Rabbit Recombinant Monoclonal ARHGAP27 antibody. Carrier free. Suitable for IHC-P, ICC/IF, WB, Flow Cyt (Intra) and reacts with Human samples.
Images
![Western blot - Anti-ARHGAP27 antibody [EPR12250] - BSA and Azide free (AB249685), expandable thumbnail](https://content.abcam.com/products/images/arhgap27-antibody-epr12250-bsa-and-azide-free-ab249685--western-blot-img71098.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-ARHGAP27 antibody [EPR12250] - BSA and Azide free (AB249685)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARHGAP27 antibody [EPR12250] - BSA and Azide free (AB249685), expandable thumbnail](https://content.abcam.com/products/images/arhgap27-antibody-epr12250-bsa-and-azide-free-ab249685--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img31030.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARHGAP27 antibody [EPR12250] - BSA and Azide free (AB249685)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARHGAP27 antibody [EPR12250] - BSA and Azide free (AB249685), expandable thumbnail](https://content.abcam.com/products/images/arhgap27-antibody-epr12250-bsa-and-azide-free-ab249685--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img107964.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARHGAP27 antibody [EPR12250] - BSA and Azide free (AB249685)")
![Immunocytochemistry/ Immunofluorescence - Anti-ARHGAP27 antibody [EPR12250] - BSA and Azide free (AB249685), expandable thumbnail](https://content.abcam.com/products/images/arhgap27-antibody-epr12250-bsa-and-azide-free-ab249685--immunocytochemistry-immunofluorescence-img968.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-ARHGAP27 antibody [EPR12250] - BSA and Azide free (AB249685)")
![Flow Cytometry (Intracellular) - Anti-ARHGAP27 antibody [EPR12250] - BSA and Azide free (AB249685), expandable thumbnail](https://content.abcam.com/products/images/arhgap27-antibody-epr12250-bsa-and-azide-free-ab249685--flow-cytometry-intracellular-img26318.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-ARHGAP27 antibody [EPR12250] - BSA and Azide free (AB249685)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IHC-P | ICC/IF | IP | WB | Flow Cyt (Intra) | |
|---|---|---|---|---|---|
| Human | Tested | Tested | Not recommended | Tested | Tested |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Target data
Function
Rho GTPase-activating protein which may be involved in clathrin-mediated endocytosis. GTPase activators for the Rho-type GTPases act by converting them to an inactive GDP-bound state. Has activity toward CDC42 and RAC1 (By similarity).
Alternative names
CAMGAP1, SH3D20, PP905, ARHGAP27, Rho GTPase-activating protein 27, CIN85-associated multi-domain-containing Rho GTPase-activating protein 1, Rho-type GTPase-activating protein 27, SH3 domain-containing protein 20
Recommended products
Rabbit Recombinant Monoclonal ARHGAP27 antibody. Carrier free. Suitable for IHC-P, ICC/IF, WB, Flow Cyt (Intra) and reacts with Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR12250
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab249685 is the carrier-free version of Anti-ARHGAP27 antibody [EPR12250] ab171973.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
ARHGAP27 also known as Rho GTPase-activating protein 27 is a regulatory protein with a mass of approximately 88 kDa. It modulates the activity of Rho family GTPases turning them from an active GTP-bound state to an inactive GDP-bound state. This protein mainly expresses in the brain and testis suggesting its specialized roles in these tissues. By controlling the actin cytoskeleton ARHGAP27 influences cell shape motility and division.
- Biological function summary
The regulation of cytoskeletal dynamics involves ARHGAP27 in cellular processes. It plays a part in endocytosis a process important for internalizing molecules from the cell surface. ARHGAP27 functions in coordination with other GAPs (GTPase-activating proteins) to achieve its role. It does not operate singularly but can interact within a larger protein complex to exert its function effectively.
- Pathways
ARHGAP27 participates within the regulation of Rho GTPase cycle pathway. This pathway is significant for actin cytoskeleton organization. ARHGAP27 interfaces with other proteins like Cdc42 and Rac1 which play pivotal roles in signal transduction related to cytoskeletal arrangements and cellular responses. The protein's modulation of these GTPases impacts intracellular signaling cascades that adjust the organization of actin filaments.
