AB233727

Anti-Argonaute-2 antibody [EPR10411] - BSA and Azide free

RabMAb
KO Validated
Recombinant
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(13 Publications)

Rabbit Recombinant Monoclonal Argonaute-2 antibody. Carrier free. Suitable for IP, WB, ICC/IF, Flow Cyt (Intra), IHC-P and reacts with Human, Mouse, Rat samples. Cited in 13 publications.

View Alternative Names

EIF2C2, AGO2, Protein argonaute-2, Argonaute2, hAgo2, Argonaute RISC catalytic component 2, Eukaryotic translation initiation factor 2C 2, PAZ Piwi domain protein, Protein slicer, eIF-2C 2, eIF2C 2, PPD

6 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Argonaute-2 antibody [EPR10411] - BSA and Azide free (AB233727)

IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Argonaute-2 antibody [EPR10411] - BSA and Azide free (AB233727)

This data was developed using ab186733, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of formalin fixed paraffin embedded human colon AC labelling Argonaute-2 with ab186733 at a concentration of 0.5 μg/ml. The immunostaining was performed on a Ventana DISCOVERY ULTRA (Roche Tissue Diagnostics) instrument with a OptiView DAB IHC Detection Kit. The heat-mediated antigen retrieval was conducted with at 100 °C, pH 8.5 for 32 mins. ab186733 Anti-Argonaute-2 antibody [EPR10411] was incubated for 16 mins, at 37 °C. Sections were counterstained with Hematoxylin II. Image inset shows absence of staining in secondary antibody only control. Customers are encouraged to optimise antigen retrieval conditions, antibody concentration, incubation times and temperature for best results in their own IHC assay workflow (automated and manual).

Flow Cytometry (Intracellular) - Anti-Argonaute-2 antibody [EPR10411] - BSA and Azide free (AB233727)

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-Argonaute-2 antibody [EPR10411] - BSA and Azide free (AB233727)

Intracellular flow cytometric analysis of 2% paraformaldehyde-fixed MCF7 cells labeling Ago2 / eIF2C2 with ab186733 at 1/60 dilution (red) compared to a Rabbit monoclonal IgG isotype control (green), followed by Goat anti rabbit IgG (FITC) secondary antibody at 1/150 dilution.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab186733).

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Argonaute-2 antibody [EPR10411] - BSA and Azide free (AB233727)

Immunohistochemical analysis of paraffin-embedded Human cervix carcinoma tissue labeling Ago2 / eIF2C2 with ab186733 at 1/100 dilution followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab186733).

Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

Immunocytochemistry/ Immunofluorescence - Anti-Argonaute-2 antibody [EPR10411] - BSA and Azide free (AB233727)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Argonaute-2 antibody [EPR10411] - BSA and Azide free (AB233727)

Immunofluorescent analysis of 4% paraformaldehyde-fixed HeLa cells labeling Ago2 / eIF2C2 with ab186733 at 1/250 dilution followed by Goat anti rabbit IgG (Alexa Fluor® 488) secondary antibody at 1/200 dilution. Counter stained with Dapi.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab186733).

Supplier Data

Immunoprecipitation - Anti-Argonaute-2 antibody [EPR10411] - BSA and Azide free (AB233727)

Western blot analysis of Ago2 / eIF2C2 in MCF7 cell lysate immunoprecipitated with ab186733 at 1/50 dilution.

Secondary antibody : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab186733).

All lanes:

Immunoprecipitation - Anti-Argonaute-2 antibody [EPR10411] (<a href='/en-us/products/primary-antibodies/argonaute-2-antibody-epr10411-ab186733'>ab186733</a>)

Predicted band size: 97 kDa

false

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Argonaute-2 antibody [EPR10411] - BSA and Azide free (AB233727)

Immunohistochemical analysis of paraffin-embedded Mouse kidney tissue labeling Ago2 / eIF2C2 with ab186733 at 1/100 dilution followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab186733).

Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

Unconjugated

Anti-Argonaute-2 antibody [EPR10411]
775 Alexa Fluor® 750

Alexa Fluor® 750 Anti-Argonaute-2 antibody [EPR10411]
519 Alexa Fluor® 488

Alexa Fluor® 488 Anti-Argonaute-2 antibody [EPR10411]
578 PE

PE Anti-Argonaute-2 antibody [EPR10411]
660 APC

APC Anti-Argonaute-2 antibody [EPR10411]
HRP

HRP Anti-Argonaute-2 antibody [EPR10411]
665 Alexa Fluor® 647

Alexa Fluor® 647 Anti-Argonaute-2 antibody [EPR10411]
617 Alexa Fluor® 594

Alexa Fluor® 594 Anti-Argonaute-2 antibody [EPR10411]
565 Alexa Fluor® 555

Alexa Fluor® 555 Anti-Argonaute-2 antibody [EPR10411]
603 Alexa Fluor® 568

Alexa Fluor® 568 Anti-Argonaute-2 antibody [EPR10411]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR10411

Isotype

IgG

Carrier free

Yes

Reacts with

Human, Mouse, Rat

Applications

Flow Cyt (Intra), IP, WB, ICC/IF, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-low-endotoxin-azide-free-ab199376'>ab199376</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Product details

ab233727 is the carrier-free version of ab186733.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Argonaute-2 often referred to as Ago2 or Argonaute-2 protein plays an important role in the RNA-induced silencing complex (RISC). It has a molecular weight of approximately 97 kDa. Ago2 is found in the cytoplasm and nucleus of cells and is expressed in a wide range of tissues including the brain and liver. Its mechanical function involves binding to guide RNA and facilitating strand cleavage which is essential for gene silencing and regulation at the post-transcriptional level.

Biological function summary

The Argonaute-2 protein participates in gene silencing mechanisms. As a part of RISC Ago2 binds to microRNAs (miRNAs) or small interfering RNAs (siRNAs) to direct sequence-specific cleavage of target mRNAs regulating their translation and stability. This protein is a part of a multi-protein complex that modulates gene expression and influences cellular homeostasis and development. In its biological role Argonaute-2 impacts cellular differentiation proliferation and apoptosis.

Pathways

Several cellular processes require Argonaute-2 notably the RNA interference (RNAi) and microRNA (miRNA) pathways. In these pathways it interacts with Dicer a protein essential for the maturation of small RNA molecules and facilitates the gene silencing process. Ago2 is also involved in the regulation of gene expression during embryonic development and stress responses indicating its importance in maintaining cellular function and responding to environmental changes.

Anomalies in Argonaute-2 activity correlate with cancer including lung and breast cancer where dysregulation of miRNA pathways can lead to unchecked cellular proliferation. It is also linked to neurological disorders like schizophrenia possibly due to its role in gene expression regulation in neural cells. Interactions between Ago2 and proteins like Dicer and other Argonautes suggest complex regulatory networks that when disrupted contribute to these diseases.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Required for RNA-mediated gene silencing (RNAi) by the RNA-induced silencing complex (RISC). The 'minimal RISC' appears to include AGO2 bound to a short guide RNA such as a microRNA (miRNA) or short interfering RNA (siRNA). These guide RNAs direct RISC to complementary mRNAs that are targets for RISC-mediated gene silencing. The precise mechanism of gene silencing depends on the degree of complementarity between the miRNA or siRNA and its target. Binding of RISC to a perfectly complementary mRNA generally results in silencing due to endonucleolytic cleavage of the mRNA specifically by AGO2. Binding of RISC to a partially complementary mRNA results in silencing through inhibition of translation, and this is independent of endonuclease activity. May inhibit translation initiation by binding to the 7-methylguanosine cap, thereby preventing the recruitment of the translation initiation factor eIF4-E. May also inhibit translation initiation via interaction with EIF6, which itself binds to the 60S ribosomal subunit and prevents its association with the 40S ribosomal subunit. The inhibition of translational initiation leads to the accumulation of the affected mRNA in cytoplasmic processing bodies (P-bodies), where mRNA degradation may subsequently occur. In some cases RISC-mediated translational repression is also observed for miRNAs that perfectly match the 3' untranslated region (3'-UTR). Can also up-regulate the translation of specific mRNAs under certain growth conditions. Binds to the AU element of the 3'-UTR of the TNF mRNA and up-regulates translation under conditions of serum starvation. Also required for transcriptional gene silencing (TGS), in which short RNAs known as antigene RNAs or agRNAs direct the transcriptional repression of complementary promoter regions.. (Microbial infection) Upon Sars-CoV-2 infection, associates with viral miRNA-like small RNA, CoV2-miR-O7a, and may repress mRNAs, such as BATF2, to evade the IFN response.

