Rabbit Recombinant Monoclonal Argonaute-2 antibody. Carrier free. Suitable for IP, WB, ICC/IF, Flow Cyt (Intra), IHC-P and reacts with Human, Mouse, Rat samples. Cited in 6 publications.
Images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Argonaute-2 antibody [EPR10411] - BSA and Azide free (AB233727), expandable thumbnail](https://content.abcam.com/products/images/argonaute-2-antibody-epr10411-bsa-and-azide-free-ab233727--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img56614.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Argonaute-2 antibody [EPR10411] - BSA and Azide free (AB233727)")
![Immunocytochemistry/ Immunofluorescence - Anti-Argonaute-2 antibody [EPR10411] - BSA and Azide free (AB233727), expandable thumbnail](https://content.abcam.com/products/images/argonaute-2-antibody-epr10411-bsa-and-azide-free-ab233727--immunocytochemistry-immunofluorescence-img2036.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-Argonaute-2 antibody [EPR10411] - BSA and Azide free (AB233727)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Argonaute-2 antibody [EPR10411] - BSA and Azide free (AB233727), expandable thumbnail](https://content.abcam.com/products/images/argonaute-2-antibody-epr10411-bsa-and-azide-free-ab233727--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img89523.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Argonaute-2 antibody [EPR10411] - BSA and Azide free (AB233727)")
![Immunoprecipitation - Anti-Argonaute-2 antibody [EPR10411] - BSA and Azide free (AB233727), expandable thumbnail](https://content.abcam.com/products/images/argonaute-2-antibody-epr10411-bsa-and-azide-free-ab233727--immunoprecipitation-img71860.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-Argonaute-2 antibody [EPR10411] - BSA and Azide free (AB233727)")
![Flow Cytometry (Intracellular) - Anti-Argonaute-2 antibody [EPR10411] - BSA and Azide free (AB233727), expandable thumbnail](https://content.abcam.com/products/images/argonaute-2-antibody-epr10411-bsa-and-azide-free-ab233727--flow-cytometry-intracellular-img55874.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-Argonaute-2 antibody [EPR10411] - BSA and Azide free (AB233727)")
Publications
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- Journal of dental sciences 18:310-3212023Anti-apoptosis and anti-inflammation activity of circ_0097010 downregulation in lipopolysaccharide-stimulated periodontal ligament cells by miR-769-5p/Krüppel like factor 6 axis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDan-Dan Sun,Xue Wu,Shi-Chen Lin,Shao-Yu DuanPubMed 36643256
- Cell biology international 47:63-742022Knockdown of long noncoding RNA MIAT attenuates hypoxia-induced cardiomyocyte injury by regulating the miR-488-3p/Wnt/β-catenin pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMing Zhang,Zhiling Zhang,Jie Hu,Shulan Zhou,Wenwei AiPubMed 36273414
- Molecular medicine reports 26:2022LOC102553417 silencing facilitates the apoptosis of hepatic stellate cells via the miR‑30e/MTDH axis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWujun Wei,Cheng Lin,Rentong Hu,Jingjing Huang,Xiaohao Chen,Lv Zhou,Jiazhu Wei,Yi-Bin Deng,Chun-Fang WangPubMed 36177898
- Thoracic cancer 13:2223-22342022A novel circ_0000654/miR-375/E2F3 ceRNA network in esophageal squamous cell carcinoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChunyu Luo,Xiaowei Zhao,Yuan Wang,Yanqiu Li,Tuo Wang,Shumin LiPubMed 35790503
- Cytotechnology 73:629-6422021Silencing of circHIPK3 hampers platelet-derived growth factor-induced proliferation and migration in airway smooth muscle cells through the miR-375/MMP-16 axis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYu Jiang,Xiaoqing Guo,Junhong QinPubMed 34349352
- Cancer management and research 13:1673-16862021Circ_0006174 Accelerates Colorectal Cancer Progression Through Regulating miR-138-5p/MACC1 Axis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJianjun Wei,Yuzhe Lin,Zhiqiang Wang,Yeguang Liu,Wei GuoPubMed 33628056
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IP | WB | ICC/IF | Flow Cyt (Intra) | IHC-P | |
|---|---|---|---|---|---|
| Human | Tested | Expected | Tested | Tested | Tested |
| Mouse | Predicted | Expected | Predicted | Predicted | Predicted |
| Rat | Predicted | Expected | Predicted | Predicted | Predicted |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
WB
