JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB85853

Anti-ARHGAP29 antibody

Be the first to review this product! Submit a review

|

(3 Publications)

Rabbit Polyclonal ARHGAP29 antibody. Suitable for WB, IP and reacts with Mouse, Human samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human ARHGAP29 aa 1200 to C-terminus.

View Alternative Names

PARG1, ARHGAP29, Rho GTPase-activating protein 29, PTPL1-associated RhoGAP protein 1, Rho-type GTPase-activating protein 29

2 Images
Immunoprecipitation - Anti-ARHGAP29 antibody (AB85853)
  • IP

Unknown

Immunoprecipitation - Anti-ARHGAP29 antibody (AB85853)

Detection of ARHGAP29 by Western Blot of Immunprecipitate.
ab85853, at 1 μg/ml, staining ARHGAP29 in HeLa whole cell lysate immunoprecipitated using ab85853 at 3μg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Detection : Chemiluminescence with exposure time of 10 seconds.

All lanes:

Immunoprecipitation - Anti-ARHGAP29 antibody (ab85853)

Predicted band size: 142 kDa

false

Western blot - Anti-ARHGAP29 antibody (AB85853)
  • WB

Unknown

Western blot - Anti-ARHGAP29 antibody (AB85853)

All lanes:

Western blot - Anti-ARHGAP29 antibody (ab85853) at 0.4 µg/mL

Lane 1:

HeLa whole cell lysate at 50 µg

Lane 2:

HeLa whole cell lysate at 15 µg

Lane 3:

HeLa whole cell lysate at 5 µg

Lane 4:

293T whole cell lysate at 50 µg

Lane 5:

NIH3T3 whole cell lysate at 50 µg

Predicted band size: 142 kDa

Observed band size: 170 kDa

true

Exposure time: 30s

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

IP, WB

applications

Immunogen

Synthetic Peptide within Human ARHGAP29 aa 1200 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q52LW3

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "<p></p>", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-5 µg/mg of lysate", "IP-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "<p></p>", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "" } } }
style
left-column
style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
ab85853 was affinity purified using an epitope specific to ARHGAP29 immobilized on solid support.
Storage buffer
pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: Tris citrate/phosphate
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ARHGAP29 also known as Rho GTPase Activating Protein 29 is a protein involved in regulating the actin cytoskeleton through its action on Rho family GTPases. This protein has a molecular mass of approximately 145 kDa. It is expressed in various tissues including the heart and the brain. ARHGAP29 acts by stimulating GTPase activity resulting in the inactivation of Rho GTPases which are important for cell morphology migration and adhesion processes.
Biological function summary

ARHGAP29 plays roles in modulating cellular structures and motility by regulating Rho GTPase activity. It functions as a GTPase-activating protein that converts active GTP-bound RhoA Rac1 and Cdc42 to their inactive GDP-bound states. ARHGAP29 is not part of any known major protein complex but interacts with signal transduction molecules to exert its effects on the cytoskeleton.

Pathways

ARHGAP29 is an important regulator within the Rho GTPase signaling pathway influencing actin dynamics that affect cell movement and shape. This protein interacts with and modulates RhoA activity which is involved in the MAPK signaling pathway linking it to broader cellular functions such as growth and differentiation. ARHGAP29 has a relationship with other GTPase-activating proteins collectively contributing to the regulation of the Rho signaling pathway.

ARHGAP29 has been associated with particular conditions like nonsyndromic cleft lip with or without cleft palate and vascular diseases. Mutations or dysregulation of ARHGAP29 can impact cytoskeletal organization influencing tissue morphogenesis and vascular integrity. It connects to other proteins like RhoA whose abnormal regulation often results in similar developmental and vascular disorders.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

GTPase activator for the Rho-type GTPases by converting them to an inactive GDP-bound state. Has strong activity toward RHOA, and weaker activity toward RAC1 and CDC42. May act as a specific effector of RAP2A to regulate Rho. In concert with RASIP1, suppresses RhoA signaling and dampens ROCK and MYH9 activities in endothelial cells and plays an essential role in blood vessel tubulogenesis.
See full target information ARHGAP29
style
left-column

Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Oncology reports 44:2735-2745 PubMed33125156

2020

ARHGAP29 expression may be a novel prognostic factor of cell proliferation and invasion in prostate cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Kosuke Shimizu,Hiroaki Matsumoto,Hiroshi Hirata,Koji Ueno,Masahiro Samoto,Junichi Mori,Nakanori Fujii,Yoshihisa Kawai,Ryo Inoue,Yoshiaki Yamamoto,Seiji Yano,Tomoyuki Shimabukuro,Makoto Furutani-Seiki,Hideyasu Matsuyama

Journal of molecular neuroscience : MN 69:570-579 PubMed31486971

2019

PINK1 Silencing Modifies Dendritic Spine Dynamics of Mouse Hippocampal Neurons.

Applications

Unspecified application

Species

Unspecified reactive species

C J Hernández,C Báez-Becerra,M J Contreras-Zárate,H Arboleda,G Arboleda

Journal of biological rhythms 33:350-362 PubMed29845885

2018

Regulation of Pol II Pausing Is Involved in Daily Gene Transcription in the Mouse Liver.

Applications

Unspecified application

Species

Unspecified reactive species

Jialou Zhu,Chengwei Li,Changxia Gong,Xiaodong Li
View all publications
style
left-column
style
left-column
style
right-column

Complementary Products

Primary Antibodies

AB187027

Anti-RhoA antibody [EPR18134]

RabMAb|Recombinant|KO Validated

Flow Cytometry (Intracellular) - Anti-RhoA antibody [EPR18134] (AB187027)

  • 4
  • 1
Primary Antibodies

AB78910

Anti-Zyxin (phospho S142) antibody

Western blot - Anti-Zyxin (phospho S142) antibody (AB78910)

  • 0
  • 0
Primary Antibodies

AB9485

Anti-GAPDH antibody - Loading Control

BOND RX™ Validated|Lab Essentials

Immunocytochemistry/ Immunofluorescence - Anti-GAPDH antibody - Loading Control (AB9485)

  • 5
  • 88
Primary Antibodies

AB273052

Anti-ZWINT antibody [EPR23440-96] - BSA and Azide free

BOND RX™ Validated|RabMAb|Recombinant

Flow Cytometry (Intracellular) - Anti-ZWINT antibody [EPR23440-96] - BSA and Azide free (AB273052)

  • 0
  • 0

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com