AB199171

Anti-ARH3 antibody - C-terminal

Name: Anti-ARH3 antibody - C-terminal (ab199171) | Abcam
Brand: Abcam Limited
SKU: AB199171

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(1 Publication)

Rabbit Polyclonal ARH3 antibody. C-terminal. Suitable for WB and reacts with Mouse, Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human ADPRS.

View Alternative Names

ADPRHL2, ARH3, ADPRS, ADP-ribosylhydrolase ARH3, ADP-ribose glycohydrolase ARH3, ADP-ribosylhydrolase 3, O-acetyl-ADP-ribose deacetylase ARH3, Poly(ADP-ribose) glycohydrolase ARH3, [Protein ADP-ribosylarginine] hydrolase-like protein 2, [Protein ADP-ribosylserine] hydrolase

1 Images

Supplier Data

Western blot - Anti-ARH3 antibody - C-terminal (AB199171)

Gel : 12% SDS-PAGE

All lanes:

Western blot - Anti-ARH3 antibody - C-terminal (ab199171) at 1/500 dilution

Lane 1:

HeLa cell lysate at 40 µg

Lane 2:

Mouse kidney tissue lysate at 40 µg

Predicted band size: 39 kDa

true

Exposure time: 2min

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

applications

Immunogen

Synthetic Peptide within Human ADPRS. The exact immunogen used to generate this antibody is proprietary information.

Q9NX46

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/200 - 1/1000", "WB-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/200 - 1/1000", "WB-species-notes": "<p></p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.4 Preservative: 0.05% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ARH3 also known as ADP-ribosylhydrolase 3 is an enzyme involved in the removal of ADP-ribose groups from proteins a process known as de-ADP-ribosylation. It has a mass of approximately 39 kDa. The enzyme shows widespread expression in human tissues with higher levels in the brain heart and skeletal muscles. This expression pattern indicates its important role in various cellular processes across different tissue types.

Biological function summary

ARH3 catalyzes the hydrolysis of ADP-ribose units attached to serine residues on target proteins reversing the actions of PARPs (poly ADP-ribose polymerases) especially PARP1. By doing this it plays a role in regulating the post-translational modification of proteins. Although not considered a part of a permanent complex ARH3 often interacts with other protein factors involved in DNA repair and cellular stress responses suggesting its dynamic involvement in cellular regulation.

Pathways

This enzyme functions in DNA repair and cellular stress response pathways. ARH3's activity complements the work of PARP1 by removing ADP-ribose groups added during the DNA damage response. This enzymatic balance is essential to prevent excessive ADP-ribosylation that can affect DNA repair processes. The interaction between ARH3 and PARP1 illustrates the coordinated efforts required to maintain genomic stability and respond to cellular stress.

Mutations or dysregulation of ARH3 are linked to neurodegenerative disorders and cancer. In neurological diseases proteins modified by ARH3-driven de-ADP-ribosylation may alter neuronal survival and function. Similarly in cancer improper ARH3 activity could lead to uncontrolled cell proliferation due to a faulty DNA damage response. Tumor protein 53 (TP53) might interact with ARH3 indicating potential pathways affected in cancerous conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

ADP-ribosylhydrolase that preferentially hydrolyzes the scissile alpha-O-linkage attached to the anomeric C1'' position of ADP-ribose and acts on different substrates, such as proteins ADP-ribosylated on serine and threonine, free poly(ADP-ribose) and O-acetyl-ADP-D-ribose (PubMed : 21498885, PubMed : 29907568, PubMed : 30045870, PubMed : 30401461, PubMed : 30830864, PubMed : 33186521, PubMed : 33769608, PubMed : 33894202, PubMed : 34019811, PubMed : 34321462, PubMed : 34479984, PubMed : 34625544). Specifically acts as a serine mono-ADP-ribosylhydrolase by mediating the removal of mono-ADP-ribose attached to serine residues on proteins, thereby playing a key role in DNA damage response (PubMed : 28650317, PubMed : 29234005, PubMed : 30045870, PubMed : 33186521, PubMed : 34019811, PubMed : 34625544). Serine ADP-ribosylation of proteins constitutes the primary form of ADP-ribosylation of proteins in response to DNA damage (PubMed : 29480802, PubMed : 33186521, PubMed : 34625544). Does not hydrolyze ADP-ribosyl-arginine, -cysteine, -diphthamide, or -asparagine bonds (PubMed : 16278211, PubMed : 33769608). Also able to degrade protein free poly(ADP-ribose), which is synthesized in response to DNA damage : free poly(ADP-ribose) acts as a potent cell death signal and its degradation by ADPRHL2 protects cells from poly(ADP-ribose)-dependent cell death, a process named parthanatos (PubMed : 16278211). Also hydrolyzes free poly(ADP-ribose) in mitochondria (PubMed : 22433848). Specifically digests O-acetyl-ADP-D-ribose, a product of deacetylation reactions catalyzed by sirtuins (PubMed : 17075046, PubMed : 21498885). Specifically degrades 1''-O-acetyl-ADP-D-ribose isomer, rather than 2''-O-acetyl-ADP-D-ribose or 3''-O-acetyl-ADP-D-ribose isomers (PubMed : 21498885).

See full target information ADPRS

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Cancers 16: PubMed39518018

2024

Human Cripto-1 and Cripto-3 Protein Expression in Normal and Malignant Settings That Conflicts with Established Conventions.

Applications

Unspecified application

Species

Unspecified reactive species

Frank Cuttitta,Josune García-Sanmartín,Yang Feng,Mary Elizabeth Sunday,Young S Kim,Alfredo Martínez

View all publications

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Product promise

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Anti-ARH3 antibody - C-terminal

Western blot - Anti-ARH3 antibody - C-terminal (AB199171)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Product promise