Rabbit Polyclonal ARHGAP4 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human ARHGAP4 aa 1-250.
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine
WB | ICC/IF | |
---|---|---|
Human | Tested | Tested |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1/500.00000 - 1/3000.00000 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1/100.00000 - 1/200.00000 | Notes - |
Inhibitory effect on stress fiber organization. May down-regulate Rho-like GTPase in hematopoietic cells.
KIAA0131, RGC1, RHOGAP4, ARHGAP4, Rho GTPase-activating protein 4, Rho-GAP hematopoietic protein C1, Rho-type GTPase-activating protein 4, p115
Rabbit Polyclonal ARHGAP4 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human ARHGAP4 aa 1-250.
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine
ARHGAP4 also known as Rho GTPase-activating protein 4 is an important regulator in cellular signaling involved in modulating the activity of Rho family GTPases. These proteins play a role in remodelization of the actin cytoskeleton cellular migration and morphogenes. ARHGAP4 has a molecular mass of approximately 113 kDa. It expresses in various tissues with notable expression in the brain spleen and lymphoid organs suggesting its functions in different biological processes and systems.
ARHGAP4 contributes significantly to the regulation of actin dynamics and cell morphology. This protein is not typically part of a complex but interacts with other proteins to control cell structure and movement. Its activity influences importance in neuronal development and immune response indicating its diverse roles in cellular functions. Moreover it helps in controlling cell motility and adhesion affecting how cells interact with their environments and neighboring cells.
ARHGAP4 is intricately involved in the Rho GTPase cycle and the regulation of the actin cytoskeleton pathway. This protein acts as a nexus by inactivating Rho GTPases such as RhoA Rac1 and Cdc42 modulating the signals that lead to cytoskeletal rearrangement. Such interactions find relevance in pathways that orchestrate cellular movement and stabilization significantly impacting pathways that control cell shape and motility.
ARHGAP4 shows associations with neurological disorders and certain cancers. Alterations in its expression or activity are linked to developmental brain abnormalities affecting neuronal migration and connectivity. In cancer its dysregulation may lead to changes in tumor cell motility promoting invasion and metastasis. Proteins such as RhoA and Rac1 which interact with ARHGAP4 are also involved in these diseases highlighting the importance of Rho GTPase signaling pathways in disease pathology.
We have tested this species and application combination and it works. It is covered by our product promise.
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
7.5% SDS PAGE
All lanes: Western blot - Anti-ARHGAP4 antibody (ab96251) at 1/1000 dilution
All lanes: Jurkat whole cell lysate at 30 µg
Predicted band size: 105 kDa
Immunofluorescence analysis of paraformaldehyde-fixed A549 cells, using sb96251 at 1/200 dilution.
Lower image is merged with DNA probe.
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com