Rabbit Polyclonal ARHGAP22 antibody. Suitable for IHC-P, WB and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human ARHGAP22 aa 450-600.
Images
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine)- Form
Liquid
- Clonality
Polyclonal
Immunogen
- Recombinant Fragment Protein within Human ARHGAP22 aa 450-600. The exact immunogen used to generate this antibody is proprietary information. Database link Q7Z5H3
Reactivity data
Select an application| IHC-P | WB | |
|---|---|---|
| Human | Tested | Tested |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/50.00000 - 1/200.00000 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 0.04000-0.40000 µg/mL | Notes - |
Target data
Function
Rho GTPase-activating protein involved in the signal transduction pathway that regulates endothelial cell capillary tube formation during angiogenesis. Acts as a GTPase activator for the RAC1 by converting it to an inactive GDP-bound state. Inhibits RAC1-dependent lamellipodia formation. May also play a role in transcription regulation via its interaction with VEZF1, by regulating activity of the endothelin-1 (EDN1) promoter (By similarity).
Alternative names
Rho GTPase-activating protein 22, Rho-type GTPase-activating protein 22, ARHGAP22, RHOGAP2
Recommended products
Rabbit Polyclonal ARHGAP22 antibody. Suitable for IHC-P, WB and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human ARHGAP22 aa 450-600.
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine)- Form
Liquid
- Clonality
Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human ARHGAP22 aa 450-600. The exact immunogen used to generate this antibody is proprietary information. Database link Q7Z5H3
- Purification technique
Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage duration
1-2 weeks
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
ARHGAP22 also known as Rho GTPase-activating protein 22 functions as a critical regulator of Rho family GTPases through GTPase activation which leads to the inactivation of RhoC and Cdc42. This protein includes the unique domains required for its GTPase-activating role and has a molecular mass of approximately 75 kDa. ARHGAP22 predominantly expresses in tissues such as the brain kidney and spleen.
- Biological function summary
ARHGAP22 modulates actin organization which affects cell shape and motility. It plays a role in the regulation of cell migration and cytoskeleton dynamics. Though not part of a well-defined multiprotein complex ARHGAP22 interacts with other proteins involved in cytoskeletal rearrangement supporting cellular communication and migration processes.
- Pathways
ARHGAP22 integrates into signaling cascades like the Rho GTPase cycle influencing cell migration pathways. It interacts with proteins such as RhoC and Cdc42 within these pathways contributing to dynamic cellular remodeling. The pathways linked to ARHGAP22 involve cytoskeletal reorganization affecting processes like angiogenesis and neural development.
- Associated diseases and disorders
ARHGAP22 has implications in cancer progression particularly through its role in metastasis. Overexpression or misregulation can contribute to enhanced cell migration and invasion leading to a more aggressive cancer phenotype. Additionally ARHGAP22 is connected to neurological disorders possibly through its impact on neural cell migration. Interaction with proteins such as Cdc42 can disrupt normal signaling leading to these pathological conditions.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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2 product images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARHGAP22 antibody (ab204743)
Immunohistochemical analysis of paraffin-embedded Human testis tissue labeling ARHGAP22 with ab204743 at 1/50 dilution.
Western blot - Anti-ARHGAP22 antibody (ab204743)
All lanes: Western blot - Anti-ARHGAP22 antibody (ab204743) at 1/100 dilution
All lanes: RT-4 cell lysate
Predicted band size: 77 kDa
Downloads
Product protocols
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