AB106553

Anti-ARHGAP18 antibody

Name: Anti-ARHGAP18 antibody (ab106553) | Abcam
Brand: Abcam Limited
SKU: AB106553
Price: 490 USD
Availability: InStock

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(2 Publications)

Rabbit Polyclonal ARHGAP18 antibody. Suitable for ICC, WB, ICC/IF and reacts with Mouse samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human Rho GTPase-activating protein 18.

View Alternative Names

Rho GTPase-activating protein 18, MacGAP, Rho-type GTPase-activating protein 18, ARHGAP18

3 Images

Immunocytochemistry/ Immunofluorescence - Anti-ARHGAP18 antibody (AB106553)

ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-ARHGAP18 antibody (AB106553)

Immunofluorescence of ARHGAP18 in 3T3 cells using ab106553 at 20 ug/ml.

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Immunocytochemistry - Anti-ARHGAP18 antibody (AB106553)

ab106553 at 2.5μg/ml staining ARHGAP18 in 3T3 cells by Immunocytochemistry.

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Western blot - Anti-ARHGAP18 antibody (AB106553)

Lane 1:

Western blot - Anti-ARHGAP18 antibody (ab106553) at 1 µg/mL

Lane 2:

Western blot - Anti-ARHGAP18 antibody (ab106553) at 2 µg/mL

All lanes:

3T3 cell lysate at 15 µg

Predicted band size: 74 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse

Applications

WB, ICC, ICC/IF

applications

Immunogen

Synthetic Peptide within Human Rho GTPase-activating protein 18. The exact immunogen used to generate this antibody is proprietary information.

Q8N392

Specificity

At least two isoforms of ARHGAP18 are known to exist; ab106553 with detect both isoforms. ab106553 will not cross-react with other ARHGAP family members.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICC" : {"fullname" : "Immunocytochemistry", "shortname":"ICC"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "ICC-species-checked": "testedAndGuaranteed", "ICC-species-dilution-info": "2.5 µg/mL", "ICC-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1-2 µg/mL", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "20 µg/mL", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

Up to 12 months

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ARHGAP18 also known as Rho GTPase Activating Protein 18 is a protein that plays a role in regulating the actin cytoskeleton. It is part of the larger family of Rho GTPase-activating proteins (GAPs) and has a molecular mass of approximately 57 kDa. ARHGAP18 is expressed in various tissues including the liver and lung indicating its importance in different cellular contexts.

Biological function summary

ARHGAP18 influences cell shape and motility by modulating the Rho signaling pathway. It acts as a negative regulator of RhoA activity thereby impacting actin filament organization. The protein does not form large complexes but interacts with other cytoskeleton-associated proteins to exert its effects on cell structure and movement.

Pathways

ARHGAP18 is involved in the regulation of the RhoA signaling pathway which is essential for cell movement and growth. This protein interacts with other Rho GTPases to maintain cellular architecture and coordination during cellular processes. Notably it participates in pathways that control the dynamics of the actin cytoskeleton aligning with proteins such as ROCK1 and ROCK2.

ARHGAP18 has been linked to the progression of cancer and cardiovascular diseases. Abnormal expression of ARHGAP18 can lead to irregularities in cell migration and invasion contributing to cancer metastasis. In cardiovascular disorders it interacts with proteins such as RhoA and involves in the pathogenesis of atherosclerosis by affecting vascular smooth muscle cell function.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Rho GTPase activating protein that suppresses F-actin polymerization by inhibiting Rho. Rho GTPase activating proteins act by converting Rho-type GTPases to an inactive GDP-bound state (PubMed : 21865595). Plays a key role in tissue tension and 3D tissue shape by regulating cortical actomyosin network formation. Acts downstream of YAP1 and inhibits actin polymerization, which in turn reduces nuclear localization of YAP1 (PubMed : 25778702). Regulates cell shape, spreading, and migration (PubMed : 21865595).

See full target information Rho GTPase-activating protein 18

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Disease models & mechanisms 14: PubMed33973626

2021

Thiopurines correct the effects of autophagy impairment on intestinal healing - a potential role for ARHGAP18/RhoA.

Applications

Unspecified application

Species

Unspecified reactive species

Marileen M C Prins,Francesca P Giugliano,Manon van Roest,Stan F J van de Graaf,Pim J Koelink,Manon E Wildenberg

The Turkish journal of gastroenterology : the official journal of Turkish Society of Gastroenterology 30:290-298 PubMed30923033

2019

Curcumin ameliorates dextran sulfate sodium-induced colitis in mice via regulation of autophagy and intestinal immunity.

Applications

Unspecified application

Species

Unspecified reactive species

Wenjie Yue,Yi Liu,Xiang Li,Liyuan Lv,Jianping Huang,Jie Liu

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-ARHGAP18 antibody

Immunocytochemistry/ Immunofluorescence - Anti-ARHGAP18 antibody (AB106553)

Immunocytochemistry - Anti-ARHGAP18 antibody (AB106553)

Western blot - Anti-ARHGAP18 antibody (AB106553)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

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