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AB194425

Anti-ARHGAP1 antibody

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(1 Publication)

Mouse Polyclonal ARHGAP1 antibody. Suitable for WB and reacts with Human, Mouse, Rat samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human ARHGAP1 aa 300 to C-terminus.

View Alternative Names

CDC42GAP, RHOGAP1, ARHGAP1, Rho GTPase-activating protein 1, CDC42 GTPase-activating protein, GTPase-activating protein rhoGAP, Rho-related small GTPase protein activator, Rho-type GTPase-activating protein 1, p50-RhoGAP

6 Images
Western blot - Anti-ARHGAP1 antibody (AB194425)
  • WB

Supplier Data

Western blot - Anti-ARHGAP1 antibody (AB194425)

All lanes:

Western blot - Anti-ARHGAP1 antibody (ab194425) at 1/500 dilution

All lanes:

rat PC12 cell lysate at 50 µg

Predicted band size: 50 kDa

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Western blot - Anti-ARHGAP1 antibody (AB194425)
  • WB

Supplier Data

Western blot - Anti-ARHGAP1 antibody (AB194425)

All lanes:

Western blot - Anti-ARHGAP1 antibody (ab194425) at 1/500 dilution

All lanes:

mouse Raw 264.7 cell lysate at 50 µg

Predicted band size: 50 kDa

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Western blot - Anti-ARHGAP1 antibody (AB194425)
  • WB

Supplier Data

Western blot - Anti-ARHGAP1 antibody (AB194425)

All lanes:

Western blot - Anti-ARHGAP1 antibody (ab194425) at 1/500 dilution

All lanes:

MES-SA/Dx5 cell lysate at 50 µg

Predicted band size: 50 kDa

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Western blot - Anti-ARHGAP1 antibody (AB194425)
  • WB

Supplier Data

Western blot - Anti-ARHGAP1 antibody (AB194425)

All lanes:

Western blot - Anti-ARHGAP1 antibody (ab194425) at 1/500 dilution

All lanes:

Jurkat cell lysate at 50 µg

Predicted band size: 50 kDa

true

Western blot - Anti-ARHGAP1 antibody (AB194425)
  • WB

Supplier Data

Western blot - Anti-ARHGAP1 antibody (AB194425)

All lanes:

Western blot - Anti-ARHGAP1 antibody (ab194425) at 1/1000 dilution

All lanes:

recombinant ARHGAP1 protein fragment at 0.2 µg

Predicted band size: 50 kDa

true

Western blot - Anti-ARHGAP1 antibody (AB194425)
  • WB

Supplier Data

Western blot - Anti-ARHGAP1 antibody (AB194425)

All lanes:

Western blot - Anti-ARHGAP1 antibody (ab194425) at 1/500 dilution

All lanes:

mouse NIH 3T3 cell lysate at 50 µg

Predicted band size: 50 kDa

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Key facts

Host species

Mouse

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat, Human

Applications

WB

applications

Immunogen

Recombinant Fragment Protein within Human ARHGAP1 aa 300 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q07960

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2500", "WB-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2500", "WB-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2500", "WB-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purity
Whole antiserum
Storage buffer
Constituents: 50% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ARHGAP1 also known as Rho GTPase-activating protein 1 plays an important role in the regulation of the Rho family of GTPases. These proteins control the actin cytoskeleton affecting cell shape and migration. ARHGAP1 acts as a GTPase-activating protein stimulating the GTPase activity of Rho-type GTPases and thereby controls their signaling activity. The protein weighs about 48 kDa and shows expression in various tissues including lung heart and liver.
Biological function summary

ARHGAP1 contributes to cell movement processes such as migration and invasion. It is part of a regulatory complex that modulates the signaling pathways involving actin cytoskeletal dynamics. ARHGAP1 interacts with key molecules in pathways that manage cell adhesion and motility indicating its role in cellular dynamics and structural integrity.

Pathways

ARHGAP1 actively participates in the Rho GTPase cycle interacting with proteins like Cdc42 and Rac which are involved in controlling actin polymerization dynamics. The protein plays a role in pathways like the VEGF signaling pathway influencing how cells respond to growth signals. It provides an intersection between small GTPase signaling and cytoskeletal rearrangement essential for cell movement and stability.

ARHGAP1 shows associations with cancer progression and metastasis. Changes in ARHGAP1 expression can influence the behavior of cancer cells increasing their migratory and invasive capabilities. In addition ARHGAP1 connects with the protein EGFR where aberrant pathways involving these proteins can lead to non-small cell lung cancer. In this context it is important to further investigate ARHGAP1's roles to understand its potential use in therapeutic developments.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

GTPase activator for the Rho, Rac and Cdc42 proteins, converting them to the putatively inactive GDP-bound state. Cdc42 seems to be the preferred substrate.
See full target information ARHGAP1
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

OncoTargets and therapy 10:691-701 PubMed28223826

2017

ARHGAP1 overexpression inhibits proliferation, migration and invasion of C-33A and SiHa cell lines.

Applications

WB

Species

Human

Jun-Ping Li,Yang Liu,Yi-Hua Yin
View all publications
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Product promise

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