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Rabbit Recombinant Monoclonal ARH antibody. Carrier free. Suitable for WB and reacts with Human, Mouse, Rat samples.

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Images

Western blot - Anti-ARH antibody [EPR13115] - BSA and Azide free (AB250304), expandable thumbnail
  • Western blot - Anti-ARH antibody [EPR13115] - BSA and Azide free (AB250304), expandable thumbnail
  • Western blot - Anti-ARH antibody [EPR13115] - BSA and Azide free (AB250304), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Constituents: PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPWB
Human
Not recommended
Tested
Mouse
Not recommended
Tested
Rat
Not recommended
Tested

Not recommended
Not recommended

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Target data

Function

Adapter protein (clathrin-associated sorting protein (CLASP)) required for efficient endocytosis of the LDL receptor (LDLR) in polarized cells such as hepatocytes and lymphocytes, but not in non-polarized cells (fibroblasts). May be required for LDL binding and internalization but not for receptor clustering in coated pits. May facilitate the endocytosis of LDLR and LDLR-LDL complexes from coated pits by stabilizing the interaction between the receptor and the structural components of the pits. May also be involved in the internalization of other LDLR family members. Binds to phosphoinositides, which regulate clathrin bud assembly at the cell surface. Required for trafficking of LRP2 to the endocytic recycling compartment which is necessary for LRP2 proteolysis, releasing a tail fragment which translocates to the nucleus and mediates transcriptional repression (By similarity).

Alternative names

ARH, LDLRAP1, Low density lipoprotein receptor adapter protein 1, Autosomal recessive hypercholesterolemia protein

Recommended products

Rabbit Recombinant Monoclonal ARH antibody. Carrier free. Suitable for WB and reacts with Human, Mouse, Rat samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free
Yes
Clone number
EPR13115
Purification technique
Affinity purification
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze

Notes

ab250304 is the carrier-free version of Anti-ARH antibody [EPR13115] - N-terminal ab181043.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

The ARH protein also known as autosomal recessive hypercholesterolemia protein plays an important role in the intracellular trafficking and endocytosis of low-density lipoprotein receptors (LDLR). ARH has a molecular mass of approximately 308 amino acids and is expressed primarily in the liver heart and adrenal gland. Its function is important in maintaining cholesterol homeostasis and influencing lipid metabolism by mediating receptor-ligand interactions.

Biological function summary

ARH facilitates the internalization of LDL particles by forming a complex with LDL receptors at the cell surface. This process helps in the uptake of cholesterol-rich lipoproteins an essential task for cell function. By binding adaptor proteins and clathrin ARH aids in creating pits that vesicles need during LDL endocytosis ensuring specific retrieval of receptors and ligands. This protein plays a role in maintaining cellular lipid balance and contributes to cholesterol regulation.

Pathways

ARH acts in the lipoprotein metabolism and cholesterol transport pathways by collaborating with other proteins. It interacts with LDLR and integrates into the clathrin-mediated endocytic pathway pivotal for cholesterol uptake and distribution within the body. It is associated with proteins such as LDLR and clathrin which perform key steps in lipoprotein processing and cholesterol homeostasis.

Associated diseases and disorders

Alterations in ARH can lead to hypercholesterolemia a disorder characterized by elevated cholesterol levels in the blood and increased cardiovascular risk. ARH is associated with familial hypercholesterolemia reflecting its central role in cholesterol regulation. The protein's interaction with LDLR is important in this context as defects in this interaction result in impaired clearance of LDL from the bloodstream. Such disruptions emphasize the importance of ARH in lipid-related disorders and potential therapeutic targeting.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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