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AB250423

Anti-ARHGAP25 antibody [EPR13233] - BSA and Azide free

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(1 Publication)

Rabbit Recombinant Monoclonal ARHGAP25 antibody. Carrier free. Suitable for ICC/IF, IP, WB and reacts with Human samples. Cited in 1 publication.

View Alternative Names

KIAA0053, ARHGAP25, Rho GTPase-activating protein 25, Rho-type GTPase-activating protein 25

4 Images
Immunocytochemistry/ Immunofluorescence - Anti-ARHGAP25 antibody [EPR13233] - BSA and Azide free (AB250423)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-ARHGAP25 antibody [EPR13233] - BSA and Azide free (AB250423)

This data was developed using ab181202, the same antibody clone in a different buffer formulation.

Immunofluorescent analysis of Jurkat cells (-20°C Acetone-fixed) labeling ARHGAP25 with ab181202 at 1/100 dilution followed by Goat anti rabbit IgG (AlexaFluor® 488) secondary at 1/200 dilution and counter-stained with DAPI (blue).

Immunoprecipitation - Anti-ARHGAP25 antibody [EPR13233] - BSA and Azide free (AB250423)
  • IP

Supplier Data

Immunoprecipitation - Anti-ARHGAP25 antibody [EPR13233] - BSA and Azide free (AB250423)

This data was developed using ab181202, the same antibody clone in a different buffer formulation.

Western blot analysis of immunoprecipitation pellet from Jurkat cell lysate immunoprecipitated using ab181202 at 1/50 dilution.
Secondary : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution.

All lanes:

Immunoprecipitation - Anti-ARHGAP25 antibody [EPR13233] (<a href='/en-us/products/primary-antibodies/arhgap25-antibody-epr13233-ab181202'>ab181202</a>)

Predicted band size: 65 kDa,73 kDa

false

Western blot - Anti-ARHGAP25 antibody [EPR13233] - BSA and Azide free (AB250423)
  • WB

Supplier Data

Western blot - Anti-ARHGAP25 antibody [EPR13233] - BSA and Azide free (AB250423)

This data was developed using ab181202, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-ARHGAP25 antibody [EPR13233] (<a href='/en-us/products/primary-antibodies/arhgap25-antibody-epr13233-ab181202'>ab181202</a>) at 1/10000 dilution

All lanes:

Ramos cell lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 73 kDa

false

Western blot - Anti-ARHGAP25 antibody [EPR13233] - BSA and Azide free (AB250423)
  • WB

Supplier Data

Western blot - Anti-ARHGAP25 antibody [EPR13233] - BSA and Azide free (AB250423)

This data was developed using ab181202, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-ARHGAP25 antibody [EPR13233] (<a href='/en-us/products/primary-antibodies/arhgap25-antibody-epr13233-ab181202'>ab181202</a>) at 1/10000 dilution

Lane 1:

Daudi cell lysate at 20 µg

Lane 2:

Jurkat cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 73 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR13233

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB, ICC/IF, IP

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" } } }
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Product details

ab250423 is the carrier-free version of ab181202.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ARHGAP25 also known as Rho GTPase-activating protein 25 functions mechanically as a GTPase activator for the Rho-family of small GTPases. This protein plays a role in switching these GTPases from an active state to an inactive state by stimulating the intrinsic GTPase activity. ARHGAP25 has a mass of approximately 61 kDa. It is expressed mainly in hematopoietic cells with specific expression in leukocytes. Its activity is important in cellular processes involving actin cytoskeletal reorganization.
Biological function summary

ARHGAP25 modulates the actin cytoskeleton impacting cell migration phagocytosis and other hematopoietic cell functions. It acts as a part of regulatory complexes that control cytoskeletal dynamics and cellular morphology. By regulating Rho family members like Rac1 and Cdc42 ARHGAP25 influences actin polymerization and cellular movement particularly in immune cells. This protein contributes to maintaining the integrity of immune responses.

Pathways

ARHGAP25 plays a role in the Rho GTPase signaling pathway and interacts with pathways that control cell division and migration. Within these networks ARHGAP25 interacts closely with proteins such as Rac1 and Cdc42 which are widely involved in actin dynamics and cell motility. This interaction impacts pathways that govern the reorganization of actin filaments important for leukocyte function and migration.

ARHGAP25 is involved in conditions like chronic inflammatory diseases and immune system dysregulation. Its dysregulated expression or function can lead to altered immune cell behavior and contribute to chronic inflammation. The protein's relationship with Rac1 and Cdc42 is significant as these proteins also associate with pathways implicated in immune dysfunction and inflammation. Understanding ARHGAP25's role provides insight into targeting therapies for disorders characterized by abnormal immune responses.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

GTPase activator for the Rho-type GTPases by converting them to an inactive GDP-bound state.
See full target information ARHGAP25
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Evidence-based complementary and alternative medicine : eCAM 2022:7757833 PubMed36091585

2022

Esculentoside A Alleviates Intestinal Dysmotility in Ulcerative Colitis by Regulating HS/CSE and NO/nNOS Systems.

Applications

Unspecified application

Species

Unspecified reactive species

Ying Liu,Wenhua Wei,Shiwei Liang,Haicheng Fang,Jie Cao
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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