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AB96251

Anti-ARHGAP4 antibody

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(1 Publication)

Rabbit Polyclonal ARHGAP4 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human ARHGAP4 aa 1-250.

View Alternative Names

KIAA0131, RGC1, RHOGAP4, ARHGAP4, Rho GTPase-activating protein 4, Rho-GAP hematopoietic protein C1, Rho-type GTPase-activating protein 4, p115

2 Images
Immunocytochemistry/ Immunofluorescence - Anti-ARHGAP4 antibody (AB96251)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-ARHGAP4 antibody (AB96251)

Immunofluorescence analysis of paraformaldehyde-fixed A549 cells, using sb96251 at 1/200 dilution. Lower image is merged with DNA probe.

Western blot - Anti-ARHGAP4 antibody (AB96251)
  • WB

Unknown

Western blot - Anti-ARHGAP4 antibody (AB96251)

7.5% SDS PAGE

All lanes:

Western blot - Anti-ARHGAP4 antibody (ab96251) at 1/1000 dilution

All lanes:

Jurkat whole cell lysate at 30 µg

Predicted band size: 105 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human ARHGAP4 aa 1-250. The exact immunogen used to generate this antibody is proprietary information.

P98171

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ARHGAP4 also known as Rho GTPase-activating protein 4 is an important regulator in cellular signaling involved in modulating the activity of Rho family GTPases. These proteins play a role in remodelization of the actin cytoskeleton cellular migration and morphogenes. ARHGAP4 has a molecular mass of approximately 113 kDa. It expresses in various tissues with notable expression in the brain spleen and lymphoid organs suggesting its functions in different biological processes and systems.
Biological function summary

ARHGAP4 contributes significantly to the regulation of actin dynamics and cell morphology. This protein is not typically part of a complex but interacts with other proteins to control cell structure and movement. Its activity influences importance in neuronal development and immune response indicating its diverse roles in cellular functions. Moreover it helps in controlling cell motility and adhesion affecting how cells interact with their environments and neighboring cells.

Pathways

ARHGAP4 is intricately involved in the Rho GTPase cycle and the regulation of the actin cytoskeleton pathway. This protein acts as a nexus by inactivating Rho GTPases such as RhoA Rac1 and Cdc42 modulating the signals that lead to cytoskeletal rearrangement. Such interactions find relevance in pathways that orchestrate cellular movement and stabilization significantly impacting pathways that control cell shape and motility.

ARHGAP4 shows associations with neurological disorders and certain cancers. Alterations in its expression or activity are linked to developmental brain abnormalities affecting neuronal migration and connectivity. In cancer its dysregulation may lead to changes in tumor cell motility promoting invasion and metastasis. Proteins such as RhoA and Rac1 which interact with ARHGAP4 are also involved in these diseases highlighting the importance of Rho GTPase signaling pathways in disease pathology.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Inhibitory effect on stress fiber organization. May down-regulate Rho-like GTPase in hematopoietic cells.
See full target information ARHGAP4
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Science advances 9:eadf2746 PubMed37390205

2023

A localized hydrogel-mediated chemotherapy causes immunogenic cell death via activation of ceramide-mediated unfolded protein response.

Applications

Unspecified application

Species

Unspecified reactive species

Animesh Kar,Dolly Jain,Sandeep Kumar,Kajal Rajput,Sanjay Pal,Kajal Rana,Raunak Kar,Somesh K Jha,Nihal Medatwal,Prabhu Srinivas Yavvari,Nishant Pandey,Devashish Mehta,Harsh Sharma,Debanjan Bhattacharya,Manas K Pradhan,Ravi Datta Sharma,Aasheesh Srivastava,Usha Agrawal,Arnab Mukhopadhyay,Sagar Sengupta,Veena S Patil,Avinash Bajaj,Ujjaini Dasgupta
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

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