Anti-Argonaute-2 antibody [EPR10411]
- KO Validated
- RabMAb
- Recombinant
- What is this?
Be the first to review this product! Submit a review
|
(393 Publications)
Anti-Argonaute-2 antibody [EPR10411] (ab186733) is a rabbit monoclonal antibody detecting Argonaute-2 in Western Blot, Flow Cytometry (Intra), IP, IHC-P, ICC/IF. Suitable for Human, Mouse, Rat.
- KO validated for confirmed specificity
- Biophysical QC for unrivalled batch-batch consistency
- Over 230 publications
View Alternative Names
EIF2C2, AGO2, Protein argonaute-2, Argonaute2, hAgo2, Argonaute RISC catalytic component 2, Eukaryotic translation initiation factor 2C 2, PAZ Piwi domain protein, Protein slicer, eIF-2C 2, eIF2C 2, PPD
- IHC-P
Supplier Data
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Argonaute-2 antibody [EPR10411] (AB186733)
Immunohistochemical analysis of paraffin-embedded Human cervix carcinoma tissue labeling Ago2 / eIF2C2 with ab186733 at 1/100 dilution followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.
Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
- ICC/IF
Supplier Data
Immunocytochemistry/ Immunofluorescence - Anti-Argonaute-2 antibody [EPR10411] (AB186733)
Immunofluorescent analysis of 4% paraformaldehyde-fixed HeLa cells labeling Ago2 / eIF2C2 with ab186733 at 1/250 dilution followed by Goat anti rabbit IgG (Alexa Fluor® 488) secondary antibody at 1/200 dilution. Counter stained with Dapi.
- IHC-P
Lab
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Argonaute-2 antibody [EPR10411] (AB186733)
Immunohistochemical analysis of formalin fixed paraffin embedded human colon AC labelling Argonaute-2 with ab186733 at a concentration of 0.5 μg/ml. The immunostaining was performed on a Ventana DISCOVERY ULTRA (Roche Tissue Diagnostics) instrument with a OptiView DAB IHC Detection Kit. The heat-mediated antigen retrieval was conducted with at 100 °C, pH 8.5 for 32 mins. ab186733 Anti-Argonaute-2 antibody [EPR10411] was incubated for 16 mins, at 37 °C. Sections were counterstained with Hematoxylin II. Image inset shows absence of staining in secondary antibody only control. Customers are encouraged to optimise antigen retrieval conditions, antibody concentration, incubation times and temperature for best results in their own IHC assay workflow (automated and manual).
- Flow Cyt (Intra)
Supplier Data
Flow Cytometry (Intracellular) - Anti-Argonaute-2 antibody [EPR10411] (AB186733)
Intracellular flow cytometric analysis of2% paraformaldehyde-fixed MCF7 cells labeling Ago2 / eIF2C2 with ab186733 at 1/60 dilution (red) compared to aRabbit monoclonal IgG isotype control (green), followed byGoat anti rabbit IgG (FITC) secondary antibody at 1/150 dilution.
- IP
Supplier Data
Immunoprecipitation - Anti-Argonaute-2 antibody [EPR10411] (AB186733)
Western blot analysis of Ago2 / eIF2C2 in MCF7 cell lysate immunoprecipitated with ab186733 at 1/50 dilution.
Secondary antibody : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution.
All lanes:
Immunoprecipitation - Anti-Argonaute-2 antibody [EPR10411] (ab186733)
Predicted band size: 97 kDa
false
- IHC-P
Supplier Data
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Argonaute-2 antibody [EPR10411] (AB186733)
Immunohistochemical analysis of paraffin-embedded Mouse kidney tissue labeling Ago2 / eIF2C2 with ab186733 at 1/100 dilution followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.
Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
- WB
Lab
Western blot - Anti-Argonaute-2 antibody [EPR10411] (AB186733)
Blocking and diluting buffer and concentration : 5% NFDM/TBST.
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) (1 : 200000) (36KDa).
