Rabbit Polyclonal ARHGAP42 antibody. Suitable for WB, IHC-P and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human ARHGAP42 aa 500-750.
Images
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.03% Proclin 300
Constituents: PBS, 50% Glycerol (glycerin, glycerine)- Form
Liquid
- Clonality
Polyclonal
Immunogen
- Recombinant Fragment Protein within Human ARHGAP42 aa 500-750. The exact immunogen used to generate this antibody is proprietary information. Database link A6NI28
Reactivity data
Select an application| WB | IHC-P | |
|---|---|---|
| Human | Tested | Tested |
| Mouse | Predicted | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/2000 - 1/10000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/20 - 1/200 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
Associated Products
Select an associated product type
1 products for Alternative Product
Target data
Function
May influence blood pressure by functioning as a GTPase-activating protein for RHOA in vascular smooth muscle.
Alternative names
Rho GTPase-activating protein 42, Rho GTPase-activating protein 10-like, Rho-type GTPase-activating protein 42, TMEM133, ARHGAP42, GRAF3
Recommended products
Rabbit Polyclonal ARHGAP42 antibody. Suitable for WB, IHC-P and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human ARHGAP42 aa 500-750.
Alternative names
Rho GTPase-activating protein 42, Rho GTPase-activating protein 10-like, Rho-type GTPase-activating protein 42, TMEM133, ARHGAP42, GRAF3
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.03% Proclin 300
Constituents: PBS, 50% Glycerol (glycerin, glycerine)- Form
Liquid
- Clonality
Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human ARHGAP42 aa 500-750. The exact immunogen used to generate this antibody is proprietary information. Database link A6NI28
- Purification technique
Affinity purification Protein G
- Concentration
- Purification notes
Purity >95%.
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage duration
1-2 weeks
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
ARHGAP42 also known as Rho GTPase-activating protein 42 is involved in regulating the Rho family of GTPases. This protein functions by stimulating the GTPase activity leading to the conversion of active GTP-bound Rho to its inactive GDP-bound form. The molecular mass of ARHGAP42 is approximately 106 kDa. Expression of ARHGAP42 occurs in various tissues notably in vascular smooth muscle cells. It plays a significant role in cellular processes through its regulatory activity.
- Biological function summary
ARHGAP42 influences the dynamics of actin cytoskeleton and cell shape by acting within the Rho GTPase family network. It may form part of larger protein complexes where it interacts with other cellular proteins to modulate cellular functions critical for maintaining cellular integrity and motility. Through its activity ARHGAP42 contributes to the regulation of vascular tone and blood pressure which are important in cardiovascular physiology.
- Pathways
ARHGAP42 serves as an integral component in the Rho signaling pathway. This pathway is essential for the regulation of cytoskeletal remodeling and is important for processes such as cell migration and morphology. ARHGAP42 interacts with associated proteins like RhoA a prominent member of the Rho GTPase family to exert its function within the Rho signaling cascade. Additionally it intersects with the vascular smooth muscle contraction pathway further supporting its role in cardiovascular system regulation.
- Associated diseases and disorders
ARHGAP42 links closely to hypertension and cardiovascular disease. Its regulation of vascular tone implicates it as a basis for understanding hypertensive disorders. Imbalance in ARHGAP42 function can lead to abnormal blood pressure regulation. Also interactions between ARHGAP42 and RhoA advance the understanding of such disorders since disruptions in the Rho pathway can affect normal cardiovascular functions.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
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3 product images
Western blot - Anti-ARHGAP42 antibody (ab222815)
All lanes: Western blot - Anti-ARHGAP42 antibody (ab222815) at 1/2000 dilution
All lanes: Human placenta lysate
SecondaryAll lanes: Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 99 kDa
Observed band size: 99 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARHGAP42 antibody (ab222815)
Paraffin-embedded human brain tissue stained for ARHGAP42 using ab222815 at 1/100 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARHGAP42 antibody (ab222815)
Paraffin-embedded human testis tissue stained for ARHGAP42 using ab222815 at 1/100 dilution in immunohistochemical analysis.
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