AB181202

Anti-ARHGAP25 antibody [EPR13233]

RabMAb
Recombinant
What is this?

Be the first to review this product! Submit a review

(3 Publications)

Rabbit Recombinant Monoclonal ARHGAP25 antibody. Suitable for IP, WB, ICC/IF and reacts with Human samples. Cited in 3 publications.

View Alternative Names

KIAA0053, ARHGAP25, Rho GTPase-activating protein 25, Rho-type GTPase-activating protein 25

4 Images

Immunocytochemistry/ Immunofluorescence - Anti-ARHGAP25 antibody [EPR13233] (AB181202)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-ARHGAP25 antibody [EPR13233] (AB181202)

Immunofluorescent analysis of Jurkat cells (-20°C Acetone-fixed) labeling ARHGAP25 with ab181202 at 1/100 dilution followed by Goat anti rabbit IgG (AlexaFluor® 488) secondary at 1/200 dilution and counter-stained with DAPI (blue).

Supplier Data

Immunoprecipitation - Anti-ARHGAP25 antibody [EPR13233] (AB181202)

Western blot analysis of immunoprecipitation pellet from Jurkat cell lysate immunoprecipitated using ab181202 at 1/50 dilution.
Secondary : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution.

All lanes:

Immunoprecipitation - Anti-ARHGAP25 antibody [EPR13233] (ab181202)

Predicted band size: 65 kDa,73 kDa

false

Supplier Data

Western blot - Anti-ARHGAP25 antibody [EPR13233] (AB181202)

All lanes:

Western blot - Anti-ARHGAP25 antibody [EPR13233] (ab181202) at 1/10000 dilution

All lanes:

Ramos cell lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 73 kDa

false

Supplier Data

Western blot - Anti-ARHGAP25 antibody [EPR13233] (AB181202)

All lanes:

Western blot - Anti-ARHGAP25 antibody [EPR13233] (ab181202) at 1/10000 dilution

Lane 1:

Daudi cell lysate at 20 µg

Lane 2:

Jurkat cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 73 kDa

false

Carrier free

Anti-ARHGAP25 antibody [EPR13233] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR13233

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, IP, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/30 - 1/50", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000 - 1/50000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50 - 1/100", "ICCIF-species-notes": "" } } }

style

left-column

Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ARHGAP25 also known as Rho GTPase-activating protein 25 functions mechanically as a GTPase activator for the Rho-family of small GTPases. This protein plays a role in switching these GTPases from an active state to an inactive state by stimulating the intrinsic GTPase activity. ARHGAP25 has a mass of approximately 61 kDa. It is expressed mainly in hematopoietic cells with specific expression in leukocytes. Its activity is important in cellular processes involving actin cytoskeletal reorganization.

Biological function summary

ARHGAP25 modulates the actin cytoskeleton impacting cell migration phagocytosis and other hematopoietic cell functions. It acts as a part of regulatory complexes that control cytoskeletal dynamics and cellular morphology. By regulating Rho family members like Rac1 and Cdc42 ARHGAP25 influences actin polymerization and cellular movement particularly in immune cells. This protein contributes to maintaining the integrity of immune responses.

Pathways

ARHGAP25 plays a role in the Rho GTPase signaling pathway and interacts with pathways that control cell division and migration. Within these networks ARHGAP25 interacts closely with proteins such as Rac1 and Cdc42 which are widely involved in actin dynamics and cell motility. This interaction impacts pathways that govern the reorganization of actin filaments important for leukocyte function and migration.

ARHGAP25 is involved in conditions like chronic inflammatory diseases and immune system dysregulation. Its dysregulated expression or function can lead to altered immune cell behavior and contribute to chronic inflammation. The protein's relationship with Rac1 and Cdc42 is significant as these proteins also associate with pathways implicated in immune dysfunction and inflammation. Understanding ARHGAP25's role provides insight into targeting therapies for disorders characterized by abnormal immune responses.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

GTPase activator for the Rho-type GTPases by converting them to an inactive GDP-bound state.

See full target information ARHGAP25

style

left-column

Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Journal of gastrointestinal oncology 16:191-208 PubMed40115921

2025

PRMT5 attenuates regorafenib-induced DNA damage in hepatocellular carcinoma cells through symmetric dimethylation of RPL14.

Applications

Unspecified application

Species

Unspecified reactive species

Wendi Bi,Xiaojuan Sun,Qiuyun Yi,Xinyu Jiang,Huisi He,Lixuan Jiang,Zhecai Fan,Hailing Huang,Wen Wen,Xiaoqing Jiang

Frontiers in immunology 14:1202725 PubMed37465666

2023

Prognostic and immunological significance of an M1 macrophage-related gene signature in osteosarcoma.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaoyu Mao,Fanglong Song,Ju Jin,Bin Zou,Peijun Dai,Mingjuan Sun,Weicheng Xu,Lianghua Wang,Yifan Kang

International journal of molecular medicine 47:659-667 PubMed33416153

2021

Downregulation of long non‑coding RNA GACAT1 suppresses proliferation and induces apoptosis of NSCLC cells by sponging microRNA‑422a.

Applications

Unspecified application

Species

Unspecified reactive species

Youqing Zhong,Hui Lin,Qi Li,Chang Liu,Lei Zhong

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-ARHGAP25 antibody [EPR13233]

Immunocytochemistry/ Immunofluorescence - Anti-ARHGAP25 antibody [EPR13233] (AB181202)

Immunoprecipitation - Anti-ARHGAP25 antibody [EPR13233] (AB181202)

Western blot - Anti-ARHGAP25 antibody [EPR13233] (AB181202)

Western blot - Anti-ARHGAP25 antibody [EPR13233] (AB181202)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

Alternative Products

Primary Antibodies

Complementary Products

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Product promise