- Associated diseases and disorders
ARHGAP27 associations relate to neurodevelopmental conditions and certain cancers. Mutations or dysregulation of ARHGAP27 may contribute to improper neural connections or tumor development. In some cancer studies researchers identify links between ARHGAP27 and the oncogenic protein RhoA. The interactions and pathways involving ARHGAP27 provide insights into its contributions to pathological states highlighting its potential as a biomarker or therapeutic target.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
5 product images
![Western blot - Anti-ARHGAP27 antibody [EPR12250] - BSA and Azide free (ab249685), expandable thumbnail](https://content.abcam.com/products/images/arhgap27-antibody-epr12250-bsa-and-azide-free-ab249685--western-blot-img71098.jpg "Western blot - Anti-ARHGAP27 antibody [EPR12250] - BSA and Azide free (ab249685)")
Western blot - Anti-ARHGAP27 antibody [EPR12250] - BSA and Azide free (ab249685)
This data was developed using Anti-ARHGAP27 antibody [EPR12250] ab171973, the same antibody clone in a different buffer formulation.
Note: primary antibody diluted in BSA.
All lanes: Western blot - Anti-ARHGAP27 antibody [EPR12250] (Anti-ARHGAP27 antibody [EPR12250] ab171973) at 1/1000 dilution
Lane 1: HepG2 cell lysate at 10 µg
Lane 2: A549 cell lysate at 10 µg
Lane 3: BxPC-3 cell lysate at 10 µg
Predicted band size: 98 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARHGAP27 antibody [EPR12250] - BSA and Azide free (ab249685), expandable thumbnail](https://content.abcam.com/products/images/arhgap27-antibody-epr12250-bsa-and-azide-free-ab249685--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img31030.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARHGAP27 antibody [EPR12250] - BSA and Azide free (ab249685)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARHGAP27 antibody [EPR12250] - BSA and Azide free (ab249685)
This data was developed using Anti-ARHGAP27 antibody [EPR12250] ab171973, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue labeling ARHGAP27 with Anti-ARHGAP27 antibody [EPR12250] ab171973 at 1/100 dilution. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARHGAP27 antibody [EPR12250] - BSA and Azide free (ab249685), expandable thumbnail](https://content.abcam.com/products/images/arhgap27-antibody-epr12250-bsa-and-azide-free-ab249685--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img107964.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARHGAP27 antibody [EPR12250] - BSA and Azide free (ab249685)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARHGAP27 antibody [EPR12250] - BSA and Azide free (ab249685)
This data was developed using Anti-ARHGAP27 antibody [EPR12250] ab171973, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human pancreas tissue labeling ARHGAP27 with Anti-ARHGAP27 antibody [EPR12250] ab171973 at 1/100 dilution. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
![Immunocytochemistry/ Immunofluorescence - Anti-ARHGAP27 antibody [EPR12250] - BSA and Azide free (ab249685), expandable thumbnail](https://content.abcam.com/products/images/arhgap27-antibody-epr12250-bsa-and-azide-free-ab249685--immunocytochemistry-immunofluorescence-img968.jpg "Immunocytochemistry/ Immunofluorescence - Anti-ARHGAP27 antibody [EPR12250] - BSA and Azide free (ab249685)")
Immunocytochemistry/ Immunofluorescence - Anti-ARHGAP27 antibody [EPR12250] - BSA and Azide free (ab249685)
This data was developed using Anti-ARHGAP27 antibody [EPR12250] ab171973, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of BxPC-3 cells labeling ARHGAP27 with Anti-ARHGAP27 antibody [EPR12250] ab171973 (green) at 1/50 dilution. DAPI nuclear staining (blue).
![Flow Cytometry (Intracellular) - Anti-ARHGAP27 antibody [EPR12250] - BSA and Azide free (ab249685), expandable thumbnail](https://content.abcam.com/products/images/arhgap27-antibody-epr12250-bsa-and-azide-free-ab249685--flow-cytometry-intracellular-img26318.jpg "Flow Cytometry (Intracellular) - Anti-ARHGAP27 antibody [EPR12250] - BSA and Azide free (ab249685)")
Flow Cytometry (Intracellular) - Anti-ARHGAP27 antibody [EPR12250] - BSA and Azide free (ab249685)
This data was developed using Anti-ARHGAP27 antibody [EPR12250] ab171973, the same antibody clone in a different buffer formulation.
Intracellular flow cytometric analysis of permeabilized A549 cells labeling ARHGAP27 with Anti-ARHGAP27 antibody [EPR12250] ab171973 at 1/10 dilution (red) or a Rabbit IgG (negative) (green).
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