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info Use at an assay dependent concentration. | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes ab199376 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Associated Products
Select an associated product type
9 products for Alternative Product
8 products for Alternative Version
Target data
Function
Required for RNA-mediated gene silencing (RNAi) by the RNA-induced silencing complex (RISC). The 'minimal RISC' appears to include AGO2 bound to a short guide RNA such as a microRNA (miRNA) or short interfering RNA (siRNA). These guide RNAs direct RISC to complementary mRNAs that are targets for RISC-mediated gene silencing. The precise mechanism of gene silencing depends on the degree of complementarity between the miRNA or siRNA and its target. Binding of RISC to a perfectly complementary mRNA generally results in silencing due to endonucleolytic cleavage of the mRNA specifically by AGO2. Binding of RISC to a partially complementary mRNA results in silencing through inhibition of translation, and this is independent of endonuclease activity. May inhibit translation initiation by binding to the 7-methylguanosine cap, thereby preventing the recruitment of the translation initiation factor eIF4-E. May also inhibit translation initiation via interaction with EIF6, which itself binds to the 60S ribosomal subunit and prevents its association with the 40S ribosomal subunit. The inhibition of translational initiation leads to the accumulation of the affected mRNA in cytoplasmic processing bodies (P-bodies), where mRNA degradation may subsequently occur. In some cases RISC-mediated translational repression is also observed for miRNAs that perfectly match the 3' untranslated region (3'-UTR). Can also up-regulate the translation of specific mRNAs under certain growth conditions. Binds to the AU element of the 3'-UTR of the TNF (TNF-alpha) mRNA and up-regulates translation under conditions of serum starvation. Also required for transcriptional gene silencing (TGS), in which short RNAs known as antigene RNAs or agRNAs direct the transcriptional repression of complementary promoter regions. (Microbial infection) Upon Sars-CoV-2 infection, associates with viral miRNA-like small RNA, CoV2-miR-O7a, and may repress mRNAs, such as BATF2, to evade the IFN response.
Alternative names
EIF2C2, AGO2, Protein argonaute-2, Argonaute2, hAgo2, Argonaute RISC catalytic component 2, Eukaryotic translation initiation factor 2C 2, PAZ Piwi domain protein, Protein slicer, eIF-2C 2, eIF2C 2, PPD
Recommended products
Rabbit Recombinant Monoclonal Argonaute-2 antibody. Carrier free. Suitable for IP, WB, ICC/IF, Flow Cyt (Intra), IHC-P and reacts with Human, Mouse, Rat samples. Cited in 6 publications.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR10411
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab233727 is the carrier-free version of Anti-Argonaute-2 antibody [EPR10411] ab186733.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Argonaute-2 often referred to as Ago2 or Argonaute-2 protein plays an important role in the RNA-induced silencing complex (RISC). It has a molecular weight of approximately 97 kDa. Ago2 is found in the cytoplasm and nucleus of cells and is expressed in a wide range of tissues including the brain and liver. Its mechanical function involves binding to guide RNA and facilitating strand cleavage which is essential for gene silencing and regulation at the post-transcriptional level.
- Biological function summary
The Argonaute-2 protein participates in gene silencing mechanisms. As a part of RISC Ago2 binds to microRNAs (miRNAs) or small interfering RNAs (siRNAs) to direct sequence-specific cleavage of target mRNAs regulating their translation and stability. This protein is a part of a multi-protein complex that modulates gene expression and influences cellular homeostasis and development. In its biological role Argonaute-2 impacts cellular differentiation proliferation and apoptosis.
- Pathways
Several cellular processes require Argonaute-2 notably the RNA interference (RNAi) and microRNA (miRNA) pathways. In these pathways it interacts with Dicer a protein essential for the maturation of small RNA molecules and facilitates the gene silencing process. Ago2 is also involved in the regulation of gene expression during embryonic development and stress responses indicating its importance in maintaining cellular function and responding to environmental changes.