All lanes:
Western blot - Anti-AGO2(phospho S387) antibody [EPR29226-168] (ab326569) at 1/1000 dilution
Lane 1:
HEK293T cells were co-transfected with human AGO2(S387A) (His-tagged) and human AKT3 (EGFP-tagged) expression vectors, an upstream kinase used to enhance AGO2 phosphorylation at S387, whole cell lysate 20 µg (Untreated membrane)
Lane 2:
HEK293T cells were co-transfected with human AGO2 (His-tagged) and human AKT3 (EGFP-tagged) expression vectors, an upstream kinase used to enhance AGO2 phosphorylation at S387, whole cell lysate 20 µg (Untreated membrane)
Lane 3:
HEK293T cells were co-transfected with human AGO2 (His-tagged) and human AKT3 (EGFP-tagged) expression vectors, an upstream kinase used to enhance AGO2 phosphorylation at S387, whole cell lysate 20 µg (Alkaline phosphatase treated membrane)
Secondary
All lanes:
Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution
Observed band size: 97 kDa,36 kDa,75 kDa
false
Exposure time: 10s
- WB
Lab
Western blot - Anti-Argonaute-2 antibody [EPR10411] (AB186733)
False colour image of Western blot : Anti-Argonaute-2 antibody [EPR10411] staining at 1/1000 dilution, shown in green; Mouse anti-GAPDH antibody [6C5] (ab8245) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab186733 was shown to bind specifically to Argonaute-2. A band was observed at 95 kDa in wild-type HCT 116 cell lysates with no signal observed at this size in AGO2 knockout cell line. To generate this image, wild-type and AGO2 knockout HCT 116 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 5% milk in TBS-0.1% Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4°C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution.
All lanes:
Western blot - Anti-Argonaute-2 antibody [EPR10411] (ab186733) at 1/1000 dilution
Lane 1:
Wild-type HCT 116 cell lysate at 20 µg
Lane 2:
AGO2 knockout HCT 116 cell lysate at 20 µg
Lane 3:
U-87 MG cell lysate at 20 µg
Lane 4:
MCF7 cell lysate at 2 µg
Observed band size: 95 kDa
false
- WB
Supplier Data
Western blot - Anti-Argonaute-2 antibody [EPR10411] (AB186733)
All lanes:
Western blot - Anti-Argonaute-2 antibody [EPR10411] (ab186733) at 1/1000 dilution
Lane 1:
C6 cell lysate at 10 µg
Lane 2:
RAW 264.7 cell lysate at 10 µg
Secondary
All lanes:
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Predicted band size: 97 kDa
Observed band size: 97 kDa
false
- WB
Supplier Data
Western blot - Anti-Argonaute-2 antibody [EPR10411] (AB186733)
All lanes:
Western blot - Anti-Argonaute-2 antibody [EPR10411] (ab186733) at 1/1000 dilution
Lane 1:
HeLa cell lysate at 20 µg
Lane 2:
MCF7 cell lysate at 20 µg
Lane 3:
HepG2 cell lysate at 20 µg
Secondary
All lanes:
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Predicted band size: 97 kDa
Observed band size: 97 kDa
false
Related conjugates and formulations (10)
-
Anti-Argonaute-2 antibody [EPR10411] - BSA and Azide free
-
660 APC
APC Anti-Argonaute-2 antibody [EPR10411]
-
519 Alexa Fluor® 488
Alexa Fluor® 488 Anti-Argonaute-2 antibody [EPR10411]
-
565 Alexa Fluor® 555
Alexa Fluor® 555 Anti-Argonaute-2 antibody [EPR10411]
-
603 Alexa Fluor® 568
Alexa Fluor® 568 Anti-Argonaute-2 antibody [EPR10411]
-
617 Alexa Fluor® 594
Alexa Fluor® 594 Anti-Argonaute-2 antibody [EPR10411]
-
665 Alexa Fluor® 647
Alexa Fluor® 647 Anti-Argonaute-2 antibody [EPR10411]
-
775 Alexa Fluor® 750
Alexa Fluor® 750 Anti-Argonaute-2 antibody [EPR10411]
-
HRP Anti-Argonaute-2 antibody [EPR10411]
-
578 PE
PE Anti-Argonaute-2 antibody [EPR10411]
Reactivity data
Product details
What is this antibody validated in?
