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AB222805

Anti-ARHGAP10 antibody

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(4 Publications)

Rabbit Polyclonal ARHGAP10 antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Cited in 4 publications. Immunogen corresponding to Recombinant Fragment Protein within Human ARHGAP10 aa 500-750.

View Alternative Names

GRAF2, ARHGAP10, Rho GTPase-activating protein 10, GTPase regulator associated with focal adhesion kinase 2, Graf-related protein 2, Rho-type GTPase-activating protein 10

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARHGAP10 antibody (AB222805)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARHGAP10 antibody (AB222805)

Paraffin-embedded human lung cancer tissue stained for ARHGAP10 using ab222805 at 1/100 dilution in immunohistochemical analysis.

Immunocytochemistry/ Immunofluorescence - Anti-ARHGAP10 antibody (AB222805)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-ARHGAP10 antibody (AB222805)

HepG2 (human liver hepatocellular carcinoma cell line) cells stained for ARHGAP10 (green) using ab222805 at 1/100 dilution in ICC/IF, followed by Alexa Fluor 488® conjugated Goat Anti-Rabbit IgG (H+L).

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

ICC/IF, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human ARHGAP10 aa 500-750. The exact immunogen used to generate this antibody is proprietary information.

A1A4S6

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/20 - 1/200", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50 - 1/200", "ICCIF-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Purification notes
Purity >95%.
Storage buffer
pH: 7.4 Preservative: 0.03% Proclin 300 Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ARHGAP10 also known as Rho GTPase activating protein 10 is involved in regulating the activity of Rho-type GTPases. This protein acts as a GTPase-activating protein (GAP) promoting the inactivation of RhoA Rac1 and Cdc42 molecules. ARHGAP10 weighs approximately 88 kDa and shows high expression levels in the brain and the lymphoid tissues. Its activity influences cellular mechanisms through the modulation of the actin cytoskeleton affecting various cellular processes such as movement and morphology.
Biological function summary

The function of ARHGAP10 involves the modulation of cytoskeletal dynamics linking it directly to processes of cellular movement and structure. It participates as part of a complex network that influences cell signaling and cytoskeletal rearrangement. Through its interaction with Rho family GTPases ARHGAP10 plays a significant role in altering cellular adhesion and migration essential processes in development and tissue maintenance.

Pathways

ARHGAP10 is prominently involved in the Rho GTPase signaling pathway influencing actin cytoskeleton organization. It interacts with proteins like ROCK1 and ROCK2 affecting contraction in smooth muscle cells. Another critical pathway involving ARHGAP10 includes the Wnt signaling pathway where it influences cellular proliferation and differentiation impacting developmental processes and cellular responses to external signals.

ARHGAP10 has a connection to cancer particularly through its role in cellular migration and invasion. Abnormal regulation of ARHGAP10 expression or function could lead to dysregulated cell movement contributing to metastasis. Furthermore ARHGAP10's involvement in the Rho GTPase pathway links it to neurodevelopmental disorders as it affects the organization of neural structures. In cancer proteins like Rac1 and Cdc42 which interact with ARHGAP10 are often implicated in the modulation of tumor progression and metastasis.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

GTPase-activating protein that catalyzes the conversion of active GTP-bound Rho GTPases to their inactive GDP-bound form, thus suppressing various Rho GTPase-mediated cellular processes (PubMed : 11432776). Also converts Cdc42 to an inactive GDP-bound state (PubMed : 11432776). Essential for PTKB2 regulation of cytoskeletal organization via Rho family GTPases. Inhibits PAK2 proteolytic fragment PAK-2p34 kinase activity and changes its localization from the nucleus to the perinuclear region. Stabilizes PAK-2p34 thereby increasing stimulation of cell death (By similarity). Associates with MICAL1 on the endosomal membrane to promote Rab8-Rab10-dependent tubule extension. After dissociation with MICAL1, recruits WDR44 which connects the endoplasmic reticulum (ER) with the endosomal tubule, thereby participating in the export of a subset of neosynthesized proteins (PubMed : 32344433).
See full target information ARHGAP10
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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Cell research 33:30-45 PubMed36241718

2022

A novel protein RASON encoded by a lncRNA controls oncogenic RAS signaling in KRAS mutant cancers.

Applications

Unspecified application

Species

Unspecified reactive species

Rongjie Cheng,Fanying Li,Maolei Zhang,Xin Xia,Jianzhuang Wu,Xinya Gao,Huangkai Zhou,Zhi Zhang,Nunu Huang,Xuesong Yang,Yaliang Zhang,Shunli Shen,Tiebang Kang,Zexian Liu,Feizhe Xiao,Hongwei Yao,Jianbo Xu,Chao Yan,Nu Zhang

Frontiers in cell and developmental biology 8:582220 PubMed33163494

2020

Protein Tyrosine Phosphatase Receptor S Acts as a Metastatic Suppressor in Malignant Peripheral Nerve Sheath Tumor via Profilin 1-Induced Epithelial-Mesenchymal Transition.

Applications

Unspecified application

Species

Unspecified reactive species

Jie-Yi Ren,Yi-Hui Gu,Xi-Wei Cui,Man-Mei Long,Wei Wang,Cheng-Jiang Wei,Bin Gu,Hai-Bing Zhang,Qing-Feng Li,Zhi-Chao Wang

Clinical and experimental pharmacology & physiology 47:1591-1599 PubMed32391922

2020

ARHGAP17 inhibits pathological cyclic strain-induced apoptosis in human periodontal ligament fibroblasts via Rac1/Cdc42.

Applications

Unspecified application

Species

Unspecified reactive species

Li Wang,Xiaojie Yang,Leilei Wan,Shiwei Wang,Jinsong Pan,Yuehua Liu

International journal of clinical and experimental 12:3839-3846 PubMed31933772

2019

Expression of ARHGAP10 correlates with prognosis of prostate cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Hua Gong,Xingyi Chen,Yongcao Jin,Jiasun Lu,Yuanjue Cai,Ouyang Wei,Jun Zhao,Wenyuan Zhang,Xiaofei Wen,Yuemin Wang,Weihua Chen
View all publications
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