- Associated diseases and disorders
Anomalies in Argonaute-2 activity correlate with cancer including lung and breast cancer where dysregulation of miRNA pathways can lead to unchecked cellular proliferation. It is also linked to neurological disorders like schizophrenia possibly due to its role in gene expression regulation in neural cells. Interactions between Ago2 and proteins like Dicer and other Argonautes suggest complex regulatory networks that when disrupted contribute to these diseases.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
5 product images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Argonaute-2 antibody [EPR10411] - BSA and Azide free (ab233727), expandable thumbnail](https://content.abcam.com/products/images/argonaute-2-antibody-epr10411-bsa-and-azide-free-ab233727--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img56614.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Argonaute-2 antibody [EPR10411] - BSA and Azide free (ab233727)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Argonaute-2 antibody [EPR10411] - BSA and Azide free (ab233727)
Immunohistochemical analysis of paraffin-embedded Human cervix carcinoma tissue labeling Ago2 / eIF2C2 with Anti-Argonaute-2 antibody [EPR10411] ab186733 at 1/100 dilution followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Argonaute-2 antibody [EPR10411] ab186733).
Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
![Immunocytochemistry/ Immunofluorescence - Anti-Argonaute-2 antibody [EPR10411] - BSA and Azide free (ab233727), expandable thumbnail](https://content.abcam.com/products/images/argonaute-2-antibody-epr10411-bsa-and-azide-free-ab233727--immunocytochemistry-immunofluorescence-img2036.jpg "Immunocytochemistry/ Immunofluorescence - Anti-Argonaute-2 antibody [EPR10411] - BSA and Azide free (ab233727)")
Immunocytochemistry/ Immunofluorescence - Anti-Argonaute-2 antibody [EPR10411] - BSA and Azide free (ab233727)
Immunofluorescent analysis of 4% paraformaldehyde-fixed HeLa cells labeling Ago2 / eIF2C2 with Anti-Argonaute-2 antibody [EPR10411] ab186733 at 1/250 dilution followed by Goat anti rabbit IgG (Alexa Fluor® 488) secondary antibody at 1/200 dilution. Counter stained with Dapi.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Argonaute-2 antibody [EPR10411] ab186733).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Argonaute-2 antibody [EPR10411] - BSA and Azide free (ab233727), expandable thumbnail](https://content.abcam.com/products/images/argonaute-2-antibody-epr10411-bsa-and-azide-free-ab233727--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img89523.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Argonaute-2 antibody [EPR10411] - BSA and Azide free (ab233727)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Argonaute-2 antibody [EPR10411] - BSA and Azide free (ab233727)
Immunohistochemical analysis of paraffin-embedded Mouse kidney tissue labeling Ago2 / eIF2C2 with Anti-Argonaute-2 antibody [EPR10411] ab186733 at 1/100 dilution followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Argonaute-2 antibody [EPR10411] ab186733).
Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
![Immunoprecipitation - Anti-Argonaute-2 antibody [EPR10411] - BSA and Azide free (ab233727), expandable thumbnail](https://content.abcam.com/products/images/argonaute-2-antibody-epr10411-bsa-and-azide-free-ab233727--immunoprecipitation-img71860.jpg "Immunoprecipitation - Anti-Argonaute-2 antibody [EPR10411] - BSA and Azide free (ab233727)")
Immunoprecipitation - Anti-Argonaute-2 antibody [EPR10411] - BSA and Azide free (ab233727)
Western blot analysis of Ago2 / eIF2C2 in MCF7 cell lysate immunoprecipitated with Anti-Argonaute-2 antibody [EPR10411] ab186733 at 1/50 dilution.
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Argonaute-2 antibody [EPR10411] ab186733).
All lanes: Immunoprecipitation - Anti-Argonaute-2 antibody [EPR10411] (Anti-Argonaute-2 antibody [EPR10411] ab186733)
Predicted band size: 97 kDa
![Flow Cytometry (Intracellular) - Anti-Argonaute-2 antibody [EPR10411] - BSA and Azide free (ab233727), expandable thumbnail](https://content.abcam.com/products/images/argonaute-2-antibody-epr10411-bsa-and-azide-free-ab233727--flow-cytometry-intracellular-img55874.jpg "Flow Cytometry (Intracellular) - Anti-Argonaute-2 antibody [EPR10411] - BSA and Azide free (ab233727)")
Flow Cytometry (Intracellular) - Anti-Argonaute-2 antibody [EPR10411] - BSA and Azide free (ab233727)
Intracellular flow cytometric analysis of 2% paraformaldehyde-fixed MCF7 cells labeling Ago2 / eIF2C2 with Anti-Argonaute-2 antibody [EPR10411] ab186733 at 1/60 dilution (red) compared to a Rabbit monoclonal IgG isotype control (green), followed by Goat anti rabbit IgG (FITC) secondary antibody at 1/150 dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Argonaute-2 antibody [EPR10411] ab186733).
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