Anti-Argonaute-2 antibody [EPR10410] - BSA and Azide free

• Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-Argonaute-2 antibody [EPR10410] - BSA and Azide free (AB249284)

This data was developed using ab156870, the same antibody clone in a different buffer formulation.

Intracellular Flow Cytometry analysis of HeLa (human cervix adenocarcinoma) cells labeling Argonaute-2 (red) with unpurified ab156870 at a 1/200 dilution. Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. A goat anti-rabbit IgG (Alexa Fluor^® 488) (ab150077) was used as the secondary antibody at a 1/2000 dilution. Black - Rabbit monoclonal IgG (ab172730). Blue (unlabeled control) - Cells without incubation with the primary and secondary antibodies.

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Argonaute-2 antibody [EPR10410] - BSA and Azide free (AB249284)

This data was developed using ab156870, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue labeling Ago2 / eIF2C2 with unpurified ab156870 at 1/50 dilution.

Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.

• ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-Argonaute-2 antibody [EPR10410] - BSA and Azide free (AB249284)

This data was developed using ab156870, the same antibody clone in a different buffer formulation.

Immunocytochemistry/ Immunofluorescence analysis of MCF-7 (Human breast adenocarcinoma epithelial cell) cells labeling Argonaute-2 with Purified ab156870 at 1 : 200 dilution (9.5μg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) 1 : 200 (2.5 μg/ml). ab150077 Goat anti rabbit IgG(Alexa Fluor^® 488) was used as the secondary antibody at 1 : 1000 dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Argonaute-2 antibody [EPR10410] - BSA and Azide free (AB249284)

This data was developed using ab156870, the same antibody clone in a different buffer formulation.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human ovarian carcinoma tissue sections labeling Argonaute -2 with purified ab156870 at 1 : 100 dilution (1.9 μg/ml). Heat mediated antigen retrieval was performed using EDTA Buffer, pH9.0. Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) secondary antibody was used at 1 : 0 dilution. PBS instead of the primary antibody was used as the negative control.

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Argonaute-2 antibody [EPR10410] - BSA and Azide free (AB249284)

This data was developed using ab156870, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling Ago2 / eIF2C2 with unpurified ab156870 at 1/50 dilution.

Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.

• IP

Lab

Immunoprecipitation - Anti-Argonaute-2 antibody [EPR10410] - BSA and Azide free (AB249284)

This data was developed using ab156870, the same antibody clone in a different buffer formulation. Argonaute-2 was immunoprecipitated from HeLa cell lysate with ab156870 at 1 : 30 dilution (2µg in 0.35mg lysates). Western blot was performed from the immunoprecipitate using ab156870 at 1/1000 dilution. Secondary antibody VeriBlot for IP secondary antibody (HRP) (ab131366) was used at 1/5000 dilution.  Lane 1 : HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate  Lane 2 : HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab156870 in HeLa whole cell lysate  Blocking and diluting buffer : 5% NFDM/TBST

All lanes:

Immunoprecipitation - Anti-Argonaute-2 antibody [EPR10410] (<a href='/en-us/products/primary-antibodies/argonaute-2-antibody-epr10410-ab156870'>ab156870</a>)

Lanes 1 - 2:

HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate

Lane 3:

Rabbit monoclonal IgG (<a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a>) instead of <a href='/en-us/products/primary-antibodies/argonaute-2-antibody-epr10410-ab156870'>ab156870</a> in HeLa whole cell lysate

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (<a href='/en-us/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/5000 dilution

false

• WB

Unknown

Western blot - Anti-Argonaute-2 antibody [EPR10410] - BSA and Azide free (AB249284)

This data was developed using ab156870, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-Argonaute-2 antibody [EPR10410] (<a href='/en-us/products/primary-antibodies/argonaute-2-antibody-epr10410-ab156870'>ab156870</a>) at 1/1000 dilution

Lane 1:

HeLa cell lysate at 10 µg

Lane 2:

MCF7 cell lysate at 10 µg

Lane 3:

HepG2 cell lysate at 10 µg

Lane 4:

K562 cell lysate at 10 µg

Secondary

All lanes:

Goat anti-rabbit HRP at 1/2000 dilution

Predicted band size: 97 kDa

false

• WB

Lab

Western blot - Anti-Argonaute-2 antibody [EPR10410] - BSA and Azide free (AB249284)

This data was developed using ab156870, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-Argonaute-2 antibody [EPR10410] (<a href='/en-us/products/primary-antibodies/argonaute-2-antibody-epr10410-ab156870'>ab156870</a>) at 1/1000 dilution

Lane 1:

HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates at 15 µg

Lane 2:

HUVEC (Human umbilical vein endothelial cell) whole cell lysates at 15 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 97 kDa

false

• WB

Lab

Western blot - Anti-Argonaute-2 antibody [EPR10410] - BSA and Azide free (AB249284)

This data was developed using ab156870, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-Argonaute-2 antibody [EPR10410] (<a href='/en-us/products/primary-antibodies/argonaute-2-antibody-epr10410-ab156870'>ab156870</a>) at 1/5000 dilution

Lane 1:

Rat liver lysates at 20 µg

Lane 2:

Mouse kidney lysates at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 97 kDa

false