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AB101361

Anti-ARHGAP9 antibody

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(1 Publication)

Rabbit Polyclonal ARHGAP9 antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human Rho GTPase-activating protein 9 aa 50-300.

View Alternative Names

Rho GTPase-activating protein 9, Rho-type GTPase-activating protein 9, ARHGAP9

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARHGAP9 antibody (AB101361)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARHGAP9 antibody (AB101361)

Immunohistochemical analysis of paraffin-embedded skhep1 xenograft tissue with ab101361 at a 1/500 dilution.

Antigen retrieval : Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Western blot - Anti-ARHGAP9 antibody (AB101361)
  • WB

Unknown

Western blot - Anti-ARHGAP9 antibody (AB101361)

7.5% SDS-PAGE

All lanes:

Western blot - Anti-ARHGAP9 antibody (ab101361) at 1/2000 dilution

All lanes:

Raji cell lysate at 30 µg

Predicted band size: 83 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human Rho GTPase-activating protein 9 aa 50-300. The exact immunogen used to generate this antibody is proprietary information.

Q9BRR9

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 20% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ARHGAP9 also known as Rho GTPase activating protein 9 functions mainly as a GTPase-activating protein. It acts to regulate the Rho family of GTPases by accelerating their intrinsic GTPase activity leading to the inactivation of Rho proteins. ARHGAP9 is approximately 92 kDa in mass. Its expression is noted predominantly in hematopoietic tissues like the spleen and the lymph nodes suggesting its involvement in immune cell regulation.
Biological function summary

ARHGAP9 modulates cytoskeletal dynamics and cell movement influencing various cellular behaviors such as cell polarity migration and adhesion. The protein participates in the regulation of cell morphology and motility by affecting actin cytoskeleton organization. Although not a part of a specific complex ARHGAP9 interacts closely with other cytoskeletal regulators to execute these functions.

Pathways

ARHGAP9 engages significantly in the Rho GTPase signaling pathway influencing chemotaxis and actin filament reorganization. This interaction ties it with other related proteins like Rac1 and Cdc42 which play roles in modulating cell structure and movement. Additionally ARHGAP9 contributes to the modulation of the MAPK (Mitogen-Activated Protein Kinase) signaling pathway which impacts cell proliferation and differentiation processes.

Those affected by abnormal ARHGAP9 activity often include cancer and immune-related conditions. Abnormal regulation of ARHGAP9 can disrupt normal cell movement and proliferation leading to cancer progression and metastasis. In immune disorders its dysregulation may influence inflammatory responses and leukocyte function. Connections with other proteins such as RhoA and Rac1 are important in understanding its role in these disease contexts as they together affect pathways involved in cell survival and immune cell activity.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

GTPase activator for the Rho-type GTPases by converting them to an inactive GDP-bound state. Has a substantial GAP activity toward CDC42 and RAC1 and less toward RHOA. Has a role in regulating adhesion of hematopoietic cells to the extracellular matrix. Binds phosphoinositides, and has the highest affinity for phosphatidylinositol 3,4,5-trisphosphate, followed by phosphatidylinositol 3,4-bisphosphate and phosphatidylinositol 4,5-bisphosphate.
See full target information Rho GTPase-activating protein 9
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Oncology letters 17:965-973 PubMed30655855

2019

Ginsenoside Rg3 inhibits the migration and invasion of liver cancer cells by increasing the protein expression of ARHGAP9.

Applications

Unspecified application

Species

Unspecified reactive species

Meng-Yao Sun,Ya-Nan Song,Miao Zhang,Chun-Yan Zhang,Li-Jun Zhang,Hong Zhang
View all publications
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Product promise

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