AB251103

Anti-ARHGAP25 antibody [EPR13233-60] - BSA and Azide free

RabMAb
Recombinant
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Rabbit Recombinant Monoclonal ARHGAP25 antibody. Carrier free. Suitable for WB, IHC-P and reacts with Human samples.

View Alternative Names

KIAA0053, ARHGAP25, Rho GTPase-activating protein 25, Rho-type GTPase-activating protein 25

4 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARHGAP25 antibody [EPR13233-60] - BSA and Azide free (AB251103)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARHGAP25 antibody [EPR13233-60] - BSA and Azide free (AB251103)

This data was developed using ab192020, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human stomach tissue labeling ARHGAP25 using ab192020 at 1/200 dilution (5 μg/mL). A prediluted HRP Polymer for Rabbit IgG was used as a secondary antibody. Counterstain : Hematoxylin. Inset image : negative control obtained using PBS instead of ab192020.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARHGAP25 antibody [EPR13233-60] - BSA and Azide free (AB251103)

This data was developed using ab192020, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human hepatocellular carcinoma tissue labeling ARHGAP25 using ab192020 at 1/200 dilution (5 μg/mL). A prediluted HRP Polymer for Rabbit IgG was used as a secondary antibody. Counterstain : Hematoxylin. Inset image : negative control obtained using PBS instead of ab192020.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Supplier Data

Western blot - Anti-ARHGAP25 antibody [EPR13233-60] - BSA and Azide free (AB251103)

This data was developed using ab192020, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-ARHGAP25 antibody [EPR13233-60] - BSA and Azide free (ab251103) at 1/10000 dilution

Lane 1:

Jurkat cell lysate at 20 µg

Lane 2:

Human tonsil tissue lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 73 kDa

Observed band size: 77 kDa

false

Supplier Data

Western blot - Anti-ARHGAP25 antibody [EPR13233-60] - BSA and Azide free (AB251103)

This data was developed using ab192020, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-ARHGAP25 antibody [EPR13233-60] - BSA and Azide free (ab251103) at 1/1000 dilution

Lane 1:

Ramos cell lysate at 20 µg

Lane 2:

Raji cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 73 kDa

Observed band size: 77 kDa

false

Unconjugated

Anti-ARHGAP25 antibody [EPR13233-60]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR13233-60

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Product details

ab251103 is the carrier-free version of ab192020.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ARHGAP25 also known as Rho GTPase-activating protein 25 functions mechanically as a GTPase activator for the Rho-family of small GTPases. This protein plays a role in switching these GTPases from an active state to an inactive state by stimulating the intrinsic GTPase activity. ARHGAP25 has a mass of approximately 61 kDa. It is expressed mainly in hematopoietic cells with specific expression in leukocytes. Its activity is important in cellular processes involving actin cytoskeletal reorganization.

Biological function summary

ARHGAP25 modulates the actin cytoskeleton impacting cell migration phagocytosis and other hematopoietic cell functions. It acts as a part of regulatory complexes that control cytoskeletal dynamics and cellular morphology. By regulating Rho family members like Rac1 and Cdc42 ARHGAP25 influences actin polymerization and cellular movement particularly in immune cells. This protein contributes to maintaining the integrity of immune responses.

Pathways

ARHGAP25 plays a role in the Rho GTPase signaling pathway and interacts with pathways that control cell division and migration. Within these networks ARHGAP25 interacts closely with proteins such as Rac1 and Cdc42 which are widely involved in actin dynamics and cell motility. This interaction impacts pathways that govern the reorganization of actin filaments important for leukocyte function and migration.

ARHGAP25 is involved in conditions like chronic inflammatory diseases and immune system dysregulation. Its dysregulated expression or function can lead to altered immune cell behavior and contribute to chronic inflammation. The protein's relationship with Rac1 and Cdc42 is significant as these proteins also associate with pathways implicated in immune dysfunction and inflammation. Understanding ARHGAP25's role provides insight into targeting therapies for disorders characterized by abnormal immune responses.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

GTPase activator for the Rho-type GTPases by converting them to an inactive GDP-bound state.

See full target information ARHGAP25

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-ARHGAP25 antibody [EPR13233-60] - BSA and Azide free

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARHGAP25 antibody [EPR13233-60] - BSA and Azide free (AB251103)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARHGAP25 antibody [EPR13233-60] - BSA and Azide free (AB251103)

Western blot - Anti-ARHGAP25 antibody [EPR13233-60] - BSA and Azide free (AB251103)

Western blot - Anti-ARHGAP25 antibody [EPR13233-60] - BSA and Azide free (AB251103)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Product promise