (I–J) biopsies stained with IgG control (A, C, E, G, & I) and anti-AGO2 antibody (B, D, F, H, & J) are shown. Images A, B, C, & D were stained with antibody concentrations of 20 μg/ml and images E, F, G, H, I, & J were stained with an antibody concentration of 4 μg/ml. Sections were counterstained with hematoxylin. Protein expression stains brown in this procedure (original magnification : ×200).

Panels A-D shown.

Oak et al PLoS One. 2011;6(6):e21253. doi: 10.1371/journal.pone.0021253. Epub 2011 Jun 21. Fig 5. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/

• WB

Project

Western blot - Anti-Argonaute-2 antibody (AB32381)

The identification of the 55 kDa band is unclear but this band has also been observed in HeLa lysates in the Western Blot of ab5072, targeting Drosophila Ago2 / eIF2C2.

All lanes:

Western blot - Anti-Argonaute-2 antibody (ab32381) at 2 µg/mL

Lane 1:

HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 20 µg

Lane 2:

Western blot - Jurkat whole cell lysate (<a href='/en-us/products/cell-lysates/jurkat-whole-cell-lysate-ab7899'>ab7899</a>) at 20 µg

Lane 3:

HeLa (Human epithelial carcinoma cell line) Nuclear Lysate at 20 µg

Secondary

All lanes:

IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution

Predicted band size: 97 kDa

Observed band size: 55 kDa,87 kDa

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