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Rabbit Polyclonal ARHGAP4 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human ARHGAP4 aa 1-250.

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Images

Western blot - Anti-ARHGAP4 antibody (AB96251), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-ARHGAP4 antibody (AB96251), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine

Form
Liquid
Clonality
Polyclonal

Immunogen

  • Recombinant Fragment Protein within Human ARHGAP4 aa 1-250. The exact immunogen used to generate this antibody is proprietary information. Database link P98171

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBICC/IF
Human
Tested
Tested

Tested
Tested

Species
Human
Dilution info
1/500.00000 - 1/3000.00000
Notes

-

Tested
Tested

Species
Human
Dilution info
1/100.00000 - 1/200.00000
Notes

-

Target data

Function

Inhibitory effect on stress fiber organization. May down-regulate Rho-like GTPase in hematopoietic cells.

Alternative names

Recommended products

Rabbit Polyclonal ARHGAP4 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human ARHGAP4 aa 1-250.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Polyclonal
Immunogen
  • Recombinant Fragment Protein within Human ARHGAP4 aa 1-250. The exact immunogen used to generate this antibody is proprietary information. Database link P98171
Purification technique
Affinity purification Immunogen
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

ARHGAP4 also known as Rho GTPase-activating protein 4 is an important regulator in cellular signaling involved in modulating the activity of Rho family GTPases. These proteins play a role in remodelization of the actin cytoskeleton cellular migration and morphogenes. ARHGAP4 has a molecular mass of approximately 113 kDa. It expresses in various tissues with notable expression in the brain spleen and lymphoid organs suggesting its functions in different biological processes and systems.

Biological function summary

ARHGAP4 contributes significantly to the regulation of actin dynamics and cell morphology. This protein is not typically part of a complex but interacts with other proteins to control cell structure and movement. Its activity influences importance in neuronal development and immune response indicating its diverse roles in cellular functions. Moreover it helps in controlling cell motility and adhesion affecting how cells interact with their environments and neighboring cells.

Pathways

ARHGAP4 is intricately involved in the Rho GTPase cycle and the regulation of the actin cytoskeleton pathway. This protein acts as a nexus by inactivating Rho GTPases such as RhoA Rac1 and Cdc42 modulating the signals that lead to cytoskeletal rearrangement. Such interactions find relevance in pathways that orchestrate cellular movement and stabilization significantly impacting pathways that control cell shape and motility.

Associated diseases and disorders

ARHGAP4 shows associations with neurological disorders and certain cancers. Alterations in its expression or activity are linked to developmental brain abnormalities affecting neuronal migration and connectivity. In cancer its dysregulation may lead to changes in tumor cell motility promoting invasion and metastasis. Proteins such as RhoA and Rac1 which interact with ARHGAP4 are also involved in these diseases highlighting the importance of Rho GTPase signaling pathways in disease pathology.

Product promise

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2 product images

  • Western blot - Anti-ARHGAP4 antibody (ab96251), expandable thumbnail

    Western blot - Anti-ARHGAP4 antibody (ab96251)

    7.5% SDS PAGE

    All lanes: Western blot - Anti-ARHGAP4 antibody (ab96251) at 1/1000 dilution

    All lanes: Jurkat whole cell lysate at 30 µg

    Predicted band size: 105 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-ARHGAP4 antibody (ab96251), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-ARHGAP4 antibody (ab96251)

    Immunofluorescence analysis of paraformaldehyde-fixed A549 cells, using sb96251 at 1/200 dilution.
    Lower image is merged with DNA probe.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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