Anti-Argonaute-2 antibody [EPR10411] (ab186733) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Intra), Flow Cytometry (Flow Cyt), Immunoprecipitation (IP), Immunohistochemistry (IHC-P), Immunocytochemistry/immunofluorescence (ICC/IF) in Human, Mouse, Rat samples.
What is the molecular weight of Argonaute-2?
Anti-Argonaute-2 [EPR10411] (ab186733) specifically detects a band for Argonaute-2 (UniProt: Q9UKV8) at a molecular weight of 97kDa.
Trusted by the scientific community
Anti-Argonaute-2 [EPR10411] (ab186733) was first used in a scientific publication in 2014 and has been cited over 230 times in peer-reviewed journals.
Trial sizes available!
Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies.
Specificity confirmed
The specificity of Anti-Argonaute-2 antibody [EPR10411] (ab186733) has been confirmed by Western blot testing in AGO2 Knockout HCT 116 cells.
Other related products
We have a range of other formats of antibody clone [EPR10411] also available for your convenience: ab186733, Alexa Fluor® 488 - ab206104, Carrier free - ab233727, PE - ab305668, APC - ab305669, HRP - ab305670, Alkaline Phosphatase - ab308777, Alexa Fluor® 647 - ab310088, Alexa Fluor® 594 - ab310492, Alexa Fluor® 555 - ab312021, Alexa Fluor® 568 - ab312498, Alexa Fluor® 750 - ab321299
Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Properties and storage information
Form
Purification technique
Storage buffer
Shipped at conditions
Appropriate short-term storage duration
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Aliquoting information
Storage information
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
The Argonaute-2 protein participates in gene silencing mechanisms. As a part of RISC Ago2 binds to microRNAs (miRNAs) or small interfering RNAs (siRNAs) to direct sequence-specific cleavage of target mRNAs regulating their translation and stability. This protein is a part of a multi-protein complex that modulates gene expression and influences cellular homeostasis and development. In its biological role Argonaute-2 impacts cellular differentiation proliferation and apoptosis.
Pathways
Several cellular processes require Argonaute-2 notably the RNA interference (RNAi) and microRNA (miRNA) pathways. In these pathways it interacts with Dicer a protein essential for the maturation of small RNA molecules and facilitates the gene silencing process. Ago2 is also involved in the regulation of gene expression during embryonic development and stress responses indicating its importance in maintaining cellular function and responding to environmental changes.
Product protocols
- Visit the General protocols
- Visit the Troubleshooting
Target data
Publications (393)
Recent publications for all applications. Explore the full list and refine your search
Molecular medicine reports 32: PubMed41041848
2025
Applications
Unspecified application
Species
Unspecified reactive species
Translational stroke research 16:2211-2231 PubMed40956561
2025
Applications
Unspecified application
Species
Unspecified reactive species
Cell stress & chaperones 30:100113 PubMed40935014
2025
Applications
Unspecified application
Species
Unspecified reactive species
Oncology letters 30:459 PubMed40776904
2025
Applications
Unspecified application
Species
Unspecified reactive species
Scientific reports 15:24858 PubMed40640307
2025
Applications
Unspecified application
Species
Unspecified reactive species
Extracellular vesicle 5: PubMed40524753
2025
Applications
Unspecified application
Species
Unspecified reactive species
Journal of molecular histology 56:172 PubMed40419838
2025
Applications
Unspecified application
Species
Unspecified reactive species
Cell death discovery 11:238 PubMed40374610
2025
Applications
Unspecified application
Species
Unspecified reactive species
Cell biology and toxicology 41:79 PubMed40316855
2025
Applications
Unspecified application
Species
Unspecified reactive species
Discover oncology 16:640 PubMed40301147
2025
Applications
Unspecified application
Species
Unspecified reactive species
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com