See full target information AGO2

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Publications (13)

Recent publications for all applications. Explore the full list and refine your search

Journal of dental sciences 20:1579-1589 PubMed40654486

2025

A novel NUTM2A-AS1/miR-769-5p axis regulates LPS-evoked damage in human dental pulp cells via the TLR4/MYD88/NF-κB signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Jing Li,Zeru Wang

Journal of biochemical and molecular toxicology 38:e70034 PubMed39555732

2024

Identification of the Oncogenic Role of the Circ_0001326/miR-577/VDAC1 Cascade in Prostate Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Zhirong Zhu,Guiliang Tang,Mengqi Shi,Mengjie Fang,Xiaolong Zhang,Huali Xu

Brain : a journal of neurology 148:1212-1227 PubMed39391934

2024

Dysregulation of protein SUMOylation networks in Huntington's disease R6/2 mouse striatum.

Applications

Unspecified application

Species

Unspecified reactive species

Marketta Kachemov,Vineet Vaibhav,Charlene Smith,Niveda Sundararaman,Marie Heath,Devon F Pendlebury,Andrea Matlock,Alice Lau,Eva Morozko,Ryan G Lim,Jack Reidling,Joan S Steffan,Jennifer E Van Eyk,Leslie M Thompson

Journal of biochemical and molecular toxicology 38:e23772 PubMed39030862

2024

CircCOL5A1 is involved in proliferation, invasion, and inhibition of ferroptosis of colorectal cancer cells via miR-1287-5p/SLC7A11.

Applications

Unspecified application

Species

Unspecified reactive species

Junwei Wang,Zili Zhang,Jianbin Zhuang,Di Kang,Weiliang Song

Journal of diabetes investigation 14:1344-1355 PubMed37688345

2023

Exosomal circHIPK3 derived from umbilical cord-derived mesenchymal stem cells enhances skin fibroblast autophagy by blocking miR-20b-5p/ULK1/Atg13 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Zhi-Yang Qiu,Shi-Shuai Lin,Nan-Fang Pan,Zhi-Hu Lin,Yun-Chuan Pan,Zun-Hong Liang

Journal of dental sciences 18:310-321 PubMed36643256

2023

Anti-apoptosis and anti-inflammation activity of circ_0097010 downregulation in lipopolysaccharide-stimulated periodontal ligament cells by miR-769-5p/Krüppel like factor 6 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Dan-Dan Sun,Xue Wu,Shi-Chen Lin,Shao-Yu Duan

Cell biology international 47:63-74 PubMed36273414

2022

Knockdown of long noncoding RNA MIAT attenuates hypoxia-induced cardiomyocyte injury by regulating the miR-488-3p/Wnt/β-catenin pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Ming Zhang,Zhiling Zhang,Jie Hu,Shulan Zhou,Wenwei Ai

Molecular medicine reports 26: PubMed36177898

2022

LOC102553417 silencing facilitates the apoptosis of hepatic stellate cells via the miR‑30e/MTDH axis.

Applications

Unspecified application

Species

Unspecified reactive species

Wujun Wei,Cheng Lin,Rentong Hu,Jingjing Huang,Xiaohao Chen,Lv Zhou,Jiazhu Wei,Yi-Bin Deng,Chun-Fang Wang

Thoracic cancer 13:2223-2234 PubMed35790503

2022

A novel circ_0000654/miR-375/E2F3 ceRNA network in esophageal squamous cell carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Chunyu Luo,Xiaowei Zhao,Yuan Wang,Yanqiu Li,Tuo Wang,Shumin Li

International heart journal 63:578-590 PubMed35650158

2022

LncRNA SNHG15 Modulates Ischemia-Reperfusion Injury in Human AC16 Cardiomyocytes Depending on the Regulation of the miR-335-3p/TLR4/NF-κB Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Haibo Du,Lianqin Ding,Tian Zeng,Di Li,Li Liu

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Argonaute-2 antibody [EPR10411] - BSA and Azide free

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Argonaute-2 antibody [EPR10411] - BSA and Azide free (AB233727)

Flow Cytometry (Intracellular) - Anti-Argonaute-2 antibody [EPR10411] - BSA and Azide free (AB233727)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Argonaute-2 antibody [EPR10411] - BSA and Azide free (AB233727)

Immunocytochemistry/ Immunofluorescence - Anti-Argonaute-2 antibody [EPR10411] - BSA and Azide free (AB233727)

Immunoprecipitation - Anti-Argonaute-2 antibody [EPR10411] - BSA and Azide free (AB233727)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Argonaute-2 antibody [EPR10411] - BSA and Azide free (AB233727)

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Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (13)

Product promise